- Volume 67, Issue 10, 2017
Volume 67, Issue 10, 2017
- New taxa
-
- Proteobacteria
-
-
Ramlibacter rhizophilus sp. nov., isolated from rhizosphere soil of national flower Mugunghwa from South Korea
More LessA Gram-negative, aerobic, non-motile, long rods or coccoid without flagellum strain, designated THG-YS3.2.7T, was isolated from therhizosphere soil of a Mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10–40 °C (optimum 28–37 °C), at pH 6–8 (optimum 7) and at 0–5 % NaCl (optimum 1 %). The predominant ubiquinone was ubiquinone 8 (Q-8). The major cellular fatty acids were C10 : 0, C10 : 0 3OH, C16 : 0, C17 : 0, C17 : 0 cyclo, C18 : 0, C18 : 3 ω6c (6,9,12), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC). The DNA G+C content of strain THG-YS3.2.7T was 69.4 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.2.7T were identified as Ramlibacter henchirensis DSM 14656T (97.92 %), Ramlibacter tataouinensis DSM 14655T (97.90 %), Ramlibacter solisilvae KACC 17567T (97.04 %). DNA–DNA hybridization values between strain THG-YS3.2.7T and R. henchirensis DSM 14656T, R. tataouinensis DSM 14655T, R. solisilvae KACC 17567T were 32.5±1.5, 43.1±1.1, 42.8±1.1 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-YS3.2.7T represent a novel species of the genus Ramlibacter , for which the name Ramlibacter rhizophilus sp. nov. is proposed. The type strain is THG-YS3.2.7T (=KCTC 52083T=CCTCC AB 2015357T).
-
-
-
Agarilytica rhodophyticola gen. nov., sp. nov., isolated from Gracilaria blodgettii
More LessA novel Gram-stain-negative, rod-shaped, non-spore-forming, aerobic, agarolytic bacterium, designated 017T, was isolated from Gracilaria blodgettii collected at the coast of Lingshui county, Hainan province, China. Optimal growth occurred at 28–33 °C (range 15–40 °C), with 3 % (w/v) NaCl (range 2–4 %) and at pH 8.0 (range pH 6.5–8.5). Cells of strain 017T were motile and formed yellow colonies on marine agar 2216. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 017T shared the highest similarity with Teredinibacter turnerae T7902T (94.4 %). The predominant polar lipids of the novel isolate consisted of phosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and some other unknown lipids. Major cellular fatty acids (>10 %) were C16 : 0, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH), and the sole respiratory lipoquinone was Q-8. The DNA G+C content of strain 017T was 40.2 mol%. Comparative analysis of 16S rRNA gene sequences and phenotypic characterization indicated that strain 017T represents a novel species in a new genus of the family Cellvibrionaceae , order Cellvibrionales , for which the name Agarilytica rhodophyticola gen. nov., sp. nov. is proposed. The type strain of Agarilytica rhodophyticola is 017T (=KCTC 42584T=MCCC 1H00123T).
-
-
-
Pontibacterium granulatum gen. nov., sp. nov., isolated from a tidal flat
More LessA Gram-stain-negative, strictly aerobic, moderately halophilic bacterium, designated A-1T, was isolated from a tidal flat of the Taean coast in South Korea. Cells were motile rods with a single flagellum showing oxidase-negative and catalase-positive activities and contained poly-β-hydroxyalkanoic acid granules. Growth of strain A-1T was observed at 20–40 °C (optimum, 30 °C), pH 6.0–10.5 (optimum, pH 7.0) and in the presence of 1.0–6.0 % (w/v) NaCl (optimum, 2.0 %). Strain A-1T contained C16 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c) as the major fatty acids. The major polar lipids of strain A-1T were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The isoprenoid quinones detected were ubiquinone-7 and ubiquinone-8. The G+C content of the genomic DNA was 51.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A-1T formed a distinct phylogenetic lineage from other genera within the family Oceanospirillaceae . Strain A-1T shared low 16S rRNA gene sequence similarities with other taxa (≤94.9 %). On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that strain A-1T represents a novel genus and species of the family Oceanospirillaceae , for which the name Pontibacterium granulatum gen. nov., sp. nov. is proposed. The type strain is A-1T (=KACC 18119T=JCM 30136T).
