- Volume 65, Issue Pt_2, 2015
Volume 65, Issue Pt_2, 2015
- New Taxa
-
- Proteobacteria
-
-
Tahibacter caeni sp. nov., isolated from activated sludge
More LessA Gram-reaction-negative, aerobic, rod-shaped, non-spore-forming, non-motile bacterial strain, designated BUT-6T, was isolated from activated sludge of a wastewater-treatment facility. The strain grew at 15–35 °C (optimum 30 °C), pH 4.0–10.0 (optimum pH 7.0) and 0–3.0 % (w/v) NaCl (optimum 1.0 %). Phylogenetic analysis based on 16S rRNA sequences showed that strain BUT-6T was most closely related to Tahibacter aquaticus PYM5-11T (98.6 % similarity). However, the DNA–DNA relatedness between strain BUT-6T and T. aquaticus PYM5-11T was 47.1 %. The major fatty acids (>10 % of total fatty acids) of strain BUT-6T were iso-C15 : 0, iso-C17 : 1ω9c and iso-C17 : 0. The major respiratory quinone was ubiquinone Q-8. The profile of polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, an unidentified aminophospholipid, three unknown aminolipids and unidentified phospholipids. The DNA G+C content of strain BUT-6T was 71.7 mol%. On the basis of the data from the polyphasic taxonomic study presented, strain BUT-6T is considered to represent a novel species of the genus Tahibacter , for which the name Tahibacter caeni sp. nov. is proposed. The type strain is BUT-6T ( = CCTCC AB 2013266T = KACC 17139T).
-
-
-
Falsirhodobacter deserti sp. nov., isolated from sandy soil
More LessA Gram-stain-negative, non-motile, heterotrophic, rod-shaped bacterium, designated strain W402T, was isolated from an enrichment culture of a Tamarix ramosissima rhizosphere soil sample from the Xinjiang desert in the PR China. Analysis of the almost-complete 16S rRNA gene sequence showed that the isolate was phylogenetically related to a species of the genus Falsirhodobacter , having a close relationship to Falsirhodobacter halotolerans JA744T (97 % similarity). However, DNA–DNA relatedness between W402T and F. halotolerans JA744T was 43.2±1.2 %. Strain W402T grew in 0–10 % (w/v) NaCl. The temperature and pH ranges for growth were 10–40 °C and pH 5.5–10.0, respectively. Optimal growth occurred at 1–3 % (w/v) NaCl, 30 °C and pH 7.0. The predominant cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c, 61.4 %), C18 : 0 (9.8 %) and 11-methyl C18 : 1ω7c (8.2 %). The major quinone of strain W402T was Q-10. Phosphatidylethanolamine was predominant in the polar lipid profile. The DNA G+C content of strain W402T was 67.3 mol%. Taken together, these results confirm that W402T represents a novel species of the genus Falsirhodobacter , for which the name Falsirhodobacter deserti sp. nov. is proposed. The type strain is W402T ( = ACCC 05851T = KCTC 32408T).
-
-
-
Novosphingobium marinum sp. nov., isolated from seawater
More LessA Gram-stain-negative, aerobic, short rod-shaped bacterium, strain LA53T, was isolated from a deep-sea water sample collected from the eastern Pacific Ocean. Strain LA53T grew in the presence of 0–7.0 % (w/v) NaCl and at 15-37 °C; optimum growth was observed with 1.0–2.0 % (w/v) NaCl and at 35 °C. Chemotaxonomic analysis showed ubiquinone-10 as the predominant respiratory quinone, C18 : 1ω7c and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) as major fatty acids, and diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid as major polar lipids. The genomic DNA G+C content was 57.7 mol%. Phylogenetic analyses revealed that strain LA53T belongs to the genus Novosphingobium . 16S rRNA gene sequence similarities between strain LA53T and the type strains of species of the genus Novosphingobium with validly published names ranged from 93.1 to 96.3 %. In addition, strain LA53T could be differentiated from Novosphingobium pentaromativorans DSM 17173T and Novosphingobium indicum DSM 23608T as well as the type strain of the type species of the genus, Novosphingobium capsulatum DSM 30196T, by some phenotypic characteristics, including hydrolysis of substrates, utilization of carbon sources and susceptibility to antibiotics. On the basis of phenotypic and genotypic data, strain LA53T represents a novel species within the genus Novosphingobium , for which the name Novosphingobium marinum sp. nov. is proposed. The type strain is LA53T ( = CGMCC 1.12918T = JCM 30307T).
