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Volume 65,
Issue Pt_7,
2015
Volume 65, Issue Pt_7, 2015
- NEW TAXA
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- Proteobacteria
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Youhaiella tibetensis gen. nov., sp. nov., isolated from subsurface sediment
More LessA Gram-reaction-negative bacterial strain, designated fig4T, was isolated from a subsurface sediment core of Qiangtang Basin permafrost in China. Cells were catalase- and oxidase-positive and rods. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain fig4T was a member of the family Hyphomicrobiaceae and was most closely related to members of the genera Pelagibacterium, Vasilyevaea and Devosia with 93.8–96.2 % sequence similarities. The major cellular fatty acids were C16 : 0, C18 : 0, 11-methyl C18 : 1ω7c, C19 : 0 cyclo ω8c and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The major respiratory quinone was Q-10 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The DNA G+C content was 60.7 mol%. Based on the phenotypic, phylogenetic and genotypic data, strain fig4T is considered to represent a novel species of a new genus in the family Hyphomicrobiaceae, for which the name Youhaiella tibetensis gen. nov., sp. nov. is proposed. The type strain is fig4T ( = CGMCC 1.12719T = JCM 19854T).
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Marinobacter nitratireducens sp. nov., a halophilic and lipolytic bacterium isolated from coastal surface sea water
More LessA novel Gram-stain-negative, rod-shaped, motile bacterium, designated strain AK21T, was isolated from coastal surface sea water at Visakhapatnam, India. The strain was positive for oxidase, catalase, lipase, l-proline arylamidase and tyrosine arylamidase activities. The predominant fatty acids were C12:0, C12:0 3-OH, C16:0, C16:1ω9c, C18:1ω9c and summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, two unidentified phospholipids and one unidentified lipid. Q-10 was the predominant respiratory quinone. The DNA G+C content of the strain was 54.6 mol%. 16S rRNA gene sequence analysis indicated that strain AK21T was a member of the genus Marinobacter and was closely related to Marinobacter xestospongiae, with pairwise sequence similarity of 97.2 % to the type strain, with similarity to other members of the genus of 94.0–96.8 %. The mean DNA–DNA relatedness of strain AK21T with M. xestospongiae JCM 17469T was 34.5 %, and relatedness with Marinobacter mobilis JCM 15154T was 40.5 %. Phylogenetic analysis showed that strain AK21T clustered with the type strains of M. xestospongiae and M. mobilis at distances of 2.9 and 2.8 % (97.1 and 97.2 % similarity), respectively. Based on the phenotypic characteristics and on phylogenetic inference, it appears that strain AK21T represents a novel species of the genus Marinobacter, for which the name Marinobacter nitratireducens sp. nov. is proposed. The type strain of Marinobacter nitratireducens is AK21T ( = MTCC 11704T = JCM 18428T).
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Pseudomonas endophytica sp. nov., isolated from stem tissue of Solanum tuberosum L. in Spain
A bacterial strain named BSTT44T was isolated in the course of a study of endophytic bacteria occurring in stems and roots of potato growing in a soil from Salamanca, Spain. The 16S rRNA gene sequence had 99.7 % identity with respect to that of its closest relative, Pseudomonas psychrophila E-3T, and the next most closely related type strains were those of Pseudomonas fragi, with 99.6 % similarity, Pseudomonas deceptionensis, with 99.2 % similarity, and Pseudomonas lundensis, with 99.0 % similarity; these results indicate that BSTT44T should be classified within the genus Pseudomonas. Analysis of the housekeeping genes rpoB, rpoD and gyrB confirmed its phylogenetic affiliation and showed identities lower than 92 % in all cases with respect to the above-mentioned closest relatives. Cells of the strain bore one polar–subpolar flagellum. The respiratory quinone was Q-9.The major fatty acids were C16:0, C18:1ω7c and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The strain was oxidase-, catalase- and urease-positive and the arginine dihydrolase system was present, but tests for nitrate reduction, β-galactosidase production and aesculin hydrolysis were negative. It could grow at 35 °C and at pH 5–9.The DNA G+C content was 60.2 mol%. DNA–DNA hybridization results showed less than 48 % relatedness with respect to the type strains of the four most closely related species. Therefore, the combined results of genotypic, phenotypic and chemotaxonomic analyses support the classification of strain BSTT44 into a novel species of the genus Pseudomonas, for which the name Pseudomonas endophytica sp. nov. is proposed. The type strain is BSTT44T ( = LMG 28456T = CECT 8691T).
