1887

Abstract

Summary

Human adenovirus types 1, 2, 3, 5, 6, 7, 12, 14, 16, and 21 failed to replicate beyond input titres in green monkey kidney (GMK) cells. Co-infection of the monkey cells with papovavirus SV40 stimulated replication of all the adenoviruses tested. Addition of the defective SV40 genome in the PARA particle carried by an adenovirus type 7 population enabled all the adenoviruses to replicate in the GMK cells. Growth kinetics of the adenoviruses in GMK cells co-infected with SV40 were similar to the kinetics of the virus following addition of PARA. A latent period of 16 to 24 hr was followed by an exponential increase in infectious virus between 24 and 48 hr after inoculation. Synthesis of the PARA component closely paralleled that of the adenoviruses. Both the adenoviruses and PARA remained largely cell-associated throughout the growth cycle. During the replication of the transcapsidants, the titre of the adenovirus was always greater than the titre of the PARA component. Ratios were generally in the order of 500 infectious units of adenovirus per infectious unit of PARA. These results differed from those obtained with the parent PARA-adenovirus type 7 population in which the adenovirus to PARA ratio was approximately 3:1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-1-3-311
1967-07-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/1/3/JV0010030311.html?itemId=/content/journal/jgv/10.1099/0022-1317-1-3-311&mimeType=html&fmt=ahah