-
-
-
Loktanella sediminum sp. nov., isolated from marine surface sediment
More LessA Gram-staining-negative, strictly aerobic and short rod-shaped bacterium, designated strain S3B03T, was isolated from the sediment of the northern Okinawa Trough. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S3B03T belonged to the genus Loktanella (family Rhodobacteraceae ) and showed the highest sequence similarity with Loktanella litorea KCTC 23883T (96.16 %) and 92.99–95.90 % 16S rRNA gene sequence similarity to other members of the genus Loktanella . Optimal growth occurred in the presence of 2–5 % (w/v) NaCl at pH 7.0–8.0 and 28–32 °C. Ubiquinone-10 (Q-10) was the predominant respiratory quinone. The major fatty acids (>10 % of the total fatty acids) were C18 : 1ω7c and/or C18 : 1ω6c. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and two unidentified polar lipids. The DNA G+C content of strain S3B03T was 57.6 mol%. On the basis of polyphasic analysis, strain S3B03T is considered to represent a novel species of the genus Loktanella , for which the name Loktanella sediminum sp. nov. is proposed. The type strain is S3B03T ( = JCM 30120T = DSM 28715T = MCCC 1K00257T).
-
- Bacteroidetes
-
-
Phenylobacterium kunshanense sp. nov., isolated from the sludge of a pesticide manufacturing factory
More LessA novel aerobic, Gram-stain-negative, motile bacterium, designated strain BUT-10T, was isolated from the sludge of a pesticide manufacturing factory in Kunshan, China. Cells were rod-shaped (0.4–0.45×0.9–1.4 µm) and colonies were white, circular with entire edges and had a smooth surface. The strain grew at 25–37 °C, at pH 6.0–8.0 and with 0–0.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain BUT-10T was a member of the genus Phenylobacterium , and showed highest sequence similarities to Phenylobacterium muchangponense A8T (97.49 %), Phenylobacterium immobile DSM 1986T (97.14 %) and Phenylobacterium lituiforme FaiI3T (96.34 %). Major fatty acids (>5 %) were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). The major isoprenoid quinone was ubiquinone-10. The DNA G+C content was 71.85 mol%. Strain BUT-10T showed low DNA–DNA relatedness with P. muchangponense A8T (15.7±2.9 %) and P. immobile DSM 1986T (12.8±1.1 %). On the basis of the phenotypic, phylogenetic and genotypic data, strain BUT-10T is considered to represent a novel species of the genus Phenylobacterium , for which the name Phenylobacterium kunshanense sp. nov. is proposed. The type strain is BUT-10T ( = CCTCC AB 2013085T = KCTC 42014T).
