Transformation, i.e. heritable morphological and growth changes, of cultured bovine cells induced by bovine papilloma virus has been reported by several authors (1, 2). We report here the finding that suspensions containing polyoma virus which has the ability to cause a transformation in cultured cells of several species of rodents (3–6) can also cause proliferation and various persistent alterations in certain bovine tissue culture cells.

The cellular changes were first recognized in two clones of diploid cells, P-3 and P-6, obtained from cultures of foetal bovine skin (7), after exposure to two different preparations of plaque-purified polyoma virus (Toronto small-plaque strain). The cells were seeded in 50 mm. diameter plastic Petri dishes (2 × 10 cells per dish) and were grown and maintained at 36° in a humidified atmosphere of 5% (v/v) CO in air. The medium consisted of modified Eagle's medium containing additional amino acids and vitamins (8) supplemented with 10% (v/v) unheated foetal calf serum, penicillin (100 u./ml.) and streptomycin (100µg./ml.).


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