1887

Journal of General Virology header logo

Volume 1, Issue 1

Comparative Growth and Selection of Small Plaque and Large Plaque Encephalomyocarditis Virus in the Absence of Inhibitors from Agar Free

Abstract

Summary

During multicycle growth of clones of encephalomyocarditis (EMC) virus forming large plaques (EMC/r), small-plaque-forming virus (EMC/r) appeared and was always selected by conditions in the growth medium to become the predominant plaque type. EMC/r arose but was never selected by conditions in the growth medium to predominance in virus pools initiated by EMC/r virus clones. Comparison of virus growth showed that EMC/r was produced and released from EMC/r infected L cells earlier and in 3- to 5-fold higher yields than was EMC/r from L cells infected with EMC/r. Thus, when separately infected cells are considered, selection strongly favoured EMC/r progeny.

In mixed infection of L cells with EMC/r and EMC/r, it was shown that:

  • (1)  Selection favouring the EMC/r virus was reduced as the exposed multiplicity/cell of EMC/r virus increased from 0·01 to 5·0 p.f.u./cell.
  • (2)  At 1·0 p.f.u./cell EMC/r and 5·0 p.f.u./cell of EMC/r less virus of both types was formed in 8 hr than in controls with each virus alone at the same multiplicity.
  • (3)  Eight hr after infection more EMC/r was produced and/or released at 0·01 or 0·1 p.f.u./cell EMC/r and 1·0 or 5·0 p.f.u./cell of EMC/r, than in control cultures without EMC/r virus.

‘ Pure ’ ( > 99 %) pools of the EMC/r virus strain containing 10 to 10 p.f.u. were produced consistently by increasing the EMC/r virus pool by growth in consecutive single cycle steps, thus eliminating the selective conditions of multicycle growth.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1967
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-1-1-71
1967-01-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/1/1/JV0010010071.html?itemId=/content/journal/jgv/10.1099/0022-1317-1-1-71&mimeType=html&fmt=ahah

References

  1. Breeze D. C. 1964 Studies on encephalomyocarditis virus Ph.D, Thesis University of Glasgow, Scotland:
     [Google Scholar]
  2. Breeze D. C., Subak-Sharpe H. 1967; The mutability of small plaque-forming encephalomyocarditis virus. J. gen. Virol 1:81
     [Google Scholar]
  3. Breeze D. C., Thorne H. V. 1966; The electrophoretic separation of two plaque-type variants of encephalomyocarditis virus. Nature; Lond: 2101080
     [Google Scholar]
  4. Dulbecco R. 1952; Production of plaques in monolayer tissue cultures by single particles of an animal virus. Proc. natn. Acad. Sci. U.S.A 38:747
     [Google Scholar]
  5. Ledinko N., Hirst G. K. 1961; Mixed infection of HeLa cells with poliovirus types 1 and 2. Virology 14:207
     [Google Scholar]
  6. Liebhaber H., Takemoto K. K. 1963; The basis for the size differences in plaques produced by variants of encephalomyocarditis (EMC) virus. Virology 20:559
     [Google Scholar]
  7. Puck T. T., Marcus P. I. 1955; A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of X-irradiated cells to supply conditioning factors. Proc. natn. Acad. Sci. U.S.A 41:432
     [Google Scholar]
  8. Stoker M., Macpherson I. 1964; Syrian hamster fibroblast cell line BHK21 and its derivatives. Nature; Lond: 2031355
     [Google Scholar]
  9. Takemoto K. K., Liebhaber H. 1961; Virus-polysaccharide interactions. I. An agar polysaccharide determining plaque morphology of EMC virus. Virology 14:456
     [Google Scholar]
  10. Thorne H. V. 1963; Electrophoretic study of the interaction of radioactive poliovirus with components of cultured cells. J. Bact 85:1247
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-1-1-71
Loading
Comparative Growth and Selection of Small Plaque and Large Plaque Encephalomyocarditis Virus in the Absence of Inhibitors from Agar
J. Gen. Virol. 1, 71 (1967); https://doi.org/10.1099/0022-1317-1-1-71
/content/journal/jgv/10.1099/0022-1317-1-1-71
/content/journal/jgv/10.1099/0022-1317-1-1-71
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error