-
-
-
Hanstruepera neustonica gen. nov., sp. nov., a zeaxanthin-producing member of the family Flavobacteriaceae isolated from estuarine water, and emendation of Sediminibacter furfurosus Khan et al. 2007 emend. Kwon et al. 2014, Mangrovimonas yunxiaonensis Li et al. 2013, Antarcticimonas flava Yang et al. 2009 and Hoppeia youngheungensis Kwon et al. 2014
A Gram-staining-negative, strictly aerobic, yellowish-orange, flexirubin-positive, rod-shaped, non-flagellated, non-spore-forming and non-gliding marine bacterium, designated strain CC-PY-50T, was isolated from estuarine water off Pingtung, Taiwan. The strain produced zeaxanthin as a major carotenoid pigment, and showed highest pairwise 16S rRNA gene sequence similarity to Bizionia hallyeonensis T-y7T (93.9 %) followed by Corallibacter vietnamensis KMM 6217T (93.8 %), Geojedonia litorea YCS-16T (93.7 %) and other members of the family Flavobacteriaceae (<93.7 %). Strain CC-PY-50T established a distinct phyletic lineage associated with Mangrovimonas yunxiaonensis LYYY01T (93.1 % sequence similarity) with poor bootstrap support during neighbour-joining and maximum-likelihood phylogenetic analyses (37 % for each). The polar lipid profile of strain CC-PY-50T was determined to accommodate large numbers of unknown lipids including major amounts of three unidentified aminolipids and two unidentified lipids, and moderate amounts of an unidentified phospholipid, an unidentified glycolipid and an unidentified lipid. In addition, phosphatidylethanolamine was also detected in significant amounts. The major (>5 % of total) fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, C16 : 0 and C16 : 1ω6c and/or C16 : 1ω7c. The DNA G+C content was 37.1 mol% and menaquinone-6 (MK-6) was the sole respiratory quinone. Based on the phylogenetic evidence and several distinguishing phenotypic and chemotaxonomic features, strain CC-PY-50T is proposed to represent a novel genus and species of the family Flavobacteriaceae , for which the name Hanstruepera neustonica gen. nov., sp. nov. is proposed. The type strain of the type species Hanstruepera neustonica gen. nov., sp. nov. is CC-PY-50T ( = JCM 19743T = BCRC 80747T). Emended descriptions of the species Sediminibacter furfurosus , Mangrovimonas yunxiaonensis , Antarcticimonas flava and Hoppeia youngheungensis are also proposed.
-
-
-
Flavobacterium suzhouense sp. nov., isolated from farmland river sludge
More LessA Gram-stain-negative bacterium, designated XIN-1T, was isolated from a farmland river sludge sample in Suzhou, China. Cells of strain XIN-1T were strictly aerobic, non-motile and rod-shaped. Strain XIN-1T grew optimally at pH 7.0 and 28 °C. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain XIN-1T was most closely related to Flavobacterium hauense BX12T (98.2 % sequence similarity), followed by Flavobacterium beibuense F44-8T (96.3 %). The major respiratory quinone was menaquinone-6 and the major polar lipid was phosphatidylethanolamine. The major fatty acids (>5 %) were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), summed feature 4 (comprising iso-C17 : 1 I and/or anteiso-C17 : 1 B), iso-C15 : 0, C16 : 0 and iso-C17 : 0 3-OH. The genomic DNA G+C content of strain XIN-1T was 39.8 mol%. Strain XIN-1T showed low DNA–DNA relatedness with F. hauense BX12T (38.7±0.5 %). On the basis of genotypic and phenotypic data, strain XIN-1T is considered to represent a novel species of the genus Flavobacterium , for which the name Flavobacterium suzhouense sp. nov. is proposed. The type strain is XIN-1T ( = CCTCC AB 2014200T = KCTC 42107T).
-
-
-
Litoribaculum gwangyangense gen. nov., sp. nov., isolated from a sea-tidal flat sediment
More LessA Gram-stain-negative, strictly aerobic bacterium, designated strain GY12T, was isolated from a tidal flat of South Korea. Cells were moderately halotolerant, catalase- and oxidase-positive rods with gliding motility, and were devoid of flagella. Growth of strain GY12T was observed at 15–40 °C (optimum 25–30 °C), pH 6.0–8.5 (optimum pH 7.0–7.5) and with 1–5 % (w/v) NaCl (optimum 1–2 %). The major cellular fatty acids were iso-C15 : 1 G, iso-C15 : 0 and iso-C15 : 0 3-OH. The polar lipids consisted almost entirely of phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The G+C content of the genomic DNA was 34.8 mol% and the only respiratory quinone detected was menaquinone-6 (MK-6). Strain GY12T was most closely related to the genera Gaetbulibacter , Flaviramulus , Mariniflexile and Tamlana with 16S rRNA gene sequence similarities of 94–97 %, but phylogenetic inferences based on 16S rRNA gene sequences showed that the strain formed phyletic lineages distinct from these genera within the family Flavobacteriaceae . On the basis of phenotypic and molecular features, strain GY12T represents a novel species of a new genus in the family Flavobacteriaceae , for which the name Litoribaculum gwangyangense gen. nov., sp. nov. is proposed. The type strain of the type species is GY12T ( = KACC 16441T = JCM 18325T).
