1887

Abstract

Summary

High concentrations of glycerol and glucose decreased the rate of photoinactivation of vaccinia virus by methylene blue. These results could not be attributed to changes in the viscosity of the medium or to the impairment of the initial dye sensitization of the virus. Inhibition of photoinactivation by glycerol was not wholly dependent on O concentration. Lyophilization of dye-sensitized virus prevented its subsequent inactivation by exposure to light. These results are interpreted as indicating that water is involved in the photoinactivation process, although its role is as yet obscure.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-1-1-109
1967-01-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/1/1/JV0010010109.html?itemId=/content/journal/jgv/10.1099/0022-1317-1-1-109&mimeType=html&fmt=ahah

References

  1. Dewey D. L. 1960; Effect of glycerine on the X-ray sensitivity of Serratia marcescens . Nature; Lond: 1871008
    [Google Scholar]
  2. Egerton G. S. 1964; Photosensitizing properties of dyes and white pigments. Nature; Lond: 2041153
    [Google Scholar]
  3. Fridovich I., Handler P. 1960; Detection of free radicals in illuminated dye solutions by the initiation of sulphite oxidation. J. biol. Chem 235:1835
    [Google Scholar]
  4. Grossweiner L. I., Zwicker E. F. 1961; Transient measurements of photochemical processes in dyes. 1. The photosensitized oxidation of phenol by eosin and related dyes. J. chem. Phys 34:1411
    [Google Scholar]
  5. Kay R. E., Walwick E. R., Gifford C. K. 1964; Spectral changes in a cationic dye due to interaction with macromolecules. II. Effects of environment and macromolecular structure. J. phys. Chem 68:1907
    [Google Scholar]
  6. Lea D. E. 1962 Actions of Radiations on Living Cells, 2nd ed. London: Cambridge University Press;
    [Google Scholar]
  7. Mcfarlane A. S., Macfarlane M. G., Amies C. R., Eagles G. H. 1939; A physical and chemical examination of vaccinia virus. Br. J. exp. Path 20:485
    [Google Scholar]
  8. Postuma J., Berends W. 1966; Energy transfer in aqueous solution. Biochim. biophys. Acta 112:422
    [Google Scholar]
  9. Simons J. P. 1959; The reactions of electronically excited molecules in solution. Q. Rev. chem. Soc 13:3
    [Google Scholar]
  10. Simpson L. P., Kirby-Smith J. S., Randolph M. L. 1963; Electron spin resonance in photodynamic dyes. Nature; Lond: 199243
    [Google Scholar]
  11. Smadel J. E., Pickels E. G., Shedlovsky T. 1938; Ultracentrifugation studies on the elementary bodies of vaccine virus. II. The influence of sucrose, glycerol and urea solutions on the physical nature of vaccinia virus. J. exp. Med 68:607
    [Google Scholar]
  12. Smith D. E., Santamaria L., Smaller B. 1961; Free radicals in photodynamic systems. In Free Radicals in Biological Systems Blois L. M. S. Jnr Ed. by New York: Academic Press;
    [Google Scholar]
  13. Spikes J. D., Ghiron C. A. 1964 In hysical Processes in Radiation Biology Augenstern L., Mason R., Rosenberg B. Ed. by New York: Academic Press;
    [Google Scholar]
  14. Strauss G., Nickerson W. J. 1961; Photochemical cleavage of water by riboflavin. II. Role of activators. J. Am. chem. Soc 83:3187
    [Google Scholar]
  15. Turner G. S., Kaplan C. 1965; Observations on photo-dynamic inactivation of vaccinia virus and its effect on immunogenicity. J. Hyg., Camb 63:395
    [Google Scholar]
  16. Turner G. S. 1966 Studies on the Inactivation of Virus Infectivity Ph.D, Thesis University of London:
    [Google Scholar]
  17. Wallis C., Melnick J. L. 1964; Irreversible photo-sensitization of viruses. Virology 23:520
    [Google Scholar]
  18. Webb R. B., Powers E. L. 1961; Water, glycerol and oxygen as factors in radiation sensitivity of bacterial spores. Radiat. Res 14:515
    [Google Scholar]
  19. Wood T. H. 1959; Inhibition of cell division. Radiat. Res. Supp 1:332
    [Google Scholar]
  20. Yamamoto N. 1958; Photodynamic inactivation of bacteriophage and its inhibition. J. Bact 75:443
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-1-1-109
Loading
/content/journal/jgv/10.1099/0022-1317-1-1-109
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error