1887

Abstract

SUMMARY

The Pasteur strain of fixed rabies virus was inactivated by most common organic solvents, but survived a single treatment with trifluorotricholoro- ethane. The virus was stable at pH values between 5·0 and 10·0. Inactivation by heat was similar to that of other viruses. The virus was destroyed by trypsin and damaged by phospholipase C but not by ribonuclease; enzyme inhibitors did not preserve the infectivity of mouse-brain suspensions. Viral infectivity was diminished only after repeated freezing and thawing or long exposure to ultrasonic vibration. Equilibrium centrifugation in sucrose solutions gave two peaks of infectivity corresponding to densities of 1·13 g./ cm. and 1·19g./cm.. Chromatographic purification on ECTEOLA-cellulose was unsuccessful. Partially purified virus was readily inactivated by the photodynamic effect of methylene blue. Potent suspensions of virus did not agglutinate red cells of several mammalian and avian species under different conditions of pH and temperature.

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1967-10-01
2021-10-21
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References

  1. Almeida J. D., Waterson A. P. 1966; The position of rabies virus in a morphological classification. Proc. 12th Int. Sym. microbiol. Standard Basel: S. Karger and Co;
    [Google Scholar]
  2. Andrewes C. H., Horstmann D. M. 1949; The susceptibility of viruses to ethyl ether. J. gen. Microbiol 3:290
    [Google Scholar]
  3. Bailly J. 1945; Action de l’extrait de pancreas sur le virus de la rage. Bull. Acad. Med 129:253
    [Google Scholar]
  4. Behrens C. A., Schweiger L. B., Barker J. F., Reeves J. L. 1939; Immunization against rabies using avirulent purified vaccines. J. infect. Dis 64:252
    [Google Scholar]
  5. Bell J. F., Wright J. T., Habel K. 1949; Rabies vaccine freed of the factor causing allergic encephalitis. Proc. Soc. exp. Biol. Med 70:457
    [Google Scholar]
  6. Caspary E. A., Field E. J. 1965; An encephalitogenic protein of human origin; some chemical and biological properties. Ann. N. Y. Acad. Sci 122:82
    [Google Scholar]
  7. Cox H. R., van der Scheer J., Aiston S., Bohnel E. 1947; The purification and concentration of influenza virus by means of alcohol precipitation. J. Immun 56:149
    [Google Scholar]
  8. Crandell R. A., Hillis W. D. 1963; Effect of ether on rabies virus. Nature; Lond: 200715
    [Google Scholar]
  9. Depoux R. 1965; Étude du virus rabique cultivé sur diverse cellules d’origine canine (Souche Louis Pasteur). Annls Inst. Pasteur, Paris 108:566
    [Google Scholar]
  10. Donald H. B., Isaacs A. 1954; Counts of influenza virus particles. J. gen. Microbiol 10:457
    [Google Scholar]
  11. Fagan R. 1952; Ineffectiveness of Aureomycin and Terramycin against rabies street virus in mice. Proc. Soc. exp. Biol. Med 81:213
    [Google Scholar]
  12. Fenje P. 1960; A rabies vaccine from hamster kidney tissue cultures. Preparation and evaluation in animals. Can. J. Microbiol 6:605
    [Google Scholar]
  13. Fuenzalida E. 1964; Antirabies antibody response in man to vaccine made from infected suckling mouse brains. Bull. Wld Hlth Org 30:431
    [Google Scholar]
  14. Galloway I. A. 1934; The fixed virus of rabies: The antigenic value of virus inactivated by the photodynamic action of methylene blue and proflavine. Br. J. exp. Path 15:97
    [Google Scholar]
  15. Gard S., Maaløe G. 1959 In The Viruses 1389 Burnet F. M., Stanley W. M. Ed. by New York: Academic Press;
    [Google Scholar]
  16. Gessler A. E., Bender C. E., Parkinson M. C. 1956; A new and rapid method for isolating viruses by selective fluoro-carbon deproteinisation. Tram. N. Y. Acad. Sci 18:781
    [Google Scholar]
  17. Gispen R., Schmittman G. J. P., Saathof B. 1965; Rabies vaccine derived from suckling rabbit brain. Arch. VirusForsch 15:366
    [Google Scholar]
  18. Habel K. 1964; Advances in rabies research. Ergebn. Mikrobiol. ImmunForsch. exp. Ther 38:39
    [Google Scholar]
  19. Habel K., Bell J. F., Wright J. T. 1949; Benzene inactivated rabies vaccine. Proc. Soc. exp. Biol. Med 70:455
    [Google Scholar]
  20. Hamparian V. V., Hilleman M. R., Ketler A. 1963; Contributions to characterization and classification of animal viruses. Proc. Soc. exp. Biol. Med 112:1040
    [Google Scholar]
  21. Hirano N. 1934; On the resistance of rabies to some ferments. Kitasato Archs exp. Med 11:246
    [Google Scholar]
  22. Jonnesco D. 1936; Action du sue pancreatique (homme) sur la virus rabique. Séanc. Mem. Soc. Biol 123:43
    [Google Scholar]
  23. Kaplan C. 1958; The heat inactivation of vaccinia virus. J. gen. Microbiol 18:58
