Skip to content
1887

Journal of General Virology header logo Journal of General Virology

Volume 1, Issue 1

Apparent Symbiotic Interaction Between Particles of Tobacco Rattle Virus No Access

Abstract

Summary

SUMMARY

The predominant lengths of tubular particles produced by the CAM isolate of tobacco rattle virus (520 and 1950 A long) and of the PRN isolate (650 to 800 and 1900 A long) were separated by density gradient centrifugation. On their own, short particles of each isolate neither caused lesions in leaves nor multiplied detectably. Long particles of either isolate caused lesions which contained virus nucleic acid, but virus particles were not found in extracts of the lesions; when the lesions were thawed after 3 days storage at −15°, the extracts had little or no infectivity. When short particles were mixed with long particles of the same isolate, the number of lesions produced by the long particles was not affected, but a proportion of these lesions then contained tubular virus particles of both lengths, and also infective material resistant to freezing and thawing. This proportion increased from zero to unity with increasing concentration of short particles in the inoculum; it also increased with increasing susceptibility to infection of the plants used as the of the lesions, but seemed unaffected by the concentration of long particles in the inoculum. With isolate CAM, the lesions containing stable virus particles could be distinguished by their smaller necrotic centres. When short particles were ultraviolet-irradiated, their ability to interact with long ones was abolished, but the interaction was not prevented by removing virus protein from both long and short particles by phenol treatment. No interaction was detected between particles of the two different isolates, which are distantly related serologically, or between their nucleic acids. Each isolate of tobacco rattle virus seems to be a system of two or more pieces of infective nucleic acid interacting specifically in a symbiotic manner.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1967
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-1-1-57
1967-01-01
2025-01-20
Loading full text...

Full text loading...

References

  1. Babos P., Kassanis B. 1962; Unstable variants of tobacco necrosis virus. Virology 18:206
     [Google Scholar]
  2. Bos L., Van der Want J. P. H. 1962; Early browning of pea, a disease caused by a soil- and seed-borne virus. Tijdschr. PlZiekt 68:368
     [Google Scholar]
  3. Brakke M. K. 1964; Nonideal sedimentation and the capacity of sucrose gradient columns for virus in density-gradient centrifugation. Archs Biochem. Biophys 107:388
     [Google Scholar]
  4. Cadman C. H. 1962; Evidence for association of tobacco rattle virus nucleic acid with a cell component. Nature; Lond: 19349
     [Google Scholar]
  5. Cadman C. H., Harrison B. D. 1959; Studies on the properties of soil-bome viruses of the tobacco-rattle type occurring in Scotland. Ann. appl. Biol 47:542
     [Google Scholar]
  6. Fulton R. W. 1962; The effect of dilution on necrotic ringspot virus infectivity and the enhancement of infectivity by noninfective virus. Virology 18:477
     [Google Scholar]
  7. Gibbs A. J., Harrison B. D. 1964; A form of pea early-browning virus found in Britain. Ann. appl. Biol 54:1
     [Google Scholar]
  8. Harrison B. D. 1966; Further studies on a British form of pea early-browning virus. Ann. appl. Biol 57:121
     [Google Scholar]
  9. Harrison B. D., Nixon H. L. 1959a; Separation and properties of particles of tobacco rattle virus with different lengths. J. gen. Microbiol 21:569
     [Google Scholar]
  10. Harrison B. D., Nixon H. L. 1959b; Some properties of infective preparations made by disrupting tobacco rattle virus with phenol. J. gen. Microbiol 21:591
     [Google Scholar]
  11. Harrison B. D., Woods R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610
     [Google Scholar]
  12. Harrison B. D., Nixon H. L., Woods R. D. 1965; Lengths and structure of particles of barley stripe mosaic virus. Virology 26:284
     [Google Scholar]
  13. Kassanis B. 1962; Properties and behaviour of a virus depending for its multiplication on another. J. gen. Microbiol 27:477
     [Google Scholar]
  14. Kassanis B., Nixon H. L. 1961; Activation of one tobacco necrosis virus by another. J. gen. Microbiol 25:459
     [Google Scholar]
  15. Kassanis B., Welkie G. W. 1963; The nature and behavior of unstable variants of tobacco necrosis virus. Virology 21:540
     [Google Scholar]
  16. Lister R. M. 1966; Possible relationships of virus-specific products of tobacco rattle virus infections. Virology 28:350
     [Google Scholar]
  17. Maat D. Z. 1963; Pea early-browning virus and tobacco rattle virus-two different, but serologically related viruses. Neth. J. Plant Path 69:287
     [Google Scholar]
  18. Nixon H. L., Fisher H. L. 1958; An improved spray droplet technique for quantitative electron microscopy. Br. J. appl. Phys 9:68
     [Google Scholar]
  19. Paul H. L., Bode O. 1955; Elektronenmikroskopische Untersuchungen über Kartoffelviren II. Vermessung der Teilchen von drei Stämmen des Rattle-Virus. Phytopath. Z 24:341
     [Google Scholar]
  20. Sänger H. L., Brandenburg E. 1961; Über die Gewinnung von infektiösem Presssaft aus ‘Wintertyp’-Pflanzen des Tabak-Rattle-Virus durch Phenolextraktion. Naturwissenschaften 48:391
     [Google Scholar]
  21. Wood H. A., Bancroft J. B. 1965; Activation of a plant virus by related incomplete nucleo- protein particles. Virology 27:94
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-1-1-57
Loading
Apparent Symbiotic Interaction Between Particles of Tobacco Rattle Virus
J. Gen. Virol. 1, 57 (1967); https://doi.org/10.1099/0022-1317-1-1-57
/content/journal/jgv/10.1099/0022-1317-1-1-57
/content/journal/jgv/10.1099/0022-1317-1-1-57
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error