- Volume 67, Issue 11, 2017

A bacterial strain designated LSN-49T was isolated from a brackish river in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain LSN-49T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate accumulating, motile by means of a monopolar flagellum, non-spore forming, straight rods and formed shiny and translucent colonies. Growth occurred at 20–40 °C (optimum, 25–30 °C), at pH 6–10 (optimum, pH 7–8) and with 0–3 % (w/v) NaCl [optimum, 0–1 % (w/v)]. The predominant fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C17 : 1ω8c and C16 : 0. The polar lipid profile consisted of a mixture of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine, (PC), two uncharacterized aminophospholipids (APL1 and APL2), one uncharacterized glycolipid (GL1), four uncharacterized phospholipids (PL1–PL4) and four uncharacterized lipids (L1–L4). The major polyamine was putrescine. The major isoprenoid quinone was Q-8 and the DNA G+C content was 51.0 mol%. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that LSN-49T formed a distinct lineage with respect to closely related genera in the family Pseudoalteromonadaceae . LSN-49T was most closely related to Pseudoalteromonas , Algicola and Psychrosphaera and showed 89.3–92.1 % sequence similarity with members of the family Pseudoalteromonadaceae with validly published names. On the basis of the genotypic and phenotypic data, LSN-49T represents a novel genus and species of the family Pseudoalteromonadaceae , for which the name Salsuginimonas clara gen. nov., sp. nov. is proposed. The type strain is LSN-49T (=BCRC 81005T=LMG 29726T=KCTC 52439T).

A Gram-stain-negative, singly flagellated, aerobic and coccoid, ovoid or rod-shaped bacterium, designated strain JDTF-33T, was isolated from a tidal flat in Jindo, an island of South Korea. Strain JDTF-33T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, demonstrated that strain JDTF-33T belonged to the genus Paraglaciecola , joining the type strain of the species Paraglaciecola aquimarina with 97.9 % sequence similarity. Strain JDTF-33T exhibited 16S rRNA gene sequence similarities of 97.1 and 97.0 % to the type strains of Paraglaciecola arctica and Paraglaciecola psychrophila , respectively, and of 96.1–96.6 % to the type strains of the other species of the genus Paraglaciecola . Strain JDTF-33T showed DNA–DNA relatedness values of 11–24 % to the type strains of the species P. aquimarina , P. arctica and Paraglaciecola psychrophila . Strain JDTF-33T contained Q-8 as the predominant ubiquinone and summed feature 3 (C16 : ω7c and/or C16 : 1ω6c) and C16 : 0 as the major fatty acids. The major polar lipids detected in strain JDTF-33T were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain JDTF-33T was 41.7 mol%. Differential phenotypic properties, together with phylogenetic and genetic data, demonstrate that strain JDTF-33T is separate from species of the genus Paraglaciecola with validly published names . On the basis of the data presented, strain JDTF-33T represents a novel species of the genus Paraglaciecola , for which the name Paraglaciecola aestuariivivens sp. nov. is proposed. The type strain is JDTF-33T (=KCTC 52838T=NBRC 112782T).

A Gram-stain-negative, non-spore-forming, non-motile and coccoid, ovoid or short rod-shaped bacterial strain, designated GHD-05T, was isolated from a tidal flat on the Yellow Sea in South Korea. Strain GHD-05T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain GHD-05T belonged to the genus P aracoccus , clustering with the type strain of P aracoccus aestuariivivens. Strain GHD-05T exhibited 16S rRNA gene sequence similarity values of 97.0–99.1 % to the type strains of P aracoccus aestuariivivens, P aracoccus limosus, P aracoccus laeviglucosivorans and P aracoccus marinus, and of 94.2–96.9 % to the type strains of the other Paracoccus species. Strain GHD-05T contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids detected in strain GHD-05T were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content of strain GHD-05T was 64.1 mol% and its DNA–DNA relatedness values with the type strains of P. aestuariivivens , P aracoccus limosus, P. laeviglucosivorans and P. marinus were 13–32 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GHD-05T is separated from recognized Paracoccus species. On the basis of the data presented here, strain GHD-05T is considered to represent a novel species of the genus Paracoccus , for which the name Paracoccus litorisediminis sp. nov. is proposed. The type strain is GHD-05T (=KCTC 52978T=NBRC 112902T).