-
-
-
Muriicola marianensis sp. nov., isolated from seawater
More LessA Gram-stain-negative, aerobic, orange-pigmented, rod-shaped and non-motile bacterium, designated strain A6B8T, was isolated from seawater of the Mariana Trench. The isolate grew at 4–50 °C (optimum 30–35 °C), at pH 6.5–8.0 (optimum pH 7.5) and with 0.5–4.0 % (w/v) NaCl (optimum 1.0–2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A6B8T was related most closely to the genus Muriicola and shared highest sequence similarity of 97.7 % with Muriicola jejuensis EM44T. Chemotaxonomic analysis showed menaquinone 6 (MK-6) was the predominant isoprenoid and iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH were the major cellular fatty acids. The polar lipid profile of strain A6B8T included phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 47.1 mol%. The DNA–DNA relatedness value (23.3 %) clearly demonstrated that strains A6B8T and M. jejuensis EM44T were representatives of two different species. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, A6B8T ( = CGMCC 1.12606T = KCTC 32436T) is considered to be the type strain of a novel species of the genus Muriicola , for which the name Muriicola marianensis sp. nov. is proposed.
-
-
-
Chitinophaga longshanensis sp. nov., a mineral-weathering bacterium isolated from weathered rock
More LessA Gram-stain-negative, aerobic, yellow-pigmented, non-motile, non-spore-forming, rod-shaped bacterial strain, Z29T, was isolated from the surface of weathered rock (potassic trachyte) from Nanjing, Jiangsu Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain Z29T belongs to the genus Chitinophaga in the family Chitinophagaceae . Levels of 16S rRNA gene sequence similarity between strain Z29T and the type strains of recognized species of the genus Chitinophaga ranged from 92.7 to 98.2 %. The main fatty acids of strain Z29T were iso-C15 : 0, C16 : 1ω5c and iso-C17 : 0 3-OH. It also contained menaquinone 7 (MK-7) as the respiratory quinone and homospermidine as the main polyamine. The polar lipid profile contained phosphatidylethanolamine, unknown aminolipids, unknown phospholipids and unknown lipids. The total DNA G+C content of strain Z29T was 51.3 mol%. Phenotypic properties and chemotaxonomic data supported the affiliation of strain Z29T with the genus Chitinophaga . The low level of DNA–DNA relatedness (ranging from 14.6 to 29.8 %) to the type strains of other species of the genus Chitinophaga and differential phenotypic properties demonstrated that strain Z29T represents a novel species of the genus Chitinophaga , for which the name Chitinophaga longshanensis sp. nov. is proposed. The type strain is Z29T ( = CCTCC AB 2014066T = LMG 28237T).
