RT Journal Article SR Electronic(1) A1 MÉcs, E. A1 Sonnabend, J. A. A1 Martin, E. M. A1 Fantes, K. H.YR 1967 T1 The Effect of Interferon on the Synthesis of RNA in Chick Cells Infected with Semliki Forest Virus JF Journal of General Virology, VO 1 IS 1 SP 25 OP 40 DO https://doi.org/10.1099/0022-1317-1-1-25 PB Microbiology Society, SN 1465-2099, AB Summary When cells were pretreated with amounts of interferon which caused a 92 to 99 % reduction in virus production, the yields of infectious RNA were inhibited by 67 to 81 %, while the rate of incorporation of [3H]adenosine into total virus specific RNA was inhibited by only 48 to 65 %. Sucrose gradient analysis of the virus specific RNA synthesized in untreated infected cells showed it to consist of (i) 45 S infectious RNA which is probably the RNA of the mature virus, (ii) 26 S ribonuclease-sensitive RNA, possessing not more than 3 % of the infectivity of 45 S RNA, whose function is not known, but which is the major component early in infection, and (iii) 20 S ribo- nuclease-resistant RNA, which is probably a double-stranded replicative form. The inhibition by interferon of the synthetic rates of these three RNA species was not uniform. 45 S RNA showed the same sensitivity to interferon as did infectious RNA. The synthesis of 20 S ribonuclease-resistant RNA and of 26 S ribonuclease-sensitive RNA were relatively resistant to interferon action., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-1-1-25