-
-
-
Endozoicomonas acroporae sp. nov., isolated from Acropora coral
More LessStrain Acr-14T, isolated from Acropora coral, was characterized by using a polyphasic taxonomy approach. Cells of strain Acr-14T were Gram-stain-negative, aerobic, non-motile, poly-β-hydroxybutyrate-accumulating, rod-shaped and formed creamy white colonies. Optimal growth occurred at 30 °C, pH 7 and in the presence of 2 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Acr-14T belonged to the genus Endozoicomonas and was most closely related to Endozoicomonas atrinae WP70T with sequence similarity of 96.7 %. Strain Acr-14T contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0 as the predominant fatty acids. The predominant isoprenoid quinone was Q-9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content of strain Acr-14T was 49.1 mol%. Differential phenotypic properties, together with the phylogenetic inference, demonstrated that strain Acr-14T should be classified as a novel species of the genus Endozoicomonas , for which the name Endozoicomonas acroporae sp. nov. is presented. The type strain is Acr-14T (=BCRC 80922T=LMG 29482T=KCTC 42901T).
-
-
-
Altererythrobacter deserti sp. nov., isolated from desert soil
More LessA Gram-stain-negative, aerobic, short rod-shaped, non-motile bacterium (THG-S3T), was isolated from desert soil. Growth occurred at 15–35 °C (optimum 28 °C), at pH 5–10 (optimum 7) and at 0–4 % NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-S3T were identified as Altererythrobacter rigui KCTC 42620T (99.0 %), Altererythrobacter dongtanensis KCTC 22672T (97.1 %), Altererythrobacter xinjiangensis CCTCC AB 207166T (96.9 %), Altererythrobacter troitsensis KCTC 12303T (96.9 %). Levels of relatedness among strain THG-S3T and other Altererythrobacter species were lower than 96.0 %. DNA–DNA hybridization values between strain THG-S3T and A. rigui KCTC 42620T, A. dongtanensis KCTC 22672T, A. xinjiangensis CCTCC AB 207166T and A. troitsensis KCTC 12303T were 59.7 % (42.8 %, reciprocal analysis), 45.1 % (36.3 %), 34.7 % (25.1 %) and 15.1 % (12.3 %), respectively. The DNA G+C content of strain THG-S3T was 69 mol%. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and three unidentified lipids The quinone was ubiquinone-10. The major fatty acids were C16 : 0, C17 : 1 ω6c, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c). On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-S3T represents a novel species of the genus Altererythrobacter , for which the name Altererythrobacter deserti sp. nov. is proposed. The type strain is THG-S3T (=KACC 19190T=CGMCC 1.15959T).
-
-
-
Limibaculum halophilum gen. nov., sp. nov., a new member of the family Rhodobacteraceae
More LessA Gram-stain-negative, cream-pigmented, aerobic, non-motile, non-spore-forming and short-rod-shaped bacterial strain, designated CAU 1123T, was isolated from mud from reclaimed land. The strain’s taxonomic position was investigated by using a polyphasic approach. Strain CAU 1123T grew optimally at 37 °C and at pH 7.5 in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CAU 1123T formed a monophyletic lineage within the family Rhodobacteraceae with 93.8 % or lower sequence similarity to representatives of the genera Rubrimonas , Oceanicella , Pleomorphobacterium , Rhodovulum and Albimonas. The major fatty acids were C18 : 1 ω7c and 11-methyl C18 : 1 ω7c and the predominant respiratory quinone was Q-10. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, one unidentified aminolipid and one unidentified lipid. The DNA G+C content was 71.1 mol%. Based on the data from phenotypic, chemotaxonomic and phylogenetic studies, it is proposed that strain CAU 1123T represents a novel genus and novel species of the family Rhodobacteraceae , for which the name Limibaculumhalophilum gen. nov., sp. nov. The type strain is CAU 1123T (=KCTC 52187T, =NBRC 112522T).
