1887

Abstract

Summary

Difference in plaque-forming ability in chick embryo cells was shown to be a reliable marker for differentiating herpes virus types 1 and 2 when original or low-passaged isolates of the viruses were used. Type 2 viruses produced large plaques at titres equal to those obtained in rabbit kidney cells, while type 1 viruses failed to produce plaques or produced small plaques at a low plaquing efficiency. Naturally occurring populations of type 1 virus were found to contain a small proportion of virus which produced plaques in chick embryo cells, and this proportion increased with passage of the virus in tissue culture. The plaques produced by type 1 virus in chick embryo cells were morphologically distinct from the plaques produced by type 2 virus. The type 1 variants which plaqued in chick embryo cells could be plaque-purified, and the progeny viruses maintained antigenic and biological similarities to the parent type 1 virus except for the relative resistance to the DNA inhibitor ara-A.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-10-1-1
1971-01-01
2019-10-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-10-1-1
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error