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High concentrations of glycerol and glucose decreased the rate of photoinactivation of vaccinia virus by methylene blue. These results could not be attributed to changes in the viscosity of the medium or to the impairment of the initial dye sensitization of the virus. Inhibition of photoinactivation by glycerol was not wholly dependent on O2 concentration. Lyophilization of dye-sensitized virus prevented its subsequent inactivation by exposure to light. These results are interpreted as indicating that water is involved in the photoinactivation process, although its role is as yet obscure.
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