-
-
-
Agaribacterium haliotis gen. nov., sp. nov., isolated from abalone faeces
More LessA marine bacterium, named strain feces2T, was isolated from the excreted faeces of an abalone, Haliotis discus hannai. The bacterium was Gram-stain-negative, rod-shaped and had a polar flagellum. It formed a white, small and crater-like colony on an agar plate, and had the capability of degrading agar. Activity of oxidase was positive and that of catalase was negative. Strain feces2T grew at 16 to 40 °C with an optimum of 28–30 °C. The nearly full-length 16S rRNA gene of strain feces2T had the greatest sequence similarity of 92.9 % with Marinibactrumhalimedae Q-192T, followed by of 92.8 % with Teredinibacterturnerae T7902T. Phylogenetic analysis indicated that strain feces2T belonged to the family Cellvibrionaceae , representing an independent clade with an uncultured bacterium clone NEP3-15 (98 % sequence similarity of 16S rRNA gene) derived from the phycosphere of Enteromorphaprolifera. The respiratory quinone was ubiquinone Q-8. The predominant fatty acids consisted of summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c), C16 : 0 and summed feature 3 (C16 : 1 ω6c/C16 : 1ω7c). The polar lipids were identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified amino phospholipid and four unknown lipids. The genomic DNA G+C content was 50.5 mol%. On the basis of polyphasic characterizations, strain feces2T represented a novel species and a novel genus in the family Cellvibrionaceae of the order Cellvibrionales within the Gammaproteobacteria , for which the name Agaribacteriumhaliotis gen. nov., sp. nov. is proposed, with the type strain being feces2T (=MCCC 1A11450T=KCTC 52708T).
-
-
-
Transfer of 13 species of the genus Burkholderia to the genus Caballeronia and reclassification of Burkholderia jirisanensis as Paraburkholderia jirisanensis comb. nov.
More LessA recent study of a group of Burkholderia glathei -like bacteria resulted in the description of 13 novel species of the genus Burkholderia . However, our analysis of phylogenetic positions of these species and their molecular signatures (conserved protein sequence indels) showed that they belong to the genus Caballeronia , and we propose to transfer them to this genus. The reclassified species names are proposed as Caballeronia arationis comb. nov., Caballeronia arvi comb. nov., Caballeronia calidae comb. nov., Caballeronia catudaia comb. nov., Caballeronia concitans comb. nov., Caballeronia fortuita comb. nov., Caballeronia glebae comb. nov., Caballeronia hypogeia comb. nov., Caballeronia pedi comb. nov., Caballeronia peredens comb. nov., Caballeronia ptereochthonis comb. nov., Caballeronia temeraria comb. nov. and Caballeronia turbans comb. nov. It is also proposed to reclassify Burkholderia jirisanensis as Paraburkholderia jirisanensis comb. nov. Based on the results of the polyphasic study, B. jirisanensis had been described as a member of the A-group of the genus Burkholderia and the most closely related to Burkholderia rhizosphaerae , Burkholderia humisilvae and Burkholderia solisilvae currently classified as belonging to the genus Paraburkholderia .
-
-
-
Parasphingopyxis algicola sp. nov., isolated from a marine red alga Asparagopsis taxiformis and emended description of the genus Parasphingopyxis Uchida et al. 2012
More LessAn aerobic, Gram-stain-negative, yellow-pigmented bacterium, designated strain ATAX6-5T, was isolated from a marine red alga, Asparagopsistaxiformis, in South Korea. Cells were non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain ATAX6-5T was observed at 5–35 °C (optimum, 30 °C), at pH 6.0–9.5 (optimum, pH 7.0) and in the presence of 0–6.0 % (w/v) NaCl (optimum, 2 %). Ubiquinone-10 was detected as the sole isoprenoid quinone and C18 : 1ω7c, C16 : 0 and C17 : 1 ω6c were identified as the major cellular fatty acids. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine, an unknown phospholipid and four unknown glycolipids were detected as polar lipids. The G+C content of the genomic DNA was 60.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ATAX6-5T formed a tight phylogenic lineage with Parasphingopyxis lamellibrachiae JAMH 0132T with a 100 % bootstrap value. Strain ATAX6-5T was most closely related to P. lamellibrachiae JAMH 0132T with a 96.9 % 16S rRNA gene sequence similarity. Based on phenotypic, chemotaxonomic and molecular features, strain ATAX6-5T clearly represents a novel species of the genus Parasphingopyxis , for which the name Parasphingopyxis algicola sp. nov. is proposed. The type strain is ATAX6-5T (=KACC 18993T=JCM 31719T). An emended description of the genus Parasphingopyxis is also proposed.
