Cells infected with Newcastle disease virus contained significant amounts of base-paired RNA. Base pairing in this RNA was suggested by its resistance to ribonuclease and by the sharp thermal transition from ribonuclease resistance to sensitivity at 83° in 0.01 m-NaCl. The base-paired RNA sediments heterogeneously with a peak at 35 s.
Preferential labelling of the base-paired RNA after short periods of incubation with [3H]uridine suggests that it may serve as an intermediate in the synthesis of single-stranded virus-specific RNA. The kinetics of [3H]uridine incorporation into the various species of virus-specific RNA are discussed.
BarryR. D.,
BukrinskayaA. G.1968; The nucleic acid of Sendai virus and ribonucleic acid synthesis in cells infected by sendai virus. Journal of General Virology 2:71
BlairC. D.,
RobinsonW. S.1968; Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology 35:537
BrattM. A.,
RubinH.1967; Specific interference among strains of Newcastle disease virus. I. Demonstration and measurement of the interference. Virology 33:598
DuesbergP. H.,
RobinsonW. S.1965; Isolation of the nucleic acid of Newcastle disease virus (NDV). Proceedings of the National Academy of Sciences of the United States of America 54:794
GeiduschekE. P.,
MoohrJ. W.,
WeissS. B.1962; The secondary structure of complementary RNA. Proceedings of the National Academy of Sciences of the United States of America 48:1078
GranoffA.,
KingsburyD. W.1964; Effect of actinomycin D on the replication of Newcastle disease and influenza viruses. In CIBA Foundation Symposium on Cellular Biology of Myxovirus Infections p 96 Ed. by
WolstenholmeG. E. W.,
KnightJ.
London: Churchill;
KingsburyD. W.1966a; Newcastle disease virus RNA. I. Isolation and preliminary characterization of RNA from virus particles. Journal of Molecular Biology 18:195
KingsburyD. W.1966b; Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. Journal of Molecular Biology 18:204
KingsburyD. W.1967; Newcastle disease virus complementary RNA: Its relationship to the viral genome and its accumulation in the presence or absence of actinomycin D. Virology 33:227
SchafferF. L.,
HackettA. J.,
SoergelM. E.1968; Vesicular stomatitis virus RNA: Complementarity between infected cell RNA and RNAs from infectious and auto-interfering viral fractions. Biochemical and Biophysical Research Communications 31:685
StampferM.,
BaltimoreD.,
HuangA. S.1969; Ribonucleic acid synthesis of vesicular stomatitis virus. I. Species of ribonucleic acid found in Chinese Hamster Ovary Cells infected with plaque-forming and defective particles. Journal of Virology 4:154
WeissmannC.,
BorstP.,
BordonR. H.,
BilleterM. A.,
OchoaS.1964; Replication of viral RNA. III. Double stranded replicative form of MS 2 phage RNA. Proceedings of the National Academy of Sciences of the United States of America 51:682