1887

Abstract

SUMMARY

When Detroit cells are infected with poliovirus in the presence of 5 µg./ml. of actinomycin, cell-controlled RNA synthesis is blocked and 2-[C]uridine is incorporated only into poliovirus-specific RNA.

The addition of polyinosinic-polycytidylic acid (5 µg./ml.) under such conditions results in the inhibition of synthesis of 35 intracellular, infectious poliovirus RNA.

A high molecular weight fraction of poliovirus-specific RNA migrating on polyacrylamide gels, as would be expected of the double-stranded, replicative form of poliovirus RNA, is synthesized to the same extent as in the absence of interferon inducer. This RNA was digested with RNase and DNase and was resistant to the enzymes, confirming that the compound was replicative form poliovirus RNA.

A low molecular weight, virus-induced RNA of 4 and unknown function is also synthesized independently of polyinosinic-polycytidylic acid.

The significance of the differential inhibition in the synthesis of the compounds presumed to be poliovirus RNA and replicative form poliovirus RNA, and the fact that the capacity of polyinosinic-polycytidylic acid to inhibit the synthesis of infectious RNA is insensitive to actinomycin, is discussed.

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1971-02-01
2022-01-25
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References

  1. Bausek G. H., Merigan T. C. 1969; Cell interaction with a synthetic polynucleotide and interferon production in vitro. Virology 39:491
    [Google Scholar]
  2. Bishop D. H. I., Claybrook J. R., Spiegelman S. 1967; Electrophoretic separation of viral nucleic acids on polyacrylamide gels. Journal of Molecular Biology 26:373
    [Google Scholar]
  3. Cartwright K. L., Burke D. C. 1970; Virus nucleic acids formed in chick embryo cells infected with Semliki Forest virus. Journal of General Virology 6:231
    [Google Scholar]
  4. Field A. K., Tytell A. A., Lampson G. P., Hilleman M. R. 1967; Inducers of interferon and host resistance. II. Multistranded synthetic polynucleotide complexes. Proceedings of the National Academy of Sciences of the United States of America 58:1004
    [Google Scholar]
  5. Field A. K., Tytell A. A., Lampson G. P., Hilleman M. R. 1968; Inducers of interferon and host resistance. V. In vitro studies. Proceedings of the National Academy of Sciences of the United States of America 61:340
    [Google Scholar]
  6. Finkelstein M. S., Bausek G. H., Merigan T. C. 1968; Interferon inducers in vitro: Difference in sensitivity to inhibitors of RNA and protein synthesis. Science, New York 161:465
    [Google Scholar]
  7. Flikke M., Christiansen E., Lahelle O. 1965; Intracellular distribution of poliovirus-induced RNA-dependent RNA polymerase (replicase) in Detroit-6 cells during the replication cycle. Biochemical and Biophysical Research Communications 18:288
    [Google Scholar]
  8. Flikke M., Kjeldsberg E., Lahelle O. 1970; Interference with cell viability and poliovirus multiplication by polyinosinic-polycytidylic acid. Journal of General Virology 9:111
    [Google Scholar]
  9. Friedman R. M., Sonnabend J. A. 1964; Inhibition of interferon action by p-fluorophenylalanine. Nature, London 203:366
    [Google Scholar]
  10. Loening U. E. F. 1967; The fractionation of high-molecular-weight ribonucleic acids by polyacrylamide gel electrophoresis. Biochemical Journal 102:251
    [Google Scholar]
  11. Marcus P. L., Salb J. M. 1966; Molecular basis of interferon action: Inhibition of viral RNA translation. Virology 30:502
    [Google Scholar]
  12. Penman S. 1966; RNA metabolism in HeLa cell nucleus. Journal of Molecular Biology 17:117
    [Google Scholar]
  13. Scherrer K., Darnell J. E. 1962; Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochemical and Biophysical Research Communications 7:486
    [Google Scholar]
  14. Shatkin A. J. 1965; Actinomycin and differential synthesis of reovirus and L cell RNA. Biochemical and Biophysical Research Communications 19:506
    [Google Scholar]
  15. Vilcek J., Ng MUN H., Friedman-Kien A. B., Krawciw T. 1968; Induction of interferon synthesis by synthetic double-stranded polynucleotides. Journal of Virology 2:648
    [Google Scholar]
  16. Wagner E. K., Katz L., Penman S. 1967; The possibility of aggregation of ribosomal RNA during hot phenol-SDS-deproteinization. Biochemical and Biophysical Research Communications 28152
    [Google Scholar]
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