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Massilia eurypsychrophila sp. nov. a facultatively psychrophilic bacteria isolated from ice core
Strain B528-3T, a Gram-stain-negative, rod-shaped, aerobic, facultatively psychrophilic bacterium with polar flagella, was isolated from an ice core drilled from Muztagh Glacier, Xinjiang, China. The novel isolate was classified into the genus Massilia. The 16S rRNA gene sequence of the novel isolate shares a pairwise similarity of less than 97 % with those of all the type strains of the genus Massilia. The major fatty acids of strain B528-3T were summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH) (57.31 %), C16:0 (11.46 %) and C18:1ω7c (14.72 %). The predominant isoprenoid quinone was Q-8. The DNA G+C content was 62.2 mol% (T m). The major polar lipids of this bacterium were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. From the genotypic and phenotypic data, it is evident that strain B528-3T represents a novel species of the genus Massilia, for which the name Massilia eurypsychrophila sp. nov. is proposed. The type strain is B528-3T ( = JCM 30074T = CGMCC 1.12828T).
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Alcanivorax gelatiniphagus sp. nov., a marine bacterium isolated from tidal flat sediments enriched with crude oil
More LessA Gram-reaction-negative, rod-shaped marine bacterium, designated MEBiC08158T, was isolated from sediments collected from Taean County, Korea, near the Hebei Spirit tanker oil spill accident. 16S rRNA gene sequence analysis revealed that strain MEBiC08158T was closely related to Alcanivorax marinus R8-12T (99.5 % similarity) but was distinguishable from other members of the genus Alcanivorax (93.7–97.1 %). The DNA–DNA hybridization value between strain MEBiC08158T and A. marinus R8-12T was 58.4 %. Growth of strain MEBiC08158T was observed at 15–43 °C (optimum 37–40 °C), at pH 6.0–9.5 (optimum pH 7.0–8.0) and with 0.5–16 % NaCl (optimum 1.5–3.0 %). The dominant fatty acids were C16 : 0, C19 : 0 cyclo ω8c, C12 : 0, C18 : 1ω7c, C12 : 0 3-OH and summed feature 3 (comprising C15 : 0 2-OH and/or C16 : 1ω7c). Several phenotypic characteristics differentiate strain MEBiC08158T from phylogenetically close members of the genus Alcanivorax. Therefore, strain MEBiC08158T should be classified as representing a novel species of the genus Alcanivorax, for which the name Alcanivorax gelatiniphagus sp. nov. is proposed. The type strain is MEBiC08158T ( = KCCM 42990T = JCM 18425T).
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Functional and genomic diversity of methylotrophic Rhodocyclaceae: description of Methyloversatilis discipulorum sp. nov.
Three strains of methylotrophic Rhodocyclaceae (FAM1T, RZ18-153 and RZ94) isolated from Lake Washington sediment samples were characterized. Based on phylogenetic analysis of 16S rRNA gene sequences the strains should be assigned to the genus Methyloversatilis. Similarly to other members of the family, the strains show broad metabolic capabilities and are able to utilize a number of organic acids, alcohols and aromatic compounds in addition to methanol and methylamine. The main fatty acids were 16:1ω7c (49–59 %) and 16:0 (32–29 %). Genomes of all isolates were sequenced, assembled and annotated in collaboration with the DOE Joint Genome Institute (JGI). Genome comparison revealed that the strains FAM1T, RZ18-153 and RZ94 are closely related to each other and almost equally distant from two previously described species of the genus Methyloversatilis, Methyloversatilis universalis and Methyloversatilis thermotolerans. Like other methylotrophic species of the genus Methyloversatilis, all three strains possess one-subunit PQQ-dependent ethanol/methanol dehydrogenase (Mdh-2), the N-methylglutamate pathway and the serine cycle (isocitrate lyase/malate synthase, Icl/ms+ variant). Like M. universalis, strains FAM1T, RZ18-153 and RZ94 have a quinohemoprotein amine dehydrogenase, a tungsten-containing formaldehyde ferredoxin oxidoreductase, phenol hydroxylase, and the complete Calvin cycle. Similarly to M. thermotolerans, the three strains possess two-subunit methanol dehydrogenase (MxaFI), monoamine oxidase (MAO) and nitrogenase. Based on the phenotypic and genomic data, the strains FAM1T, RZ18-153 and RZ94 represent a novel species of the genus Methyloversatilis, for which the name Methyloversatilis discipulorum sp. nov. is proposed. The type strain is FAM1T ( = JCM 30542T = VKM = B-2888T).