A novel acidophilic heterotrophic bacterium, strain Ok2GT, was isolated from a freshwater lake in Japan. Cells of the isolate were Gram-stain-negative and non-motile rods (0.6–0.8×1.0–2.8 µm). Growth was observed at 4–35 °C with an optimum growth temperature of 28 °C. The pH range for growth was 3.0–6.2 with an optimum pH of 4.5. The strain utilized fructose, glucose, sucrose, mannitol, sorbitol, ethanol, benzyl alcohol, pyruvate, yeast extract and tryptone as carbon and energy sources for aerobic growth. DNA G+C content was 62.6 mol%. The major cellular fatty acid and the isoprenoid quinone were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and Q-10, respectively. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain Ok2GT belongs to the genus Acidocella but is distinct from existing species with sequence similarities lower than 97 %. On the basis of these results, strain Ok2GT (=NBRC 112502T=DSM 104037T) is proposed as the type strain of a novel species, Acidocella aquatica sp. nov.

A novel Marinomonas -like, aerobic, Gram-reaction-negative, moderately halophilic, acidophilic, motile by a single polar flagellum, non-spore-forming, rod-shaped bacterium that showed algalytic activity, designated strain Yeongu 1-4T, was isolated from surface seawater of Geoje Island in the South Sea, Republic of Korea. The strain was oxidase-negative and weakly positive for catalase. Growth of this bacterium was observed at temperatures from 4 to 42 °C, at salinities from 0 to 12 % and at pH from 4.5 to 9.0, and it was not able to degrade starch, gelatin, casein or Tween 80. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Yeongu 1-4T was related most closely to Marinomonas spartinae SMJ19T with similarity of 99.3 %. However, levels of DNA–DNA relatedness between strain Yeongu 1-4T and the most closely related species were lower than 70 %, confirming that they represent distinct genomic species. The genomic DNA G+C content of strain Yeongu 1-4T was 44.2 mol%. The organism used Q-8 as the predominant respiratory quinone, and C16 : 1ω7c, C18 : 1ω7c and C16 : 0 as major cellular fatty acids. Based on data from this polyphasic taxonomic study, strain Yeongu 1-4T belongs to a novel species of the genus Marinomonas , within the family Oceanospirillaceae , for which the name Marinomonas algicida is proposed. The type strain is Yeongu 1-4T (=KEMB 9005-327T=MCCC 1K00609T).

A novel marine bacterial strain, designated JAM 119T, was isolated from a tubeworm trophosome in Kagoshima Bay, Japan. Cells were Gram-negative, rod-shaped, non-spore-forming aerobic chemoorganotrophs and motile by means of a single polar flagellum. The isolate grew optimally at 25–27 °C and in the presence of 3 % NaCl. The major respiratory quinone was Q-10. The predominant fatty acid was C18 : 1ω7c. Phosphatidylcholine, phosphatidylglycerol and an unidentified aminolipid were the major polar lipids. On the basis of 16S rRNA gene sequence analysis, the isolated strain was closely affiliated with members of the genus Planktotalea in the class Alphaproteobacteria , and the 16S rRNA gene sequence similarity of the new isolates with the closest related species, Planktotalea frisia SH6-1T, was 97.3 %. The DNA G+C content of the novel strain was 57.0 mol%. Based on differences in taxonomic characteristics, the isolated strain represents a novel species of the genus Planktotalea , for which the name Planktotalea lamellibrachiae sp. nov. (type strain JAM 119T; JCM 31859T=DSMZ 104669T) is proposed.

Cells of strain SW33T, isolated from the seawater of Asan Bay, Republic of Korea, were characterized as Gram-stain-negative, aerobic, rod-shaped, motile and non-spore-forming. Phylogenetic analysis revealed that strain SW33T belonged to the genus Psychrosphaera and clustered distantly with the other genera in the family Pseudoalteromonadaceae in the phylogenetic tree. The 16S rRNA sequences of strain SW33T revealed high similarities to Psychrosphaera saromensis SA4-48T (98.7 %), Psychrosphaera haliotis KDW4T (97.4 %) and Psychrosphaera aestuarii PSC101T (97.3 %). The major fatty acids were C16 : 0 (27.9 %), summed feature 3 (32.2 %) and summed feature 8 (17.2 %). The predominant quinone was Q-8, and the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified amino lipid. The DNA G+C content was 38.3 mol%. The DNA–DNA relatedness values with the three species of Psychrosphaera saromensis KCTC 23240T, Psychrosphaera haliotis KCTC 22500T and Psychrosphaera aestuarii KCTC 32274T were 22, 23 and 18 %, respectively. Based on the phenotypic characteristics and taxonomic analyses, we propose that strain SW33T represents a novel species within the genus Psychrosphaera , for which the name Psychrosphaera aquimarina sp. nov. with the type strain SW33T (=KCTC 52743T=CICC 24249T) is proposed.