-
-
-
Catenovulum sediminis sp. nov., isolated from coastal sediment
More LessA novel Gram-stain-negative, strictly aerobic, rod-shaped and agar-hydrolysing bacterium, designated D2T, was isolated from a marine sediment sample collected from the coast of Weihai, China (37° 31′ 59″ N 122° 03′ 47″ E). The cells were motile by a lateral flagellum. Growth was observed at 10–42 °C, at pH 6.0–9.0 and with 0.5–8 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain D2T belonged to the genus Catenovulum , appearing closely related to Catenovulum agarivorans YM01T (96.3 % 16S rRNA gene sequence similarity) and Catenovulum maritimum Q1T (93.9 %). The dominant fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and C10 : 0 3-OH. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, two phosphoaminolipids, two unknown lipids and three phospholipids. Ubiquinone 8 (Q-8) was found to be the major respiratory quinone. The DNA G+C content was 40.4 mol%. On the basis of genotypic, phenotypic and phylogenetic evidence, strain D2T is presented as a representative of a novel species of the genus Catenovulum , for which the name Catenovulum sediminis sp. nov. is proposed. The type strain is D2T (=KCTC 42869T=MCCC 1H00129T). An emended description of the genus Catenovulum is also provided.
-
-
-
Sphingomonas frigidaeris sp. nov., isolated from an air conditioning system
Yunho Lee and Che Ok JeonA strictly aerobic Gram-stain-negative bacterium, designated strain KER25-10T, was isolated from a laboratory air conditioning system in South Korea. Cells were yellow-pigmented, non-motile rods showing catalase- and oxidase-positive reactions. The strain grew at pH 4.0–9.0 (optimum, pH 6.0–7.0) and 10–40 °C (optimum, 30 °C) and in the presence of 0–3 % (w/v) NaCl (optimum, 0 %). The G+C content of the genomic DNA was 65.1 mol%. Strain KER25-10T contained ubiquinone-10 (Q-10) as the predominant isoprenoid quinone and C16 : 0, C17 : 1ω6c, summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c) and summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c) as the major fatty acids. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. Only spermidine was detected as the polyamine. Phylogenetic analysis based on 16S rRNA sequences indicated that strain KER25-10T formed a distinct phylogenetic lineage within the genus Sphingomonas of the family Sphingomonadaceae and the strain was most closely related to Sphingomonas kyeonggiense THG-DT81T with a 96.8 % 16S rRNA gene sequence similarity. On the basis of phenotypic, chemotaxonomic and molecular features, strain KER25-10T clearly represents a novel species of the genus Sphingomonas , for which the name Sphingomonas frigidaeris sp. nov. is proposed. The type strain is KER25-10T (=KACC 19285T=JCM 32053T).