-
-
-
Marivirga lumbricoides sp. nov., a marine bacterium isolated from the South China Sea
More LessA novel, aerobic, heterotrophic, orange-pigmented, Gram-staining-negative, rod-shaped, gliding bacterial strain, designated JLT2000T, was isolated from surface water of the South China Sea. The strain was oxidase- and catalase-positive. The major cellular fatty acids of strain JLT2000 T were C12 : 0, iso-C15 : 1 G, iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 0. MK-7 was the major respiratory quinone and the major polar lipids were phosphatidylcholine and phosphatidylethanolamine. The genomic DNA G+C content of strain JLT2000T was 37.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JLT2000T formed a branch within the genus Marivirga , but was clearly separated from the two established species of this genus, Marivirga tractuosa and Marivirga sericea . The 16S rRNA gene sequence similarity of strain JLT2000T with the type strains of these two species was 95.8 % and 96.1 %, respectively. Strain JLT2000T had a shorter cell length and wider growth range in different temperatures and salinities than those of Marivirga tractuosa NBRC 15989T and Marivirga sericea NBRC 15983T. In addition, strain JLT2000T could utilize more carbon sources and hydrolyse more polymers than Marivirga tractuosa NBRC 15989T and Marivirga sericea NBRC 15983T. Based on this polyphasic analysis, strain JLT2000T represents a novel species of the genus Marivirga , for which the name Marivirga lumbricoides sp. nov. is proposed. The type strain is JLT2000T ( = JCM 18012T = CGMCC 1.10832T).
-
-
-
Belliella kenyensis sp. nov., isolated from an alkaline lake
A red-pigmented, Gram-reaction-negative, aerobic bacterial strain, designated No.164T, was isolated from sediment sample from the alkaline Lake Elmenteita located in the Kenyan Rift Valley. Results of 16S rRNA gene sequence analysis indicated that the isolate represented a member of the genus Belliella , with the highest sequence similarity (97 %) to Belliella pelovolcani DSM 46698T. Optimal growth temperature was 30–35 °C, at pH 7.0–12.0 in the presence of 0–4 % (w/v) NaCl. Flexirubins were absent. The respiratory menaquinone (MK-7), predominant cellular fatty acids (iso-C15 : 0, anteiso-C15 : 0 and a mixture of C16 : 1ω7c and/or iso-C15 : 0 2-OH) and DNA G+C content (38.1 mol%) of strain No.164T were consistent with those of other members of the genus Belliella . The polar lipids consisted of phosphatidylethanolamine, eight unspecified lipids and one unspecified phospholipid. Several phenotypic characteristics can be used to differentiate this isolate from those of other species of the genus Belliella . The results of polyphasic analyses presented in this study indicated that this isolate should be classified as representing a novel species of the genus Belliella . The name Belliella kenyensis sp. nov. is therefore proposed; the type strain is strain No.164T ( = DSM 46651T = CECT 8551T).
-
-
-
Lutibacter oricola sp. nov., a marine bacterium isolated from seawater
More LessA bacterial strain, UDC377T, was isolated from seawater samples collected at Seo-do on the coast of Dokdo island in the East Sea, and was subjected to taxonomic study using a polyphasic approach. Strain UDC377T was pale-yellow, Gram-staining-negative, non-motile, rod-shaped and aerobic. The strain grew optimally at 25–28 °C, in the presence of 2 % (w/v) NaCl and at pH 7.0–7.5. Strain UDC377T produced carotenoid pigments; however, it did not produce flexirubin-type pigments. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain UDC377T clustered with members of the genus Lutibacter and appeared most closely related to Lutibacter agarilyticus KYW566T (96.0 % 16S rRNA gene sequence similarity) followed by L. aestuarii MA-My1T (95.0 %), L. litoralis CL-TF09T (94.9 %), L. maritimus S7-2T (94.1 %) and L. flavus IMCC1507T (94.0 %). The DNA G+C content of strain UDC377T was 30.8 mol%. Strain UDC377T contained MK-6 as the predominant menaquinone, iso-C15 : 0 3-OH, iso-C15 : 0 and iso-C16 : 0 3-OH as the major fatty acids, and phosphatidylethanolamine, two unknown aminolipids and six unknown lipids as the major polar lipids. Based on phenotypic properties and phylogenetic data presented, strain UDC377T is considered to represent a novel species of the genus Lutibacter , for which the name Lutibacter oricola sp. nov. is proposed. The type strain is UDC377T ( = DSM 24956T = KCTC 23668T).