A novel strain, designated EM302T, was isolated from a soil sample collected from farmland located in Yesan-gun in the Republic of Korea. The cells were Gram-stain-negative, aerobic, non-motile without flagella and coccobacilli-shaped. Colonies were circular with entire edges, convex, opaque and pink. The strain grew at 15–40 ˚C (optimum, 35 ˚C) and pH 6.0–9.0 (optimum, 7.0). The strain grew in R2A medium without the addition of NaCl. Based on 16S rRNA gene sequences analysis, strain EM302T was found to be phylogenetically related to Roseomonas wooponensis WW53T (96.5 % 16S rRNA gene sequence similarity), Roseomonas arcticisoli MC 3624T (95.2 %), Roseomonas aerilata 5420-30T (94.5 %), Roseomonas pecuniae N75T (94.4 %) and Roseomonas vinacea CPCC 100056T (94.4 %). The major cellular fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and C16 : 0. The predominant respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminolipid. The DNA G+C content of the type strain was 65.7 mol%. On the basis of data presented here, strain EM302T is considered to represent a novel species of the genus Roseomonas , for which the name Roseomonas terricola sp. nov. is proposed. The type strain is EM302T (=KACC 13942T=KCTC 42906T=NBRC 111477T).

A taxonomic study was carried out on strain L54-1-50T, which was isolated from surface seawater of the South China Sea. Cells of strain L54-1-50T were Gram-stain-negative, rod-shaped, oxidase-positive and catalase-positive. Growth was observed at salinities from 0 to 9 % (optimum 2 %, w/v), at pH 6.0–10.0 (optimum 8.0–9.0) and at temperatures from 10 to 45 °C (optimum 25–37 °C), but not at 4 or 50 °C. The 16S rRNA gene sequence analysis indicated that strain L54-1-50T was a member of the genus Oceanibaculum , related to Oceanibaculum indicum P24T (98.8 %) and Oceanibaculum pacificum MC2UP-L3T (97.7 %). The digital DNA–DNA hybridization values between strain L54-1-50T and the two type strains O. indicum P24T and O. pacificum MC2UP-L3T were 35.4±2.5 and 23.7±2.5 %, respectively. The average nucleotide identity values between strain L54-1-50T and two type strains were 79.7 and 88.3 %, respectively. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and C18 : 1 2-OH. The respiratory quinone was Q-10. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, two unidentified phospholipids and three unidentified lipids. The G+C content of the chromosomal DNA was 65.1 mol%. The combined genotypic and phenotypic data showed that strain L54-1-50T represents a novel species of the genus Oceanibaculum , for which the name Oceanibaculum nanhaiense sp. nov. is proposed, with the type strain L54-1-50T (=KCTC 52312T=MCCC 1A05150T).

A Gram-stain-negative, facultative anaerobic, yellow-pigmented, rod-shaped, non-flagellum and non-motile bacterial strain, designed C9-11T, was isolated from mangrove sediment in Zhangzhou, Fujian Province, China. Growth was observed at temperatures from 15 to 37 °C (optimum 30 °C), at salinities from 0 to 4 % (optimum 0.5–2 %) and at pH from 6 to 8 (optimum 7). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain C9-11T was a member of the genus Altererythrobacter . Strain C9-11T was related most closely to Altererythrobacter oceanensis Y2T (96.8 % 16S rRNA gene sequence similarity), followed by Altererythrobacter marinus H32T (96.5 %). The G+C content of the genomic DNA was 63.5 mol%. The dominant fatty acids were C18 : 1ω7c (41.6 %), C17 : 1ω6c (15.4 %), 11-methyl C18 : 1ω7c (8.1 %), summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c 7.4 %) and C16 : 0 (6.2 %). The predominant respiratory quinone was ubiquinone-10 and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence analysis, strain C9-11Tbelongs to genus Altererythrobacte r, but can readily be distinguished from recognized species. Strain C9-11T is proposed as the type strain of a novel species of the genus Altererythrobacte r, for which the name Altererythrobacter mangrovi sp. nov (=MCCC 1K03311T=JCM 32056T) is proposed.

