1887

Abstract

Summary

The infective process of a filamentous bacteriophage was studied in the electron microscope using thin sections of cells of infected at a high multiplicity. Marked intracellular changes beginning after about 20 min. consisted of multiple layer membraneous structures and mesosomes formed by the invagination of the cell wall and the protoplasmic membrane.At the same time many particles were extruded and appeared in transverse section as small circles with a black dot, believed to be the nucleic acid core. It was difficult to see particles within the cell or being extruded from it, but some evidence suggested that bacteriophage synthesis was occurring near the cell wall. There was no abrupt lysis; the cell contents slowly leaked out leaving an intact cell envelope.

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1967-04-01
2022-01-21
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References

  1. Bishop D. H. L., Bradley D. E. 1965; 32P base-ratio determination of six RNA bacteriophages specific to Escherichia coli . Biochem. J 95:82
    [Google Scholar]
  2. Bradley D. E. 1964; The structure of some bacteriophages associated with male strains of Escherichia coli . J. gen. Microbiol 35:471
    [Google Scholar]
  3. Bradley D. E. 1966; The structure and infective process of a Pseudomonas aeruginosa bacteriophage containing ribonucleic acid. J. gen. Microbiol 45:83
    [Google Scholar]
  4. Brinton C. C. 1965; The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in Gram-negative bacteria. Trans. N. Y. Acad. Sci 27:1003
    [Google Scholar]
  5. Brinton C. C., Gemski P., Carnahan J. 1964; A new type of bacterial pilus genetically controlled by the fertility factor of E. coli K12, and its role in chromosome transfer. Proc. natn. Acad. Sci. U.S.A 52:776
    [Google Scholar]
  6. Caro L. G., Schnös M. 1966; The attachment of the male-specific bacteriophage f 1 to sensitive strains of Escherichia coli . Proc. natn. Acad. Sci. U.S.A 56:126
    [Google Scholar]
  7. Cohen-Bazire G., Kunisawa R., Poindexter J. S. 1966; The internal membranes of Caulobacter crescentus . J. gen. Microbiol 42:301
    [Google Scholar]
  8. Crawford E. M., Gesteland R. F. 1964; The adsorption of bacteriophage R17. Virology 22:166
    [Google Scholar]
  9. Franklin R. M., Granboulan N. 1966; Ultrastructure of Escherichia coli cells infected with bacteriophage R17. J. Bact 91:834
    [Google Scholar]
  10. Granboulan N., Franklin R. M. 1966; High resolution autoradiography of Escherichia coli cells infected with bacteriophage R17. J. Bact 91:849
    [Google Scholar]
  11. Hoffmann-Berling H., Mazé R. 1964; Release of male-specific bacteriophages from surviving host bacteria. Virology 22:305
    [Google Scholar]
  12. Hofschneider P. H., Preuss A. 1963; M13 bacteriophage liberation from intact bacteria as revealed by the electron microscope. J. molec. Biol 7:450
    [Google Scholar]
  13. Kay J. J., Chapman G. B. 1963; Cytological aspects of antimicrobial antibiosis. III. Cytologically distinguishable stages in antibiotic action of colistin sulfate on Escherichia coli . J. Bact 86:536
    [Google Scholar]
  14. Kellenberger E., Séchaud J., Ryter A. 1959; Electron microscopical studies of phage multi-plication IV. The establishment of the DNA-pool of vegetative phage and the maturation of phage particles. Virology 8:478
    [Google Scholar]
  15. Kushnarev V. M., Pereverzev N. A. 1964; The membranes in Escherichia coli cells. J. Ultrastruct. Res 10:610
    [Google Scholar]
  16. Loeb T., Zinder N. 1961; A bacteriophage containing RNA. Proc. natn. Acad. Sci. U.S.A 47:286
    [Google Scholar]
  17. Marvin D. A. 1966; X-ray diffraction and electron microscope studies on the structure of the small filamentous bacteriophage fd. J. molec. Biol 15:8
    [Google Scholar]
  18. Marvin D. A., Hoffmann-Berling H. 1963; Physical and chemical properties of two small bacteriophages. Nature; Lond: 197517
    [Google Scholar]
  19. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol 17:208
    [Google Scholar]
  20. Sabatini D. D., Bensch K., Barrnett R. J. 1963; The preservation of cellular ultrastructure and enzymic activity by aldehyde fixation. J. Cell Biol 17:19
    [Google Scholar]
  21. Schwartz F. M., Zinder N. 1963; Crystalline aggregates in bacterial cells infected with RNA bacteriophage f2. Virology 21:276
    [Google Scholar]
  22. Steed P., Murray R. G. E. 1966; The cell wall and cell division of gram-negative bacteria. Can. J. Microbiol 12:263
    [Google Scholar]
  23. Tickhonenko A. S., Bespalova I. A., Krivisky A. S. 1964; Electron microscopy studies of RNA phage MS 2 and its reproduction in bacterial cells. Proc. Eur. Reg. Conf. on Electron Micro-scopy, Prague1964 B:545
    [Google Scholar]
  24. Tzagoloff H., Pratt D. 1964; The initial steps in infection with coliphage M13. Virology 24:372
    [Google Scholar]
  25. Vanderwinkel E., Murray R. G. E. 1962; Organelles intracytoplasmiques bactériens et site d’activité oxydoréductrice. J. Ultrastruct. Res 7:185
    [Google Scholar]
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