-
-
-
Arachidicoccus rhizosphaerae gen. nov., sp. nov., a plant-growth-promoting bacterium in the family Chitinophagaceae isolated from rhizosphere soil
Three novel bacterial strains, designated Vu-144T, Vu-7 and Vu-35, were isolated on minimal medium from rhizosphere soil of field-grown cowpea and subjected to a taxonomic study using a polyphasic approach. Cells of the strains were Gram-stain-negative, non-motile, non-spore-forming, coccoid rods, and formed non-pigmented colonies. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Vu-144T was affiliated with an uncultivated lineage of the phylum Bacteroidetes . Its closest phylogenetic neighbour was the recently described species Niastella populi , a member of the family Chitinophagaceae , with just 90.7 % sequence similarity to the type strain. The only isoprenoid quinone detected was menaquinone 7 (MK-7). The fatty acid profiles showed large amounts of iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G and minor amounts of summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and other fatty acids, allowing the differentiation of the strains from other genera. The G+C content of the genomic DNA of the three strains ranged from 43.1 to 44.3 mol%. In addition to phosphatidylethanolamine, the major polar lipids were three unidentified aminophospholipids (APL1–APL3), two unidentified phospholipids (PL1, PL2) and three unidentified lipids (UL1–UL3). Biochemical test patterns also differed from those of Niastella populi and members of other genera. All three isolates showed plant-growth-promoting properties, e.g. the ability to produce indole-3-acetic acid and NH3 and to solubilize phosphate, utilized 1-aminocyclopropane 1-carboxylate (ACC) as a sole source of nitrogen and possessed the ACC deaminase enzyme. The novel isolates readily colonized roots and stimulated growth of tomato and cowpea under glasshouse conditions. Inoculated plants showed a 45–60 % increase in dry matter weight with respect to uninoculated controls. On the basis of the evidence from our polyphasic study, isolate Vu-144T represents a novel genus and species in the family Chitinophagaceae , for which the name Arachidicoccus rhizosphaerae gen. nov., sp. nov. is proposed. The type strain of Arachidicoccus rhizosphaerae is Vu-144T ( = KCTC 22378T = NCIMB 14473T).
-
-
-
Arcticibacter eurypsychrophilus sp. nov., isolated from ice core
A Gram-stain-negative, rod-shaped, non-flagellated bacterium, strain MJ9-5T, was isolated from ice core of Muji Glacier. Colonies of strain MJ9-5T were pink, convex and round on R2A agar. Strain MJ9-5T grew between −1 to 25 °C with an optimum growth temperature of 10–15 °C. The strain tolerated 0–1.2 % (w/v) NaCl with an optimum of 1 %. The major cellular fatty acids of strain MJ9-5T were iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C1 6 : 1ω7c). The G+C content of the genomic DNA was 38.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ9-5T was related to members of the genus Arcticibacter . On the basis of the phenotypic characteristics and phylogenetic analysis, a novel species of this genus, Arcticibacter eurypsychrophilus sp. nov., is proposed. The type strain is MJ9-5T ( = KCTC 42008T = JCM 19862T).
-
-
-
Flaviramulus aquimarinus sp. nov., isolated from seawater
More LessA yellow-coloured, rod-shaped, non-motile, Gram-stain-negative and facultative anaerobic bacterium, designated KYW499T, was isolated from seawater collected at Suncheon Bay, Republic of Korea. The isolate required sea salts for growth. Flexirubin-type pigments were absent. Major cellular fatty acids (>10 % of total) were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH. The main polar lipids were phosphatidylethanolamine and two unidentified polar lipids. The predominant isoprenoid quinone was MK-6. The genomic DNA G+C content was 31.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KYW499T belonged to the family Flavobacteriaceae , and was related to the genus Flaviramulus . Data from a polyphasic taxonomy study suggested that the isolate represents a novel species of the genus Flaviramulus , for which the name Flaviramulus aquimarinus sp. nov. is proposed. The type strain is KYW499T ( = KCTC 23924T = JCM 18274T).
