- Volume 69, Issue 2, 2019

A Gram-stain-negative, strictly aerobic, rod-shaped motile bacterium with a single polar flagellum, designed strain ALS 81T, was isolated from sediment sample collected in the AiLian Bay of Weihai, China. Phylogenetic analysis based on 16SrRNA gene sequence indicated that strain ALS 81T forms a lineage within the family   Alteromonadaceae   that is distinct from the most closely related genera   Agarivorans  (93.9–94.4 % 16S rRNA gene sequence similarity),   Celerinatantimonas   (92.0 %) and   Aliagarivorans   (91.6 %). Optimum growth occurred in the presence of 3–4 % (w/v) NaCl, at pH 8.0 and at 28 °C. The major fatty acids (>10 % of total fatty acids) were iso-C_16 : 0 (27.3 %), anteiso-C_15 : 0 (27.0 %) and anteiso-C_17 : 0 (11.2 %). The predominant polar lipids were phosphatidylethanolamine, glycolipid and four unidentified polar lipids. The DNA G+C content of strain ALS 81T calculated on the basis of the genome sequence is 43.5 mol% and the genome size is 4.5 Mbp. The predominant isoprenoid quinone is ubiquinone 8. On the basis of polyphasic analysis, strain ALS 81T is considered to represent a novel species in a novel genus of the family   Alteromonadaceae  , for which the name Alginatibacterium sediminis gen. nov., sp. nov. is proposed. The type strain of the type species is ALS 81T (=KCTC 62397T=MCCC 1K03481T).

A bacterial strain, designated WCHPs060039T, was isolated from hospital sewage in China. The strain was Gram-stain-negative, obligate aerobic, flagellum-motile and positive for oxidase and catalase. A preliminary analysis of the 16S rRNA gene sequences indicated that strain WCHPs060039T belonged to the genus Pseudomonas and was closely related to members of the Pseudomonas putida group, with the highest similarities to Pseudomonas entomophila L48T (99.5 %), Pseudomonas mosselii CIP 105259T (99.52 %), Pseudomonas taiwanensis BCRC 17751T (99.45 %) and Pseudomonas plecoglossicida NBRC 103162T (99.31 %). Whole genome sequencing of the strain was performed. Phylogenetic analysis based on a set of core gene sequences revealed that the strain was distinct from its closest Pseudomonas species. Average nucleotide identity based on blast and in silico DNA-DNA hybridizationrevealed low genome relatedness to its closest Pseudomonas species (below the recommended thresholds of 95 and 70 %, respectively, for species delineation). The major fatty acids of the strain were 16:0, 17:0 cyclo, summed feature 3 (16:1ω7c/16:1ω6c and 16:1ω6c/16:1ω7c) and summed feature 8 (18:1ω7c). The ability to utilize inositol, sorbitol and d-glucuronic acid could differentiate strain WCHPs060039T from the closely related Pseudomonas species. It is therefore evident that strain WCHPs060039T represents a novel species of the genus Pseudomonas , for which the name Pseudomonas sichuanensis sp. nov. is proposed. The type strain is WCHPs060039T (GDMCC 1.1424T=CNCTC 7662T).

A novel Gram-stain-negative, aerobic, non-motile, coccus-shaped bacterium, designated CFH 90064T, was isolated from a salt lake sediment sample collected from Yuncheng city, Shanxi province, PR China. 16S rRNA gene sequence comparisons and phylogenetic analyses showed that the strain belonged to the genus Paracoccus and clustered with Paracoccus zeaxanthinifaciens R-1512T (98.2 % similarity), Paracoccus homiensis DD-R11T (97.6 % similarity) and Paracoccus fistulariae 22–5T (96.5 % similarity), respectively. Growth of strain CFH 90064T was observed at 10–37 °C, pH 6.0–9.0 and with NaCl concentrations of up to 6.0 % (w/v). Strain CFH 90064T contained Q-10 as the only isoprenoid quinone, and the major fatty acid was C_18 : 1ω7c. Polar lipids of strain CFH 90064T comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified glycolipid, an unidentified aminolipid and an unidentified phospholipid. The genome of strain CFH 90008T was 3.75 Mbp with a DNA G+C content of 65.1 %. Based on the phylogenetic analyses, low average nucleotide identity results, chemotaxonomic characteristics and differential physiological properties, strain CFH 90064T could not be classified into any recognized species of the genus Paracoccus , suggesting that this strain represents a novel species, for which the name Paracoccus halotolerans sp. nov. is proposed. The type strain is CFH 90064T (=CCTCC AB 2016131T=DSM 103234T).

A Gram-stain-negative, motile with flagellum, ovoid- or rod-shaped, aerobic bacterium, was isolated from the phycosphere of the microalga Chlorellavulgaris and designated as strain LMIT002T. The bacterium formed white, circular and smooth colonies on marine agar 2216 after 48 h incubation at 25 °C. On the basis of 16S rRNA gene sequence analysis, strain LMIT002T was found to be affiliated with the family Rhodobacteraceae of the order Rhodobacterales, and formed a distinct group. The 16S rRNA gene sequence similarities between strain LMIT002T and type strains in the family including Poseidonocella pacifica DSM 29316T, Roseobacter litoralis and Rhodovulum sulfidophilum were 95.1, 95.0 and 95.0 %, respectively. Strain LMIT002Tgrew optimally at 25 °C, pH 6.0 and in the presence of 2.0 % (w/v) NaCl. The genomic DNA G+C content was 67.0 mol% (the thermal denaturation method) or 66.9 % (genome sequencing). The sole respiratory quinone was ubiquinone-8, while the major fatty acids were summed feature 8 (C_18 : 1 ω7с/C_18 : 1 ω6с). The draft genome of strain LMIT002T was sequenced and annotated, with the results showing that it had a total size of 4 607 780 bp and comprised 4557 genes. Functional genes encoding the production of vitamin B12, indole-3-acetic acid and transform dimethylsulphoniopropionate were detected. Given its distinct genomic, morphological and physiological differences from previously described type strains, strain LMIT002T is proposed as a representative of a novel genus of the family Rhodobacteraceae , with the name Phycocomes zhengii gen. nov., sp. nov. The type strain is LMIT002T (=KCTC 62390T=CICC 24357T).