-
-
-
Reclassification of Halothiobacillus hydrothermalis and Halothiobacillus halophilus to Guyparkeria gen. nov. in the Thioalkalibacteraceae fam. nov., with emended descriptions of the genus Halothiobacillus and family Halothiobacillaceae
More LessThe genus Halothiobacillus contains four species of obligate autotrophs with validly published names, of which Halothiobacillus halophilus and Halothiobacillus hydrothermalis are very distant from the type species – on the basis of the 16S rRNA gene, they have 90.7 % and 90.9 % identity to that of the type species, Halothiobacillus neapolitanus . As these values fall below the Yarza cut-off for the rank of genus, and these two species also show no clear affiliation to the closely related genus Thioalkalibacter , a polyphasic study was undertaken to determine if they represent a separate genus. Unlike Halothiobacillus spp. sensu stricto, H. halophilus and H. hydrothermalis are halophilic (rather than halotolerant) and moderately alkaliphilic (rather than neutrophilic) and additionally do not produce tetrathionate as a detectable intermediate of thiosulfate metabolism, indicating some significant metabolic differences. On the basis of these data and of functional gene examination, it is proposed that they be circumscribed as a new genus Guyparkeria gen.nov, for which the type species is Guyparkeria halophila gen. nov., comb. nov. Additionally, Thioalkalibacter and Guyparkeria gen. nov. fall distant from the Halothiobacillaceae so the Thioalkalibacteraceae fam. nov. is proposed, for which Thioalkalibacter is the type genus. Emended descriptions of Halothiobacillus , Halothiobacillus neapolitanus and the Halothiobacillaceae are provided.
-
-
-
Rhizobium esperanzae sp. nov., a N 2 -fixing root symbiont of Phaseolus vulgaris from Mexican soils
More LessCommon bean (Phaseolus vulgaris L.) is the most important legume consumed worldwide; its genetic origins lie in the Mesoamerican (main centre) and Andean regions. It is promiscuous in establishing root-nodule symbioses; however, in the centres of origin/domestication, the predominant association is with Rhizobium etli . We have previously identified a new lineage (PEL-3) comprising three strains (CNPSo 661, CNPSo 666 and CNPSo 668T) isolated from root nodules of common bean in Mexico, and that have now been analysed in more detail. Sequences of the 16S rRNA gene positioned the three strains in a large clade including R. etli . Multilocus sequence analysis (MLSA) with four housekeeping genes (recA, glnII, gyrB and rpoA) positioned the three strains in a clade distinct from all other described species, with 100 % bootstrap support, and nucleotide identity (NI) of the four concatenated genes with the closest species R. etli was 95.0 %. Average nucleotide identity (ANI) values of the whole genome of CNPSo 668T and the closest species, R. etli , was 92.9 %. In the analyses of the symbiotic genes nifH and nodC, the strains comprised a cluster with other rhizobial symbionts of P. vulgaris. Other phenotypic and genotypic traits were determined for the new group and our data support the description of the three CNPSo strains as a novel species, for which the name Rhizobium esperanzae is proposed. The type strain is CNPSo 668T (=UMR 1320T=Z87-8T=LMG 30030 T=U 10001T), isolated from a common-bean nodule in Mexico.
-
-
-
Hyphococcus flavus gen. nov., sp. nov., a novel alphaproteobacterium isolated from deep seawater
A Gram-staining-negative, aerobic, non-spore-forming, coccoid to rod shaped bacteria with prosthecate and flagellum, designated as HSF6T, was isolated from deep seawater samples collected from the South China Sea at depth of 2.5 km and subjected to a polyphasic taxonomic investigation. Colonies of strain HSF6T were 1–2 mm in diameter, smooth, circular, convex and yellow. Strain HSF6T was found to grow at 15–37 °C (optimum, 25–35 °C), pH 5.0–9.5 (optimum, pH 7.0–7.5) and with 0–8 % (w/v) NaCl (optimum, 2 %). Chemotaxonomic analysis showed the predominant respiratory quinone of strains HSF6T were ubiquinone-10, and the major fatty acids were C18 : 1ω7c, C16 : 0 and 11-methyl C18 : 1 ω7c. The polar lipids were monoglycosyldiglyceride (MGDG), sulfo-quinovosyl diacylglycerol (SQDG), three unknown glycolipids (GL1–3) and five unknown lipids (L1–5). The DNA G+C content of strain HSF6T was determined to be 51.0 mol% with HPLC. The comparison of 16S rRNA gene sequence similarities show that strain HSF6T was related most closely to genus Parvularcula with similarity ranging from 91.0 to 91.8 %. The phylogenetic trees, using the 16S rRNA gene sequence, reconstructed with neighbour-joining, maximum-parsimony and maximum-likelihood methods showed that strain HSF6T constituted a separated branch in the family ‘ Parvularculaceae ’. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain HSF6T is clearly distinct from validly published genera. On the basis of these features, we propose strain HSF6T (=MCCC 1K03223T=KCTC 52486T) represents a novel species of a novel genus with the name Hyphococcus flavus gen. nov., sp. nov.
