1887

Abstract

Summary

When particles of tobacco rattle virus (TRV) were progressively degraded by alkali, detergent or urea a number of particles of specific lengths were produced. Some of the breakdown products had lengths similar to those of the naturally occurring short rods characteristic of various isolates of TRY. Loss of infectivity was associated with increasing heterogeneity of sedimentation of the long TRV particles during density gradient centrifugation. Cleavage of different strains of TRV produced relatively few fragments from 1800 to 1900 Å resembling the naturally occurring shorter rods.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-1-2-211
1967-04-01
2024-11-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/1/2/JV0010020211.html?itemId=/content/journal/jgv/10.1099/0022-1317-1-2-211&mimeType=html&fmt=ahah

References

  1. Buzzell A. 1960; Action of urea on tobacco mosaic virus. J. Am. chem. Soc 82:1636
    [Google Scholar]
  2. Buzzell A. 1962; Action of urea on tobacco mosaic virus. II. The bonds between protein and sub-units. Biophys. J 2:223
    [Google Scholar]
  3. Caspar D. L. D. 1963; Assembly and stability of the tobacco mosaic virus particle. Adv. Prot. Chem 18:37
    [Google Scholar]
  4. Harrington W. F., Schachman H. K. 1956; Studies on the alkaline degradation of tobacco mosaic virus. I. Ultracentrifugal analysis. Archs Biochem. Biophys 65:278
    [Google Scholar]
  5. Harrison B. D., Nixon H. L. 1959; Separation and properties of tobacco rattle virus with different lengths. J. gen. Microbiol 21:569
    [Google Scholar]
  6. Harrison B. D., Woods R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610
    [Google Scholar]
  7. Hart R. G. 1955; Electron-microscope evidence from the localization of RNA in the particles of tobacco mosaic virus. Proc. natn. Acad. Sci. U.S.A 41:261
    [Google Scholar]
  8. Incardona N. L., Kaesberg P. 1964; A pH-induced structural change in bromegrass mosaic virus. Biophys. J 4:11
    [Google Scholar]
  9. Lister R. M. 1966; Possible relationships of virus-specific products of tobacco rattle virus infections. Virology 28:350
    [Google Scholar]
  10. Miller G. L., Golder R. H. 1950; Buffers of pH 2 to 12 for use in electrophoresis. Archs Biochem 29:420
    [Google Scholar]
  11. Semancik J. S. 1966; Purification and properties of two isolates of tobacco rattle virus from pepper in California. Phytopathology in the Press
    [Google Scholar]
  12. Semancik J. S., Kajiyama M. R. 1967; Comparative studies on two strains of tobacco rattle virus. J. gen. Virol 1:153
    [Google Scholar]
  13. Symons R. H., Rees M. W., Short M. N., Markham R. 1963; Relationships between the ribonucleic acid and protein of some plant viruses. J. molec. Biol 6:1
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-1-2-211
Loading
/content/journal/jgv/10.1099/0022-1317-1-2-211
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error