A Gram-stain-negative, aerobic, catalase-positive, oxidase-positive, motile by a single polar flagellum, rod-shaped strain, designated DSW25-10T, was isolated from the deep-sea water of the East Sea, Republic of Korea. Strain DSW25-10Tgrew at 4–35 °C (optimum, 20–35 °C), at pH 5.5–9.0 (optimum, pH 7.0) and in the presence of 0–6.0 % NaCl (optimum, 0.5–2 %), and could assimilate valerate, but not assimilate d-mannitol and 3-hydroxy-butyrate. Comparative 16S rRNA gene sequence analysis showed that strain DSW25-10T belongs to the genus Zhongshania in the family Spongiibacteraceae and is most closely related to Zhongshania guokunii ZS6-22T, Zhongshania borealis CL-AS9T, Zhongshania aliphaticivorans SM-2T and Zhongshani antarctica ZS5-23T with a similarities of 97.1, 97.0, 96.7 and 96.6 %, respectively. The G+C content of genomic DNA was 49.1 mol% and the major respiratory quinone was ubiquinone-8. Phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol were identified as the major cellular polar lipids. The predominant cellular fatty acids in strain DSW25-10T were summed feature 3 (comprising C_16 : 1ω7c and/or C_16 : 1ω6c), summed feature 8 (comprising C_18 : 1ω7c and/or C_18 : 1ω6c), C_16 : 0 and C_17 : 1ω8c. The DNA–DNA relatedness values between strain DSW25-10T and related strains were clearly lower than 70 %. On the basis of evidence from a polyphasic analysis, strain DSW25-10T is proposed to represent a novel species, Zhongshania marina sp. nov. The type strain is DSW25-10T (=KCCM 43273T=JCM 17372T).

A Gram-stain-negative, non-spore-forming, aerobic, motile, ovoid or short rod shaped bacterium, designed strain B17T, was isolated from the culture broth of Picochlorum sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain B17T forms a stable cluster with Oceanicolagranulosus MCCC 1A10589T (with the highest 16S rRNA gene similarity of 95.8 %) and Roseisalinusantarcticus DSM 11466T in the family Rhodobacteraceae . The only detected respiratory quinone was Q-10. The major cellular fatty acids were C_18 : 1 ω7c/ω6c, C_17 : 1iso I/anteiso B and C_16 : 0. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid and two unidentified lipids. The genome G+C content was 69 mol%. Characteristics, such as a long lag phase, being motile with single polar flagellum, no aminolipid content, and little or no C_18 : 1ω7c11-methyl and C_19 : 0cyclo ω8c content could significantly distinguish strain B17T from its closely related type strains. Therefore strain B17T is suggested to represent a new species in a new genus, for which Histidinibacterium lentulum gen. nov., sp. nov. is proposed. The type strain B17T (=MCCC 1K03225T=KCTC 52553T).

During a study of yeast diversity in marine waters of the Arabian Gulf surrounding Qatar, 30 strains were isolated that represent a novel species of Kondoa. The isolates were obtained from five locations along the Qatari coast. Species identifications were based on sequence analysis of the internal transcribed spacer (ITS) regions and D1/D2 domains of the large subunit (LSU) rRNA gene. Five strains were selected for further study. The novel species was closely related to Kondoa aeria (CBS 8352T) and Kondoa malvinella (CBS 6082T) but differed from the two species by 5.6 % sequence divergence (16 substitutions and 14 gaps) in the ITS region. On the basis of D1/D2 domains of the LSU rRNA gene, the novel species differed from K . aeria by only >0.35 % sequence divergence (2 substitutions and 0 gaps) and 1.5 % sequence divergence (9 substitutions and 1 gap) to that of K. malvinella. Although the novel species showed a close similarity in the D1/D2 domains to K. aeria (CBS 8352T), based on the significant differences (16 substitutions) in ITS regions and on the basis of physiological and biochemical tests, viz ability to grow at 35 °C, and inability to assimilate, sucrose, raffinose, d,l-lactate, succinate, citrate and nitrite, these strains are considered as novel species of Kondoa. The species name of Kondoa qatarensis f.a., sp. nov. is proposed with specimen 2Y109 as the holotype.

Appendix 9, the orthography appendix of the International Code of Nomenclature of Prokaryotes, provides guidelines for the correct formation of generic names and specific epithets to honour famous microbiologists and other persons connected with natural science. However, no guidelines are given for the correct formation of compound generic names in which the first word element is derived from a personal name. Currently there are 16 such names validly published under the Rules of the Code, but the ways they were formed are inconsistent. We therefore propose an emendation of Appendix 9 to provide uniform guidelines for the formation of such names in the future.