-
-
-
Alteromonas pelagimontana sp. nov., a marine exopolysaccharide-producing bacterium isolated from the Southwest Indian Ridge
More LessA novel exopolysaccharide-producing strain, designated as 5.12T, was isolated from a sediment sample from the Southwest Indian Ridge, Indian Ocean. The strain was Gram-stain-negative, motile, strictly aerobic, and oxidase- and catalase-positive. It grew optimally at 35 °C, at pH 6.0 and in the presence of 3.5 % (w/v) NaCl. Its major isoprenoid quinone was ubiquinone-8 (Q-8) and summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and C18 : 1ω7c were the major cellular fatty acids. The DNA G+C content was 46.1 mol%. 16S rRNA gene sequence analysis suggested that strain 5.12T is a member of the genus Alteromonas . Strain 5.12T exhibited close 16S rRNA gene sequence similarity to Alteromonas lipolytica JW12T (96.1 %), Alteromonas hispanica F-32T (95.9 %), Alteromonas confluentis DSSK2-12T (95.9 %), Alteromonas litorea TF-22T (95.6 %) and Alteromonas mediterranea DET (95.5 %). Strain 5.12T contained phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. Owing to significant differences in the 16S rRNA gene sequences, as well as the phenotypic and chemotaxonomic characteristics, the novel isolate described here merits classification as a representative of a novel species of the genus Alteromonas , for which the name Alteromonas pelagimontana sp. nov. is proposed. The type strain of this species is 5.12T (LMG 29661T= MCC 3250T).
-
-
-
Roseomonas aerofrigidensis sp. nov., isolated from an air conditioner
More LessA Gram-stain-negative, strictly aerobic bacterium, designated HC1T, was isolated from an air conditioner in South Korea. Cells were orange, non-motile cocci with oxidase- and catalase-positive activities and did not contain bacteriochlorophyll a. Growth of strain HC1T was observed at 10–45 °C (optimum, 30 °C), pH 4.5–9.5 (optimum, pH 7.0) and 0–3 % (w/v) NaCl (optimum, 0 %). Strain HC1T contained summed feature 8 (comprising C18 : 1ω7c/C18 : 1ω6c), C16 : 0 and cyclo-C19 : 0ω8c as the major fatty acids and ubiquinone-10 as the sole isoprenoid quinone. Phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unknown aminolipid were detected as the major polar lipids. The major carotenoid was hydroxyspirilloxanthin. The G+C content of the genomic DNA was 70.1 mol%. Phylogenetic analysis, based on 16S rRNA gene sequences, showed that strain HC1T formed a phylogenetic lineage within the genus Roseomonas . Strain HC1T was most closely related to the type strains of Roseomonas oryzae , Roseomonas rubra , Roseomonas aestuarii and Roseomonas rhizosphaerae with 98.1, 97.9, 97.6 and 96.8 % 16S rRNA gene sequence similarities, respectively, but the DNA–DNA relatedness values between strain HC1T and closely related type strains were less than 70 %. Based on phenotypic, chemotaxonomic and molecular properties, strain HC1T represents a novel species of the genus Roseomonas , for which the name Roseomonas aerofrigidensis sp. nov. is proposed. The type strain is HC1T (=KACC 19097T=JCM 31878T).
