Transformation of Hamster Cells by Adenovirus Type 12 Free

Abstract

SUMMARY

Hamster brain and kidney cells used as secondary cultures were transformed by adenovirus type 12. Transformed brain cells established in serial culture contained adenovirus tumour antigen and produced tumours when injected into hamsters. Conditions under which transformation may be assayed were determined. In such an assay up to 1/20,000 infected cells gave rise to transformed foci. At high virus input the rate of transformation was decreased probably owing to development of cytopathic effect.

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/content/journal/jgv/10.1099/0022-1317-1-4-405
1967-10-01
2024-03-29
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References

  1. Coons A. H. 1958 In General Cytochemical Methods 1 p. 399 Danielli J. F. Ed. by New York: Academic Press Inc;
    [Google Scholar]
  2. Freeman A. E., Black P. H., Wolford R. G., Huebner R. J. 1966a; Effect of calcium on the adenovirus-type 12-rat embryo transformation system. Fedn Proc. Fedn Am. Socs exp. Biol 25:375
    [Google Scholar]
  3. Freeman A. E., Calisher C. H., Price P. J., Turner H. C., Huebner R. J. 1966b; Calcium sensitivity of cell cultures derived from adenovirus-induced tumours. Proc. Soc. exp. Biol. Med 12a:835
    [Google Scholar]
  4. Huebner R. J., Pereira H. G., Allison A. C., Hollinshead A. C., Turner H. C. 1964; Production of type-specific C antigen in virus-free hamster tumour cells induced by adenovirus type 12. Proc. natn. Acad. Sci. U.S.A 51:432
    [Google Scholar]
  5. Levinthal J. D., Petersen W. 1965; In vitro transformation and immunofluorescence with human adenovirus type 12 in rat and rabbit kidney cells. Fedn Proc. Fedn Am. Socs exp. Biol 24:174
    [Google Scholar]
  6. Macpherson I. A., Montagnier L. 1964; Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology 23:291
    [Google Scholar]
  7. Macpherson I. A., Stoker M. G. P. 1962; Polyoma transformation of hamster cell clones-an investigation of genetic factors affecting cell competence. Virology 16:147
    [Google Scholar]
  8. Mcbride W. D., Wiener A. 1964; In vitro transformation of hamster kidney cells by human adenovirus type 12. Proc. Soc. exp. Biol. Med 115:870
    [Google Scholar]
  9. Pereira H. G. 1958; A protein factor responsible for the early cytopathic effect of adenoviruses. Virology 6:601
    [Google Scholar]
  10. Pereira M. S., Maccallum F. O. 1964; Infection with adenovirus type 12. Lancet 1:198
    [Google Scholar]
  11. Pope J. H., Rowe W. P. 1964; Immunofluorescent studies of adenovirus 12 tumours and of cells transformed or infected by adenoviruses. J. exp. Med 120:577
    [Google Scholar]
  12. Stoker M. G. P. 1964; Regulation of growth and orientation in hamster cells transformed by polyoma virus. Virology 24:165
    [Google Scholar]
  13. Stoker M. G. P., Abel P. 1962; Conditions affecting transformation by polyoma virus. Cold Spring Harb. Symp. quant. Biol 27:375
    [Google Scholar]
  14. Stoker M. G. P., Macpherson I. A. 1961; Studies on transformation of hamster cells by polyoma virus in vitro . Virology 14:359
    [Google Scholar]
  15. Watson D. H., Russell W. C., Wildy P. 1963; Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique. Virology 19:250
    [Google Scholar]
  16. Yamane I., Kusano T. 1967; In vitro transformation of cells of hamster brain by adenovirus type 12. Nature; Lond: 213187
    [Google Scholar]
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