1887

Abstract

The defective genome of murine sarcoma virus (MSV) ( isolate) may be rescued from non-producer hamster tumour cells (HT-1) by co-cultivation of the latter and mouse embryo cells with added helper murine leukaemia viruses (MuLV) (Huebner 1966; Huebner, 1967). Rescue of MSV from HT-1 cells by co-cultivation with MuLV-carrier cells has not, however, been described. This report describes the retrieval of MSV from mixed cultures of HT-1 and various MuLV-carrier cells of mouse and hamster origin. Moreover, it was found that the frequency of success and titres of MSV recovered by co-cultivation of HT-1 and F4, one of the MuLV-carrier mouse cell lines, were much higher than those obtained by the conventional method. We have analysed the factors responsible for such high efficiency of genome rescue.

Among the MuLV-carrier cell lines used, F4 and F5 were derived from two of nine Rauscher pseudotype MSV (MSV(RLV) transformed foci of NIH Swiss mouse embryo cells isolated by trypsinization within a cloning ring.

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/content/journal/jgv/10.1099/0022-1317-10-1-107
1971-01-01
2019-10-19
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-10-1-107
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