-
-
-
Hydrogenophaga crassostreae sp. nov., isolated from a Pacific oyster
More LessA Gram-negative, motile, rod-shaped, and aerobic bacterial strain, designated LPB0072T, was isolated from a Pacific oyster (Crassostrea gigas). Autotrophic growth with hydrogen gas was not observed. Cells oxidized thiosulfate to sulfate and reduced nitrate to nitrite. The complete genome sequence of strain LPB0072T (CP017476) is 4.94 Mb in length and contains 4459 protein-coding genes, with a G+C content of 61.3 mol%. Analysis of the 16S rRNA gene sequence indicated that strain LPB0072T belongs to the genus Hydrogenophaga , with greatest sequence similarity to the type strain of Hydrogenophaga taeniospiralis (97.5 %). The isoprenoid quinone (Q-8) and the major cellular fatty acids (C16 : 1ω7c and/or C16 : 1ω6c, C16 : 0 and C17 : 1ω6c) identified were concordant with the chemotaxonomic properties of the genus Hydrogenophaga . The average nucleotide identities with closely related species were below the suggested boundary for species delineation, indicating that the isolate is a novel species. Numerous physiological and biochemical features also distinguished the isolate from other known Hydrogenophaga species. Based on the polyphasic data presented in this study, strain LPB0072T should be classified as a novel species in the genus Hydrogenophaga , and the name Hydrogenophaga crassostreae sp. nov. is proposed. The type strain is LPB0072T (=KACC 18705T=JCM 31188T).
-
-
-
Sphingomonas jeddahensis sp. nov., isolated from Saudi Arabian desert soil
A novel Sphingomonas strain was isolated from a sample of desert soil collected near Jeddah in Saudi Arabia. A polyphasic approach was performed to characterize this strain, initially designated as G39T. Cells of strain G39T are motile, Gram-negative, catalase- and oxidase-positive. The strain is able to grow aerobically at 20–35 °C, pH 6.5–8 and tolerates up to 4 % (w/v) NaCl. Based on 16S rRNA gene sequence similarity, the closest relative type strains of G39T are Sphingomonas mucosissima DSM 17494T (98.6 %), S. dokdonensis DSM 21029T (98.4 %) and S. hankookensis DSM 23329T (97.4 %). Furthermore, the average nucleotide identities between the draft genome sequence of strain G39T and the genome sequences of all other available and related Sphingomonas species are significantly below the threshold of 94 %. The G+C content of the draft genome (3.12 Mbp) is 65.84 %. The prevalent (>5 %) cellular fatty acids of G39T were C18 : 1 ω7c, C16 : 1 ω7c and/or C16 : 1 ω6c, C14 : 0 2-OH and C16 : 0. The only detectable respiratory quinone was ubiquinone-10 and the polar lipids profile is composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, as well as unidentified lipids, phospholipids and glycolipids. The results of the conducted polyphasic approach confirmed that this isolate represents a novel species of the genus Sphingomonas , for which the name Sphingomonas jeddahensis sp. nov. is proposed. The type strain of this species is G39T (=DSM 103790T=LMG 29955T).
-
-
-
Sphingomonas antarctica sp. nov., isolated from Antarctic tundra soil
Strain 200T, isolated from a soil sample taken from Antarctic tundra soil around Zhongshan Station, was found to be a Gram-stain-negative, yellow-pigmented, catalase-positive, oxidase-negative, non-motile, non-spore-forming, rod-shaped and aerobic bacterium. Strain 200T grew optimally at pH 7.0 and in the absence of NaCl on R2A. Its optimum growth temperature was 20 °C. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 200T belonged to the genus Sphingomonas . Strain 200T showed the highest sequence similarities to Sphingomonas kyeonggiense THG-DT81T (95.1 %) and Sphingomonas molluscorum KMM 3882T (95.1 %). Chemotaxonomic analysis showed that strain 200T had characteristics typical of members of the genus Sphingomonas . Ubiquinone 10 was the predominant respiratory quinone and sym-homospermidine was the polyamine. The major polar lipids were sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. The G+C content of the genomic DNA was determined to be 60.9 mol%. Strain 200T contained C16 : 0 (31.6 %), summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c, 22.7 %), summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c, 11.2 %), C18 : 0 (7.8 %) and C14 : 0 2OH (6.7 %) as the major cellular fatty acids. On the basis of phylogenetic analysis, and physiological and biochemical characterization, strain 200T should be classified as representing a novel species of the genus Sphingomonas , for which the name Sphingomonas antarctica sp. nov. is proposed. The type strain is 200T (=CCTCC AB 2016064T=KCTC 52488T).
