Polyoma virus demonstrated a requirement for all of the individual amino acids of Eagle's minimal essential medium; however, arginine deprivation inhibited virus synthesis to the greatest extent. Early recovery of virus synthesis upon addition of arginine to an arginine-deprived infection indicated that arginine deprivation possibly inhibited a late step of virus synthesis. Arginine deprivation inhibited polyoma virus DNA synthesis by 60%, whereas plaque-forming activity was inhibited approximately 90% during the same period. Arginine deprivation did not affect the number of cells synthesizing structural antigens; however, the encapsidation of virus DNA, dependent upon the synthesis of all structural proteins necessary for virus maturation, was inhibited approximately 90%. A portion of the particles synthesized in arginine-deprived cultures was susceptible to nuclease digestion suggesting incomplete assembly.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error