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Mameliella atlantica sp. nov., a marine bacterium of the Roseobacter clade isolated from deep-sea sediment of the South Atlantic Ocean
More LessA taxonomic study was carried out on strain L6M1-5T, which was isolated from deep-sea sediment collected from the South Atlantic Ocean. The isolate was Gram-reaction-negative, oxidase-negative and catalase-weakly positive. Growth was observed in the presence of 0.5–15 % (w/v) NaCl (optimum 3–5 %), at 10–41 °C (optimum 28–30 °C), and pH 5.0–10.5 (optimum pH 7.0). The principal fatty acids were summed feature8 (C18 : 1ω7c/ω6c) (84.2 %), C18 : 0 (6.3 %), C12 : 1 3-OH (3.2 %) and C16 : 0 (2.7 %). The polar lipid profile comprised phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, two unknown phospholipids and one unknown lipid. Ubiquinone-10 was the major quinone. The G+C content of the genomic DNA was 66.0 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain L6M1-5T belonged to the genus Mameliella and shared 95.8 % sequence similarity with Mameliella alba JLT354-WT. The combined genotypic and phenotypic data show that strain L6M1-5T represents a novel species of the genus Mameliella, for which the name Mameliella. atlantica sp. nov. is proposed. The type strain is L6M1-5T ( = MCCC 1A07531T = JCM 30230T).
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Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources
Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9 %, respectively) and towards established Bcc species (4.0 and 3.9 %, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156T ( = CCUG 65686T) and Burkholderia territorii sp. nov. with type strain LMG 28158T ( = CCUG 65687T) are proposed for Bcc B and Bcc L bacteria, respectively.
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Kangiella profundi sp. nov., isolated from deep-sea sediment
More LessA taxonomic study employing a polyphasic approach was carried out on strain FT102T, which was isolated from a deep-sea sediment sample collected in the south-west Indian Ocean at a depth of 2784 m. The strain was Gram-stain-negative, non-motile, rod-shaped and non-spore-forming. It grew optimally at 37–42 °C, pH 6.5–8.5 and in the presence of 1–4 % (w/v) NaCl. Phylogenetic analysis of 16S rRNA gene sequences confirmed the separation of the novel strain from recognized members of the genus Kangiella that are available in public databases. Strain FT102T exhibited 95.5–98.6 % 16S rRNA gene sequence similarity to the type strains of the eight recognized species of the genus Kangiella. The chemotaxonomically characteristic fatty acid iso-C15:0 and ubiquinone Q-8 were also detected. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The DNA G+C content of strain FT102T was 45.0 mol%. The mean DNA–DNA relatedness values between strain FT102T and the type strains of Kangiella aquimarina and Kangiella koreensis were 47.3 % and 13.7 %, respectively. The combined results of phylogenetic, physiological and chemotaxonomic studies indicated that strain FT102T was affiliated with the genus Kangiella but differed from the recognized species of the genus Kangiella. Therefore, strain FT102T represents a novel species of the genus Kangiella, for which the name Kangiella profundi sp. nov. is proposed. The type strain is FT102T ( = CGMCC 1.12959T = KCTC 42297T = JCM 30232T)
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Roseovarius antarcticus sp. nov., isolated from a decayed whale bone