-
-
-
Sphingomonas carri sp. nov., isolated from a car air-conditioning system
A Gram-stain-negative, yellow-pigmented bacterial strain, designated PR0302T, was isolated from a car evaporator core collected in Korea. The cells were strictly aerobic, non-spore-forming and rod-shaped. The strain grew at 15–37 °C (optimum, 25 °C), at pH 6.0–8.0 (optimum, 7.0) and in the presence of 0–1 % (w/v) NaCl. Phylogenetically, the strain was closely related to members of the genus Sphingomonas (97.04–91.22 % 16S rRNA gene sequence similarities) and showed the highest sequence similarity of 97.04 % to Sphingomonas kyeonggiensis THG-DT81T. It contained C16 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C14 : 0 2-OH as the predominant fatty acids and Q-10 as the major ubiquinone. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid. The major polyamine was sym-homospermidine. The serine palmitoyl transferase gene (spt) was detected and sphingolipid synthesis was confirmed. The mean DNA G+C content of the strain was 67.8±0.5 mol%. DNA–DNA relatedness between strain PR0302T and closely related type strains of Sphingomonas species was less than 30 %. The low levels of DNA–DNA relatedness identified strain PR0302T as a member of a novel species in the genus Sphingomonas . Based on phenotypic, genotypic and chemotaxonomic data, strain PR0302T represents a novel species in the genus Sphingomonas , for which the name Sphingomonas carri sp. nov. is proposed. The type strain is PR0302T (=KACC 18487T=NBRC 111532T).
-
Volumes and issues
-
Volume 74 (2024)
-
Volume 73 (2023)
-
Volume 72 (2022 - 2023)
-
Volume 71 (2020 - 2021)
-
Volume 70 (2020)
-
Volume 69 (2019)
-
Volume 68 (2018)
-
Volume 67 (2017)
-
Volume 66 (2016)
-
Volume 65 (2015)
-
Volume 64 (2014)
-
Volume 63 (2013)
-
Volume 62 (2012)
-
Volume 61 (2011)
-
Volume 60 (2010)
-
Volume 59 (2009)
-
Volume 58 (2008)
-
Volume 57 (2007)
-
Volume 56 (2006)
-
Volume 55 (2005)
-
Volume 54 (2004)
-
Volume 53 (2003)
-
Volume 52 (2002)
-
Volume 51 (2001)
-
Volume 50 (2000)
-
Volume 49 (1999)
-
Volume 48 (1998)
-
Volume 47 (1997)
-
Volume 46 (1996)
-
Volume 45 (1995)
-
Volume 44 (1994)
-
Volume 43 (1993)
-
Volume 42 (1992)
-
Volume 41 (1991)
-
Volume 40 (1990)
-
Volume 39 (1989)
-
Volume 38 (1988)
-
Volume 37 (1987)
-
Volume 36 (1986)
-
Volume 35 (1985)
-
Volume 34 (1984)
-
Volume 33 (1983)
-
Volume 32 (1982)
-
Volume 31 (1981)
-
Volume 30 (1980)
-
Volume 29 (1979)
-
Volume 28 (1978)
-
Volume 27 (1977)
-
Volume 26 (1976)
-
Volume 25 (1975)
-
Volume 24 (1974)
-
Volume 23 (1973)
-
Volume 22 (1972)
-
Volume 21 (1971)
-
Volume 20 (1970)
-
Volume 19 (1969)
-
Volume 18 (1968)
-
Volume 17 (1967)
-
Volume 16 (1966)
-
Volume 15 (1965)
-
Volume 14 (1964)
-
Volume 13 (1963)
-
Volume 12 (1962)
-
Volume 11 (1961)
-
Volume 10 (1960)
-
Volume 9 (1959)
-
Volume 8 (1958)
-
Volume 7 (1957)
-
Volume 6 (1956)
-
Volume 5 (1955)
-
Volume 4 (1954)
-
Volume 3 (1953)
-
Volume 2 (1952)
-
Volume 1 (1951)