1887

Abstract

SUMMARY

Injection of mice with mouse brain suspensions of lymphocytic choriomeningitis virus produced a non-specific virus inhibitor which required 48 to 72 hr to act in monkey kidney and HeLa cell cultures. Like interferon it was active against numerous RNA viruses, was non-sedimentable, was unaffected by antiserum to lymphocytic choriomeningitis virus, and showed a dose—response effect. However, unlike interferon it was acid-labile, crossed species barriers, and was eliminated by changing the medium at the time of virus challenge. Mouse brain preparations freed of detectable virus by ultracentrifugation had properties similar to preparations containing lymphocytic choriomeningitis virus. Furthermore, tissue culture fluids containing lymphocytic choriomeningitis virus contained no inhibitor. Tissues of mice infected with the strain of lymphocytic choriomeningitis virus also contained no inhibitor. Hence, this inhibitor appears to represent a new class of broad spectrum virus inhibitor that may be useful for the characterization of lymphocytic choriomeningitis virus strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-10-1-17
1971-01-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/10/1/JV0100010017.html?itemId=/content/journal/jgv/10.1099/0022-1317-10-1-17&mimeType=html&fmt=ahah

References

  1. Baron S. 1966; The biological significance of the interferon system. In Interferons Ed. by Finter N. B. Philadelphia, Pennsylvania: W. B. Saunders Company;
    [Google Scholar]
  2. Bucca M. A. 1964 Viral and Rickettsial Reagents Procedures. National Communicable Disease Centre; Atlanta, Georgia, U.S.A.:
    [Google Scholar]
  3. Camyre K. P., Pfau C. J. 1968; Biophysical and biochemical characterization of lymphocytic choriomeningitis virus. IV. Strain differences.. Journal of Virology 2:161
    [Google Scholar]
  4. Chanock R. M., Hayflick L., Barile M. F. 1962; Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proceedings of the National Academy of Sciences of the United States of America 48:41
    [Google Scholar]
  5. Dalldorf G. 1939; Studies on the sparing effect of lymphocytic choriomeningitis virus on experimental poliomyelitis. I. Effect on the infectivity of monkey tissues.. Journal of Immunology 37:245
    [Google Scholar]
  6. Finter N. B. 1964; A rich source of mouse interferon. Nature, London 204:1114
    [Google Scholar]
  7. Finter N. B. 1966; Interferon assays and standards. In Interferons Ed. by Finter N. B. Philadelphia, Pennsylvania: W. B. Saunders Company;
    [Google Scholar]
  8. Friedman R. M., Rabson A. S., Kirkham W. R. 1963; Variation in interferon production by polyoma virus strains of differing oncogenicity. Proceedings of the Society for Experimental Biology and Medicine 112:347
    [Google Scholar]
  9. Hotchin J. E., Cinits M. 1958; Lymphocytic choriomeningitis infection of mice as a model for the study of latent virus infection. Canadian Journal of Microbiology 4:149
    [Google Scholar]
  10. Jungeblut C. W., Kodza H. 1963; Interference between lymphocytic choriomeningitis virus and the leukemia-transmitting agent of leukemia L2C in guinea pigs.. Archiv für die gesamte Virusforschung 12:552
    [Google Scholar]
  11. Lampson G. P., Tytell A. A., Nemes M. M., Hilleman M. R. 1963; Purification and characterization of chick embryo inteferon. Proceedings of the Society for Experimental Biology and Medicine 112:468
    [Google Scholar]
  12. Lewis V., Clayton D. 1969; Enhanced resistance to arboviruses of mice with persistent lymphocytic choriomeningitis virus infection. Canadian Journal of Microbiology 15:1468
    [Google Scholar]
  13. Lockart R. Z. Jun. 1963; Production of an interferon by L cells infected with western equine encephalomyelitis virus.. Journal of Bacteriology 85:556
    [Google Scholar]
  14. Lockart R. Z. Jun. 1966; Biological properties of interferons; criteria for acceptance of a viral inhibitor as an interferon.. In Interferons. Ed. by Finter. N. B. Philadelphia, Pennsylvania:: W. B. Saunders Company.;
    [Google Scholar]
  15. Marcus P. L., Carver D. H. 1965; Hemadsorption-negative plaque test: new assay for rubella virus revealing a unique interference.. Science New York: 149983
    [Google Scholar]
  16. Marcus P. L., Carver D. H. 1967; Intrinsic interference: a new type of viral interference.. Journal of Virology 1:334
    [Google Scholar]
  17. Marcus P. I., Carver D. H. 1968; Intrinsic interference: a new type of viral interference.. Medical and Applied Virology. Proceedings of the Second International Symposium Ed. by Sanders M., Lennette E. H. St Louis, Missouri: W. H. Green Inc.;
    [Google Scholar]
  18. Reed L. J., Muench H. 1938; Simple method of estimating 50 per cent endpoints. American Journal of Hygiene 27:493
    [Google Scholar]
  19. Ruiz-gomez J., Isaacs A. 1963; Interferon production by different viruses. Virology 19:8
    [Google Scholar]
  20. Traub E. 1961; Interference with Eastern equine encephalomyelitis (EEE) virus in the brains of mice immune to lymphocytic choriomeningitis (LCM).. Archiv für die gesamte Virusforschung 11:419
    [Google Scholar]
  21. Traub E., Resting F. 1963; Experiments on heterologous and homologous interference in LCM-infected cultures of murine lymph node cells. Archiv fiir die gesamte Virusforschung 14:55
    [Google Scholar]
  22. Wagner R. R., Levy A. H., Smith T. J. 1968; Techniques for the study of interferons in animal virus-cell systems. In Methods in Virology Ed. by Maramorosch K., Koprowski H. Vol 4 p. 1 New York: Academic Press;
    [Google Scholar]
  23. Wainwright S., Mims C. A. 1967; Plaque assay for lymphocytic choriomeningitis virus based on hemadsorption interference. Journal of Virology 1:1091
    [Google Scholar]
  24. Wasserman F. E. 1968; Methods for the study of viral inhibitors. In Methods in Virology Ed. by Maramorosch K., Koprowski H. Vol 4 p. 53 New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-10-1-17
Loading
/content/journal/jgv/10.1099/0022-1317-10-1-17
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error