A pale yellow, ovoid- to rod-shaped and budding bacterium, designated strain M-S13-148T, was isolated from a decayed bone of whale from the eastern coast of King George Island, South Shetlands, Antarctica. Strain M-S13-148T exhibited motility, aerobic growth and was Gram-stain-negative. Strain M-S13-148T was positive for catalase and oxidase. Growth was observed at pH 6.0–9.0, at 4–42 °C and with 0–14 % (w/v) NaCl. The novel strain contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and an unknown phospholipid as the major polar lipids. The dominant cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), (58.8 %) and C16 : 0 (11.7 %). The respiratory quinone was Q-10 and the DNA G+C content was 60.9 mol%. Neighbour-joining, maximum-likelihood and minimum-evolution phylogenetic trees, based on 16S rRNA gene sequences, indicated that strain M-S13-148T belonged to the genus Roseovarius and was most closely related to Roseovarius nanhaiticus CCTCC AB 208317T (93.72 % 16S rRNA gene sequence similarity). The 16S rRNA gene sequence similarity with respect to members of the genus Roseovarius ranged from 91.81 to 93.94 %. On the basis of phenotypic, molecular and chemotaxonomic characteristics, strain M-S13-148 is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius antarcticus sp. nov., is proposed. The type strain is M-S13-148T ( = CCTCC AB2014072T = LMG 28420T).
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- Bacteroidetes
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Mucilaginibacter vulcanisilvae sp. nov., isolated from a volcanic forest
More LessA Gram-stain-negative, strictly aerobic, pale-pink-pigmented bacterium, designated strain G27T, was isolated from a volcanic forest of Jeju Island, South Korea. Cells were catalase- and oxidase-positive, non-motile rods producing exopolysaccharide and flexirubin-type and pale pink pigments. Growth of strain G27T was observed at 4–30 °C (optimum, 25 °C) and pH 5.0–8.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain G27T formed a phyletic lineage within the genus Mucilaginibacter of the family Sphingobacteriaceae. Strain G27T was related most closely to Mucilaginibacter gossypii Gh-67T at 96.5 % 16S rRNA gene sequence similarity. Strain G27T contained menaquinone 7 (MK-7) as the sole isoprenoid quinone and summed feature 3 (comprising C16: 1ω7c/C16: 1ω6c), iso-C15: 0, C16: 0, C16: 1ω5c and C16: 1ω5c as the major cellular fatty acids. Phosphatidylethanolamine was identified as the major polar lipid. The G+C content of the genomic DNA was 46.5 mol%. On the basis of phenotypic, chemotaxonomic and molecular properties, strain G27T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter vulcanisilvae sp. nov. is proposed. The type strain is G27T ( = KACC 18231T = JCM 30363T).
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Maribacter spongiicola sp. nov. and Maribacter vaceletii sp. nov., isolated from marine sponges, and emended description of the genus Maribacter
More LessTwo Gram-stain-negative, rod-shaped, orange, catalase- and oxidase-positive, non-motile bacteria, designated W13M1AT and W15M10T, were isolated from the marine sponges Suberites carnosus and Leucosolenia sp., respectively, which were sampled from Lough Hyne, Co. Cork, Ireland. Analysis of the 16S rRNA gene sequences of these isolates revealed that they are members of the genus Maribacter, in the family Flavobacteriaceae of the phylum Bacteroidetes. The type strain most closely related to strain W13M1AT is Maribacter forsetii DSM 18668T with a gene sequence similarity of 96.5 %. The closest related type strain to strain W15M10T is Maribacter orientalis DSM 16471T with a gene sequence similarity of 98.3 %. Phylogenetic inference and phenotypic data combined indicate that the isolates represent two novel species of the genus Maribacter, for which the names Maribacter spongiicola sp. nov. with type strain W15M10T ( = NCIMB 14725T = DSM 25233T) and Maribacter vaceletii sp. nov. with type strain W13M1AT ( = NCIMB 14724T = DSM 25230T), are proposed.