    [Google Scholar]
  24. Kies M. W., Gordon S., Laatsch R. H., Alvoord E. C.Jun 1962; Cellular localization of allergic encephalomyelitic activity in guinea-pig brain. Proc. IVth Int. Congr. of Neuropath 120 Stuttgart: George Thieme Verlag;
    [Google Scholar]
  25. Kitselman C. H. 1958; The influence of certain antibiotics and acidity on rabies virus. J. Am. vet. med. Ass 122:325
    [Google Scholar]
  26. Laatsch R. H., Kies M. W., Gordon S., Alvoord E. C. 1962; The encephalomyelitic activity of myelin isolated by ultracentrifugation. J. exp. Med 115:777
    [Google Scholar]
  27. Lakao A., Roboz-Einstein E. 1965; Chemical and immunochemical studies with a dialysable encephalitogenic compound from bovine spinal cord. Ann. N. Y. Acad. Sci 122:171
    [Google Scholar]
  28. Lepine P., Pavilanis V., Atanasiu P. 1951; Action de la Terramycine sur les virus rabique. Annls Ins. Pasteur, Paris 80:666
    [Google Scholar]
  29. Marks N., Lajtha A. 1965; Separation of acid and neutral proteinases of brain. Biochem. J 97:74
    [Google Scholar]
  30. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behaviour of enzymes: Application to protein mixtures. J. biol. Chem 236:1372
    [Google Scholar]
  31. Mobest H. 1958; Ein experimentaler Hinwies zur Frage der temperaturempfindlichkeit der virus fixe Pittman-Moore. Arch. Hyg. Bakt 142:581
    [Google Scholar]
  32. Mobest H. 1959; Über den Einfluss verschiedener Verdünnungsmittel auf die Infektiositat von Virus fixe Suspension. Zentbl. Bakt. ParasitKde Abt. I, Orig 174:165
    [Google Scholar]
  33. Neurath A. R., Wiktor T. J., Koprowski H. 1966; Density gradient centrifugation studies on rabies virus. J. Bact 92:102
    [Google Scholar]
  34. Okada J. 1953; Studies on the inactivation methods of the virus for preparation of rabies vaccine. Jap. J. med. Sci. Biol 6:577
    [Google Scholar]
  35. Ott G. L., Heyke B. 1961; Preliminary trials of a new tissue culture rabies vaccine. Vet. Med 57:158
    [Google Scholar]
  36. Philipson L., Kaufman M. 1964; The efficiency of ribonuclease inhibitors tested with viral nucleic acid as substrate. Biochim. biophys. Acta 80:151
    [Google Scholar]
  37. Pinteric L., Fenje P., Almeida J. D. 1963; The visualization of rabies virus in mouse brain. Virology 20:208
    [Google Scholar]
  38. Reed L. J., Muench H. 1938; A simple method for estimating fifty per cent endpoints. Am. J. Hyg 27:493
    [Google Scholar]
  39. Robertson D. D., Blight R., Lumsden C. E. 1962; Dialysable peptide as the causative factor in experimental allergic encephalomyelitis. Nature; Lond: 1961005
    [Google Scholar]
  40. Roth J. S. 1953; Ribonuclease: II. Activators and inhibitors for ribonuclease. Archs Biochem. Biophys 44:265
    [Google Scholar]
  41. Sawai Y., Nakino M. 1954; Studies on the purification of rabies virus by the methanol precipitation. Jap. J. exp. Med 24:229
    [Google Scholar]
  42. Shortt H. E., Brooks A. G. 1934; Photodynamic action of methylene blue on fixed virus of rabies (Paris strain). Indian J. Med. Res 21:581
    [Google Scholar]
  43. Sikes R. K., Larghi O. P., Simpson C. P., Winkler W. G. 1966; Physical and chemical properties of rabies virus. Proc. 12th Int. Sym. microbiol. Standard Basel: S. Karger and Co;
    [Google Scholar]
  44. Simpson R. W., Hauser R. E. 1966; Influence of lipids on the viral Phenotype 1. Interaction of myxoviruses and their lipid constituents with phospholipases. Virology 30:684
    [Google Scholar]
  45. Svet-Moldavsky G. J., Svet-Moldavskaya I. A., Kiseleva I. S. 1960; Preparation of allergene-free vaccines against transmissable encephalitides and rabies from the brain of suckling mice and rats. Acta Virol., Prague 4:320
    [Google Scholar]
  46. Tagaya I., Ozawa Y., Kondo A. 1953; Studies on the purification of rabies virus. 1. Application of methanol precipitation and two other methods. Yokohama med. Bull 4:78
    [Google Scholar]
  47. Thomas J. B., Ricker A. S., Baer G. M., Sikes R. K. 1965; Purification of fixed rabies virus. Virology 25:271
    [Google Scholar]
  48. Turner G. S., Kaplan C. 1965; Observations on photodynamic inactivation of vaccinia virus and its effect on immunogenicity. J. Hyg., Camb 63:395
    [Google Scholar]
  49. van Rooyen C. E., RHODES A. J. In Virus Diseases of Man p. 833 New York: Nelson and Sons;
    [Google Scholar]
  50. Wiktor T. J., Fernandes M. V., Koprowski H. 1964; Cultivation of rabies virus in human diploid cell strain W.I. 38. J. Immun 93:353
    [Google Scholar]
  51. Woese C. 1960; Thermal inactivation of animal viruses. Ann. N.Y. Acad. Sci 83:741
    [Google Scholar]
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