Strain SYSU D8009T was isolated from a desert sample collected from Saudi Arabia. The taxonomic position of the isolate was investigated by a polyphasic approach. The novel isolate was Gram-stain-negative, non-motile, aerobic and non-spore-forming. It was able to grow at 4–45 °C and pH 4.0–8.0, and exhibited NaCl tolerance of up to 1.5 % (w/v). Strain SYSU D8009T shared the closest 16S rRNA gene sequence similarities with members of the family Acetobacteraceae , with a value of less than 96.0 %. In the phylogenetic dendrograms, the strain clustered with the genera Paracraurococcus , Craurococcus and Crenalkalicoccus within the family Acetobacteraceae but with a distinct lineage, thereby demonstrating that the strain should be classified within the family Acetobacteraceae . The respiratory ubiquinone was found to be Q-10. The polar lipids of the strain comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and four unidentified aminolipids. The predominant cellular fatty acids were summed feature 8 (C1 8 : 1ω7c and/or C1 8 : 1ω6c) and C16 : 0. The genomic DNA G+C content of strain SYSU D8009T was determined to be 71.6 mol%. Based on the results of the phylogenetic analyses and differences in the physiological and biochemical characteristics, strain SYSU D8009T merits representation of a novel species of a new genus within the family Acetobacteraceae , for which the name Siccirubricoccus deserti gen. nov., sp. nov. is proposed. The type strain of Siccirubricoccus deserti sp. nov. is SYSU D8009T (=CGMCC 1.15936T=KCTC 62088T).

During a survey of carotenogenic yeasts from cold and oligotrophic environments in Patagonia, several yeasts of the genus Dioszegia (Tremellales, Agaricomycotina) were detected, including three strains that could not be assigned to any known taxa. Analyses of internal transcribed spacer and D1/D2 regions of the large subunit rRNA gene showed these strains are conspecific with several other strains found in the Italian Alps and in Antarctica soil. Phylogenetic analyses showed that 19 of these strains represent a novel yeast species of the genus Dioszegia. The name Dioszegia patagonica sp. nov. is proposed to accommodate these strains and CRUB 1147T (UFMG 195T=CBMAI 1564T=DBVPG 10618T=CBS 14901T; MycoBank MB 819782) was designated as the type strain. This Dioszegia species accumulates biotechnologically valuable compounds such as carotenoid pigments and mycosporines.

In the course of an ongoing study aiming to catalogue the natural yeast biodiversity of Iran, a number of yeasts were isolated from plant material collected from mangrove forests on the shoreline of Qeshm Island. Two strains were identified as members of order Microstromatales. Standard phenotypic, biochemical, physiological characterization and a phylogenetic analyses of the combined 26S rRNA gene (D1/D2 domains) and ITS region sequences showed the conspecificity of these isolates and suggest their placement in the genus Jaminaea, close to Jaminaea lanaiensis and Jaminaea angkoriensis. Here, we describe this species as Jaminiaea pallidilutea sp. nov. with IBRC-M 30284T=DSM 104392T=CBS 14684T as the type strain. The Mycobank accession number is MB 819618.

Nine anamorphic yeast strains isolated from various plant leaves collected in southern China were phylogenetically characterized based on sequences of the internal transcribed spacer (ITS) region, the D1/D2 domains of the large subunit (LSU) rRNA gene, the small subunit (SSU) rRNA gene, the two subunits of the RNA polymerase II gene (RPB1 and RPB2) and the translation elongation factor 1-α (TEF1). Phylogenetic analysis of the combined sequences of the six genes showed that the new strains formed a distinct clade in the class Microbotryomycetes but could not be assigned to any of the existing genera, families or orders of the class. Three separate groups were consistently resolved from the nine new strains based on the combined sequences of the six genes and single gene sequences of ITS, RPB1, RPB2 and TEF1. The results suggest that the nine yeast strains compared represent three novel species in a novel genus. The names Heitmania gen. nov. (MycoBank registration number MB819987), Heitmania litseae sp. nov. (MB820112, type strain CGMCC 2.5697T=CBS 14756T), Heitmania castanopsis sp. nov. (MB819988, CGMCC 2.5698T=CBS 14750T) and Heitmania elacocarpi sp. nov. (MB820113, CGMCC 2.5695T=CBS 14752T) are proposed for the new taxa.

Six yeast strains isolated from olive oil sediments and spoiled olive oils originating from Slovenia and Portugal, respectively, proved to represent an undescribed yeast species based on DNA sequence comparisons. The analysis of gene sequences for internal transcribed spacer regions and the large subunit rRNA gene D1/D2 domain placed the novel species in the genus Kuraishia in a subclade containing Kuraishia capsulata, the type species of the genus. Although the novel species is well separated genetically from the recognized species of the genus, only a minor phenotypic difference differentiating it from Kuraishia capsulata and K. molischiana was observed. Relevant to its isolation source, no lipolytic activity was detected in the strains of the novel species. To accommodate the above-noted strains, Kuraishia mediterranea sp. nov. (holotype: ZIM 2473T; isotype: CBS 15107T; MycoBank no.: MB 822817) is proposed.