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Cecembia rubra sp. nov., a thermophilic bacterium isolated from a hot spring sediment
A Gram-staining negative, rod-shaped bacterium, designated strain YIM 78110T, was isolated from a sediment sample collected from Hehua hot spring in Tengchong, Yunnan province, south-west China. The taxonomic status of strain YIM 78110T was confirmed by a polyphasic approach. 16S rRNA gene sequence analysis indicated that strain YIM 78110T belongs to the genus Cecembia, displaying 96.8 % and 94.7 % sequence similarity with the two most closely related type strains, Cecembia calidifontis RQ-33T and Cecembia lonarensis LW9T, respectively. The low value of DNA–DNA hybridization (52.3 ± 2.3 %) between strain YIM 78110T and its closest neighbour, Cecembia calidifontis RQ-33T, indicated that this new isolate represented a different genomic species in the genus Cecembia. The temperature for growth ranged from 30 to 50 °C. The pH for growth ranged from pH 4.0 to 10.0, with NaCl tolerance of 0.5–6.0 % (w/v). The predominant menaquinone of strain YIM 78110T was MK-7 and the major polar lipid was phosphatidylethanolamine. The major fatty acids were iso-C15:0 and C15:0. The DNA G+C content was 47.1 mol%. On the basis of physiological, biochemical and phylogenetic analyses, it is proposed that strain YIM 78110T represents a novel species of the genus Cecembia, for which the name Cecembia rubra sp. nov. is proposed. The type strain is YIM 78110T ( = CCTCC AB2013287T = DSM 28057T).
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Chryseobacterium arachidiradicis sp. nov., isolated from the geocarposphere (soil around the peanut) of very immature peanuts (Arachis hypogaea)
More LessA yellow-pigmented bacterial strain, 91A-612T, isolated from the geocarposphere (soil around the peanut) of very immature peanuts (Arachis hypogaea) in Alabama, USA, was studied for its taxonomic position. Cells of the isolate were rod-shaped and stained Gram-negative. A comparison of the 16S rRNA gene sequence with the sequences of the type strains of the most closely related species showed that the strain belongs to the genus Chryseobacterium, showing the highest sequence similarities to the type strains of Chryseobacterium molle (98.4 %), C. pallidum (98.3 %) and C. hominis (97.8 %). The 16S rRNA gene sequence similarities to the type strains of all other species of the genus Chryseobacterium were below 97.0 %. The fatty acid profile of strain 91A-612T consisted of the major fatty acids iso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH/C16 : 1ω7c) and iso-C17 : 0 3-OH. Major compounds in the polar lipid profile were phosphatidylethanolamine and several unidentified lipids, including two lipids that did not contain a sugar moiety, an amino group or a phosphate group (L3, L8), and an aminolipid (AL1). The quinone system was composed mainly of MK-6. The polyamine pattern contained sym-homospermidine as the major compound and moderate amounts of spermidine and spermine. DNA–DNA hybridizations between strain 91A-612T and the type strains of C. molle, C. pallidum and C. hominis resulted in relatedness values well below 70 %. These data and the differentiating biochemical and chemotaxonomic properties showed that isolate 91A-612T represents a novel species of the genus Chryseobacterium, for which we propose the name Chryseobacterium arachidiradicis sp. nov. (type strain 91A-612T = LMG 27814T = CCM 8490T = CIP 110647T).
