1887

Abstract

SUMMARY

When purified preparations of adenovirus type 5 were heated the icosa- hedral capsid of the virus was ruptured at the twelve vertices, allowing the viral DNA to become accessible to the action of DNAse. The viral DNA could then be substantially separated from protein by digestion with trypsin or pronase. An analysis of the distribution of viral DNA by radioactivity and the various capsid complement-fixing antigens after density gradient centrifugation of heated virus suggested the presence of some other hitherto undescribed protein within the capsid.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-1-4-509
1967-10-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/1/4/JV0010040509.html?itemId=/content/journal/jgv/10.1099/0022-1317-1-4-509&mimeType=html&fmt=ahah

References

  1. Chaproniere-Rickenberg D. M., Mahler H. R., Fraser D. 1964; The interaction of DNA and internal protein from coliphage T2. Virology 23:96
    [Google Scholar]
  2. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis virus. J. exp. Med 99:167
    [Google Scholar]
  3. Easterbrook K. B. 1966; Controlled degradation of vaccinia virions in vitro: an electron microscopic study. J. ultrastruct. Res 14:484
    [Google Scholar]
  4. Ginsberg H. S., Pereira H. G., Valentine R. C., Wilcox W. C. 1966; A proposed terminology for the adenovirus antigens and virion morphological subunits. Virology 28:782
    [Google Scholar]
  5. Green M. 1962; Studies on the biosynthesis of viral DNA. Cold Spring. Harb. Symp. quant. Biol 27:219
    [Google Scholar]
  6. Green M., Piña M. 1964; Biochemical studies on adenovirus multiplication. VI. Properties of highly purified tumorogenic human adenoviruses and their DNA’s. Proc. natn. acad. Sci. U.S.A 51:1251
    [Google Scholar]
  7. Green M., Piña M., Kimes R. C. 1967; Biochemical studies on adenovirus multiplication. XII. Plaquing efficiencies of purified human adenoviruses. Virology 31:562
    [Google Scholar]
  8. Hershey A. D. 1955; An upper limit to the protein content of the germinal substance of bacteriophage T2. Virology 1:108
    [Google Scholar]
  9. Horne R. W., Brenner S., Waterson A. P., Wildy P. 1959; The icosahedral form of an adenovirus. J. molec. Biol 1:84
    [Google Scholar]
  10. Ifft J. B., Voet D. H., Vinograd J. 1961; The determination of density distributions and density gradients in binary solutions at equilibrium in the ultracentrifuge. J. phys. Chem 65:1138
    [Google Scholar]
  11. Levine L., Barlow J. L., Van Vunakis H. 1958; An internal protein in T2 and T4 bacteriophages. Virology 6:702
    [Google Scholar]
  12. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  13. Minagawa T. 1961; Some characteristics of the internal protein of phage T2. Virology 13:515
    [Google Scholar]
  14. Pereira H. G., Allison A. C., Balfour B. 1959; Multiplication of adenovirus type 5 studied by infectivity titration and by the fluorescent antibody technique. Virology 7:300
    [Google Scholar]
  15. Russell W. C., Crawford L. V. 1964; Properties of the nucleic acids from some herpes group viruses. Virology 22:288
    [Google Scholar]
  16. Russell W. C., Watson D. H., Wildy P. 1963; Preliminary chemical studies on herpes virus. Biochem. J 87:26p
    [Google Scholar]
  17. Russell W. C., Hayashi K., Sanderson P. J., Pereira H. G. 1967; Adenovirus antigens-a study of their properties and sequential development in infection. J. gen. Virol 1:495
    [Google Scholar]
  18. Smith K. O., Gehle W. O., Trousdale M. D. 1965; Architecture of the adenovirus capsid. J. Bact 90:254
    [Google Scholar]
  19. Valentine R. C., Pereira H. G. 1965; Antigens and structure of the adenovirus. J. molec. Biol 13:13
    [Google Scholar]
  20. Wildy P., Russell W. C., Horne R. W. 1960; Morphology of herpes virus. Virology 12:204
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-1-4-509
Loading
/content/journal/jgv/10.1099/0022-1317-1-4-509
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error