References

  1. Beardmore W. B., Havlick M. J., Serafini A., Mclean I. W.Jun 1965; Interrelationship of adenovirus (type 4) and papovavirus (SV-40) in monkey kidney cell cultures. J. Immun 95:422
    [Google Scholar]
  2. Boeyé A., Melnick J. L., Rapp F. 1965; Adenovirus-SV40 ‘hybrids’: Plaque purification into lines in which the determinant for the SV40 tumor antigen is lost or retained. Virology 26:511
    [Google Scholar]
  3. Boeyé A., Melnick J. L., Rapp F. 1966; SV40-adenovirus ‘hybrids’: presence of two genotypes and the requirement of their complementation for viral replication. Virology 28:56
    [Google Scholar]
  4. Butel J. S., Rapp F. 1966a; Replication in simian cells of defective viruses in an SV40-adenovirus ‘hybrid’ population. J. Bact 91:278
    [Google Scholar]
  5. Butel J. S., Rapp F. 1966b; Inactivation and density studies with PARA-adenovirus 7 (SV40-adenovirus ‘hybrid’ population). J. Immun 97:546
    [Google Scholar]
  6. Butel J. S., Melnick J. L., Rapp F. 1966a; Detection of biologically active adenovirions unable to plaque in human cells. J. Bact 92:433
    [Google Scholar]
  7. Butel J. S., Rapp F., Melnick J. L., Rubin B. A. 1966b; A new determinant for replication of adenovirus type 7 in monkey cells and its transfer to adenovirus type 2. Science, N.Y 154:671
    [Google Scholar]
  8. Easton J. M., Hiatt C. W. 1965; Possible incorporation of SV40 genome within capsid proteins of adenovirus 4. Proc. natn. Acad. Sci. U.S.A 54:1100
    [Google Scholar]
  9. Feldman L. A., Butel J. S., Rapp F. 1966; Interaction of a simian papovavirus and adenoviruses. I. Induction of adenovirus tumor antigen during abortive infection of simian cells. J. Bact 91:813
    [Google Scholar]
  10. Feldman L. A., Melnick J. L., Rapp F. 1965; Influence of SV40 genome on the replication of an adenovirus-SV40 ‘hybrid’ population. J. Bact 90:778
    [Google Scholar]
  11. Huebner R. J., Chanock R. M., Rubin B. A., Casey M. J. 1964; Induction by adenovirus type 7 of tumors in hamsters having the antigenic characteristics of SV 40 virus. Proc. natn. Acad. Sci. U.S.A 52:1333
    [Google Scholar]
  12. Lewis A. M.Jun Baum S. G., Prigge K. O., Rowe W. P. 1966a; Occurrence of adenovirus-SV40 hybrids among monkey kidney cell adapted strains of adenovirus. Proc. Soc. exp. Biol. Med 122:214
    [Google Scholar]
  13. Lewis A. M.Jun Prigge K. O., Rowe W. P. 1966b; Studies of adenovirus-SV40 hybrid viruses. IV. An adenovirus type 2 strain carrying the infectious SV40 genome. Proc. natn. Acad. Sci. U.S.A 55:526
    [Google Scholar]
  14. Malmgren R. A., Rabson A. S., Carney P. G., Paul F. J. 1966; Immunofluoresence of green monkey kidney cells infected with adenovirus 12 and with adenovirus 12 plus simian virus 40. J. Bact 91:262
    [Google Scholar]
  15. Morris J. A., Casey M. J., Eddy B. E., Lane W. T., Huebner R. J. 1966; Occurrence of SV40 neoplastic and antigenic information in vaccine strains of adenovirus type 3. Proc. Soc. exp. Biol. Med 122:679
    [Google Scholar]
  16. Piña M., Green M. 1965; Biochemical studies on adenovirus multiplication, IX. Chemical and base composition analysis of 28 human adenoviruses. Proc. natn. Acad. Sci. U.S.A 54:547
    [Google Scholar]
  17. Rabson A. S., O’Conor G. T., Berezesky I. K., Paul F. J. 1964; Enhancement of adenovirus growth in African green monkey kidney cell cultures by SV40. Proc. Soc. exp. Biol. Med 116:187
    [Google Scholar]
  18. Rapp F. 1966; Complementation between defective oncogenic viruses. In Malignant Transformation by Viruses Kirsten W. H. Ed. by pp. 77–94 New York: Springer-Verlag;
    [Google Scholar]
  19. Rapp F., Butel J. S., Feldman L. A., Kitahara T., Melnick J. L. 1965a; Differential effects of inhibitors on the steps leading to the formation of SV40 tumor and virus antigens. J. exp. Med 121:935
    [Google Scholar]
  20. Rapp F., Butel J. S., Melnick J. L. 1965b; SV40-adenovirus ‘hybrid’ populations: Transfer of SV40 determinants from one type of adenovirus to another. Proc. natn. Acad. Sci. U.S.A 54:717
    [Google Scholar]
  21. Rapp F., Butel J. S., Tevethia S. S., Katz M., Melnick J. L. 1966a; Antigenic analysis of tumors and sera from animals inoculated with PARA-adenovirus populations. J. Immun 97:833
    [Google Scholar]
  22. Rapp F., Feldman L. A., Mandel M. 1966b; Synthesis of virus deoxyribonucleic acid during abortive infection of simian cells by human adenoviruses. J. Bact 92:931
    [Google Scholar]
  23. Rapp F., Kitahara T., Butel J. S., Melnick J. L. 1964a; Synthesis of SV40 tumor antigen during replication of simian papovavirus (SV40). Proc. natn. Acad. Sci. U.S.A 52:1138
    [Google Scholar]
  24. Rapp F., Melnick J. L., Butel J. S., Kitahara T. 1964b; The incorporation of SV40 genetic material into adenovirus 7 as measured by intranuclear synthesis of SV40 tumor antigen. Proc. natn. Acad. Sci. U.S.A 52:1348
    [Google Scholar]
  25. Rapp F., Melnick J. L., Levy B. 1967; Correlation of immunology and histopathology of tumours induced by defective SV40-adenovirus hybrids. Am. J. Path in the Press
    [Google Scholar]
  26. Rapp F., Tevethia S. S., Melnick J. L. 1966c; Papovavirus SV40 transplantation immunity conferred by an adenovirus-SV40 hybrid. J. natn. Cancer Inst 36:703
    [Google Scholar]
  27. Reich P. R., Baum S. G., Rose J. A., Rowe W. P., Weissman S. M. 1966; Nucleic acid homology studies of adenovirus type 7-SV40 interactions. Proc. natn. Acad. Sci. U.S.A 55:336
    [Google Scholar]
  28. Rowe W. P. 1965; Studies of adenovirus-SV40 hybrid viruses. III. Transfer of SV40 gene between adenovirus types. Proc. natn. Acad. Sci. U.S.A 54:711
    [Google Scholar]
  29. Rowe W. P., Baum S. G. 1964; Evidence for a possible genetic hybrid between adenovirus type 7 and SV40 viruses. Proc. natn. Acad. Sci. U.S.A 52:1340
    [Google Scholar]
  30. Rowe W. P., Baum S. G. 1965; Studies of adenovirus SV40 hybrid viruses. II. Defectiveness of the hybrid particles. J. exp. Med 122:955
    [Google Scholar]
  31. Rowe W. P., Baum S. G., Pugh W. E., Hoggan M. D. 1965; Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization. J. exp. Med 122:943
    [Google Scholar]
  32. Rowe W. P., Pugh W. E. 1966; Studies of adenovirus-SV40 hybrid viruses. V. Evidence for linkage between adenovirus and SV40 genetic materials. Proc. natn. Acad. Sci. U.S.A 55:1126
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-1-3-311
Loading
/content/journal/jgv/10.1099/0022-1317-1-3-311
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error