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Elizabethkingia endophytica sp. nov., isolated from Zea mays and emended description of Elizabethkingia anophelis Kämpfer et al. 2011
More LessA slightly yellow bacterial strain (JM-87T), isolated from the stem of healthy 10 day-old sweet corn (Zea mays), was studied for its taxonomic allocation. The isolate revealed Gram-stain-negative, rod-shaped cells. A comparison of the 16S rRNA gene sequence of the isolate showed 99.1, 97.8, and 97.4 % similarity to the 16S rRNA gene sequences of the type strains of Elizabethkingia anophelis, Elizabethkingia meningoseptica and Elizabethkingia miricola, respectively. The fatty acid profile of strain JM-87T consisted mainly of the major fatty acids C15:0 iso, C17:0 iso 3-OH, and C15:0 iso 2-OH/C16:1ω7c/t. The quinone system of strain JM-87T contained, exclusively, menaquinone MK-6. The major polyamine was sym-homospermidine. The polar lipid profile consisted of the major lipid phosphatidylethanolamine plus several unidentified aminolipids and other unidentified lipids. DNA–DNA hybridization experiments with E. meningoseptica CCUG 214T ( = ATCC 13253T), E. miricola KCTC 12492T ( = GTC 862T) and E. anophelis R26T resulted in relatedness values of 17 % (reciprocal 16 %), 30 % (reciprocal 19 %), and 51 % (reciprocal 54 %), respectively. These DNA–DNA hybridization results, in addition to some differentiating biochemical properties, clearly indicate that strain JM-87T is a representative of a novel species, for which the name Elizabethkingia endophytica sp. nov. is proposed. The type strain is JM-87T ( = CIP 110885T = LMG 28604T = CCM 8570T).
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Phaeocystidibacter marisrubri sp. nov., a member of the family Cryomorphaceae isolated from Red Sea sediment
More LessStrain G18T, a Gram-stain-negative, aerobic, rod-shaped, motile, non-fermentative, yellow-pigmented bacterium, was isolated from Red Sea sediment. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain G18T was most closely related to Phaeocystidibacter luteus PG2S01T with 95.3 % similarity. Growth of strain G18T occurred at 10–42 °C (optimum 28–37 °C), pH 5.0–9.0 (optimum pH 6.0–8.0) and in the presence of 0.5–10 % NaCl (optimum 2–5 %). The major fatty acids of strain G18T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 1 G and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, unidentified aminolipids, phospholipids and other lipids. The predominant quinone was menaquinone 6 (MK-6). The G+C content of the genomic DNA from strain G18T was 39.0 mol%. Based on the phylogenetic and phenotypic properties, strain G18T represents a novel species of the genus Phaeocystidibacter, for which the name Phaeocystidibacter marisrubri sp. nov. is proposed. The type strain is G18T ( = CGMCC 1.14954T = JCM 30614T).
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Flaviaesturariibacter amylovorans gen. nov., sp. nov., a starch-hydrolysing bacterium, isolated from estuarine water
More LessA novel bacterial strain, designated GCR0105T, was isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment, located in JEOLlabuk-do, South Korea. Cells of strain GCR0105T were Gram-stain-negative, non-motile and rod-shaped. Colonies of strain GCR0105T were pale yellow-pigmented on R2A agar and nutrient agar media, and were able to grow at 15–30 °C (optimum 25 °C) and pH 6.5–8.5 (optimum pH 7.5). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GCR0105T was related most closely to Flavisolibacter ginsengisoli Gsoil 643T (93.14 % similarity). The polar lipid profile of strain GCR0105T comprised phosphatidylethanolamine, two unknown aminolipids, an unknown aminophospholipid and four unknown lipids. The DNA G+C content of strain GCR0105T was 42.9 mol% and the respiratory quinone was MK-7.On the basis of phenotypic, chemotaxonomic and phylogenetic properties, strain GCR0105T represents a novel species in a new genus within the family Chitinophagaceae, for which the name Flaviaesturariibacter amylovorans gen. nov., sp. nov. is proposed. The type strain of Flaviaesturariibacter amylovorans is GCR0105T ( = KACC 16454T = JCM 17919T).
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Dokdonia pacifica sp. nov., isolated from seawater
More LessA Gram-stain-negative, aerobic, non-flagellated, non-gliding, oxidase- and catalase-positive, rod-shaped, yellow-pigmented bacterium, designated strain SW230T, was isolated from a surface seawater sample collected from the South Pacific Gyre. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SW230T shared highest similarity with members of the genus Dokdonia (95.0–94.5 %), exhibiting 95.0 % sequence similarity to Dokdonia genika NBRC 100811T. Optimal growth occurred in the presence of 2–3 % (w/v) NaCl, at pH 8.0 and at 28 °C. The DNA G+C content of strain SW230T was 36 mol%. The major fatty acids (>10 % of the total) were iso-C15:1 G, iso-C15:0, iso-C17:0 3-OH, and C16:1 ω7c and/or C16:1ω6c. The major respiratory quinone was menaquinone-6. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. On the basis of data from the present polyphasic study, strain SW230T is considered to represent a novel species of the genus Dokdonia, for which the name Dokdonia pacifica sp. nov. is proposed. The type strain is SW230T ( = CGMCC 1.12184T = JCM 18216T).
