RT Journal Article SR Electronic(1) A1 Winters, A. L. A1 Consigli, R. A.YR 1971 T1 Effects of Arginine Deprivation on Polyoma Virus Infection of Mouse Embryo Cultures JF Journal of General Virology, VO 10 IS 1 SP 53 OP 63 DO https://doi.org/10.1099/0022-1317-10-1-53 PB Microbiology Society, SN 1465-2099, AB SUMMARY Polyoma virus demonstrated a requirement for all of the individual amino acids of Eagle’s minimal essential medium; however, arginine deprivation inhibited virus synthesis to the greatest extent. Early recovery of virus synthesis upon addition of arginine to an arginine-deprived infection indicated that arginine deprivation possibly inhibited a late step of virus synthesis. Arginine deprivation inhibited polyoma virus DNA synthesis by 60%, whereas plaque-forming activity was inhibited approximately 90% during the same period. Arginine deprivation did not affect the number of cells synthesizing structural antigens; however, the encapsidation of virus DNA, dependent upon the synthesis of all structural proteins necessary for virus maturation, was inhibited approximately 90%. A portion of the particles synthesized in arginine-deprived cultures was susceptible to nuclease digestion suggesting incomplete assembly., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-10-1-53