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Algoriphagus trabzonensis sp. nov., isolated from freshwater, and emended description of Algoriphagus alkaliphilus
More LessA Gram-staining-negative, non-motile, catalase- and oxidase-positive strain, designated MS7T, was isolated from freshwater of a river near Trabzon, Turkey. Its taxonomy was investigated using a polyphasic approach. The strain grew optimally at 28 °C and pH 7.5 and in the presence of 2.0 % NaCl. 16S rRNA gene sequence analysis revealed that the strain belonged to the genus Algoriphagus; strain MS7T showed highest sequence similarity to the type strains of Algoriphagus alkaliphilus (97.3 %), Algoriphagus terrigena (96.8 %), Algoriphagus jejuensis (96.2 %), Algoriphagus boritolerans (96.1 %) and Algoriphagus aquatilis (95.8 %). The major fatty acids of strain MS7T were iso-C15 : 0 (30.14 %) and summed future 9 (10-methyl C16 : 0 and/or iso-C17 : 1 ω9c 18.75 %). Polar lipid analysis revealed phosphatidylethanolamine, a variety of unidentified lipids, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified aminolipid. The major isoprenoid quinone was MK-7.The DNA G+C content of MS7T was 41.6 mol%, a value consistent with that of members of the genus Algoriphagus. The level of DNA–DNA relatedness between strain MS7T and A. alkaliphilus LMG 22694T was 41 %, which is clearly below the 70 % threshold accepted for species delineation. Thus, our results support the placement of strain MS7T within a separate and previously unrecognized species. On the basis of these data, the strain is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus trabzonensis sp. nov. is proposed. The type strain is MS7T ( = NCCB 100372T = LMG 26290T). An emended description of A. alkaliphilus is also provided.
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Mesonia hippocampi sp. nov., isolated from the brood pouch of a diseased Barbour's Seahorse (Hippocampus barbouri)
An orange-pigmented, Gram-staining-negative, rod-shaped bacterium, designated 96_Hippo_TS_3/13T was isolated from the brood pouch of a diseased seahorse male of the species Hippocampus barbouri from the animal facility of the University of Giessen, Germany. Phylogenetic analyses based on the nearly full-length 16S rRNA gene sequence placed strain 96_Hippo_TS_3/13T into the monophyletic cluster of the genus Mesonia within the family Flavobacteriaceae. However, the strain shared only 92.2–93.8 % sequence similarity to type strains of species of the genus Mesonia, with highest sequence similarity to the type strain of Mesonia aquimarina. Cellular fatty acid analysis showed a Mesonia-typical fatty acid profile including several branched and hydroxyl fatty acids with highest amounts of iso-C15 : 0 (40.9 %) followed by iso-C17 : 0 3-OH (14.8 %). In the polyamine pattern, sym-homospermidine was predominant. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The quinone system contained exclusively menaquinone MK-6. The only identified compound in the polar lipid profile was phosphatidylethanolamine present in major amounts. Additionally, major amounts of an unidentified aminolipid and two unidentified lipids not containing a phosphate group, an amino group or a sugar residue were detected. The genomic G+C content of strain 96_Hippo_TS_3/13T was 30 mol%. Based on genotypic, chemotaxonomic and physiological characterizations we propose a novel species of the genus Mesonia, Mesonia hippocampi sp. nov., with strain 96_Hippo_TS_3/13T ( = CIP 110839T = LMG 28572T = CCM 8557T) as the type strain. An emended description of the genus Mesonia is also provided.
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