-
-
-
Dysgonomonas termitidis sp. nov., isolated from the gut of the subterranean termite Reticulitermes speratus
More LessA facultatively anaerobic, Gram-stain-negative, non-motile and rod-shaped bacterium, strain N-10T, was isolated from the gut of the termite Reticulitermes speratus. Strain N-10T was closely related to Dysgonomonas gadei JCM 16698T according to 16S rRNA gene sequence similarity analysis (98 %) and DNA–DNA relatedness value (≤61.3 %). The optimum growth temperature of strain N-10T was 30 °C, which was distinct from that (37 °C) of known species of the genus Dysgonomonas . Growth of strain N-10T was inhibited on medium containing 5 or 20 % bile, unlike other species of the genus Dysgonomonas . In addition, acid production in the API 20A system and enzymic reactions in the Rapid ID 32A system of strain N-10T differed from those of other species of the genus Dysgonomonas . Based on these characteristics, strain N-10T represents a novel species of the genus Dysgonomonas , for which the name Dysgonomonas termitidis sp. nov. is proposed. The type strain is N-10T ( = JCM 30204T = CCUG 66188T).
-
-
-
Vitellibacter nionensis sp. nov., isolated from a shallow water hydrothermal vent
A novel, Gram-stain-negative, non-motile, rod-shaped yellow bacterium, designated VBW088T was isolated from a shallow water hydrothermal vent in Espalamaca in the Azores. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain VBW088T clustered with three type strains of species of the genus Vitellibacter and exhibited a sequence similarity of 97.3 % with Vitellibacter soesokkakensis RSSK-12T. However, strain VBW088T and V. soesokkakensis RSSK-12T exhibited low DNA–DNA relatedness (12.7±3.5 %). Strain VBW088T was positive for catalase and oxidase. Growth occurred at 10–37 °C, with the optimum at 30 °C, and at pH 6.0–8.0 (optimum pH 6.0) and in up to 5 % (w/v) NaCl with optimum growth at 1–2 % (w/v) NaCl. The major fatty acids (>10 %) were iso-C15 : 0 (33.5 %) and iso-C17 : 0 3-OH (32.0 %). The polar lipids detected in strain VBW088T consisted of phosphatidylethanolamine, one unidentified aminolipid and three unidentified phospholipids. The DNA G+C content of strain VBW088T was 36.7 mol%. On the basis of phylogenetic inference, DNA–DNA relatedness, chemotaxonomic analysis and physiological data, the isolate represents a novel species of the genus Vitellibacter , for which the name Vitellibacter nionensis sp. nov. is proposed, with the type strain as VBW088T ( = KCTC 32420T = MCC 2354T).
-
-
-
Mucilaginibacter aquaedulcis sp. nov., isolated from fresh water
More LessAn aerobic, Gram-stain-negative, rod-shaped bacterium, designated strain PGW1-R01T, was isolated from fresh water from the Yeongju in the Republic of Korea. The strain grew optimally at 30 °C and at pH 6-8 on R2A agar. The major cellular fatty acids were summed feature 3 [comprising C16 : 1ω7c and/or C16 : 1ω6c (50.2 %) and iso-C15 : 0 (24.8 %)]. The major respiratory quinone was MK-7. The G+C contents were 39.4 mol% and the predominant respiratory quinone was MK-7. Based on 16S rRNA gene phylogeny, the strain belongs to the genus Mucilaginibacter . The strain PGW1-R01T was closely related to ‘ Mucilaginibacter ginsenosidivorax’ (96.6 % sequence similarity), Mucilaginibacter lappiensis (96.4 %) and Mucilaginibacter flavus (96.4 %). On the basis of the evidence presented in this study, strain PGW1-R01T represents a novel species of the genus Mucilaginibacter , for which the name Mucilaginibacter http://dx.doi.org/10.1601/nm.11437 aquaedulcis sp. nov., is proposed. The type strain is PGW1-R01T( = KCTC 23942T = CECT 8102T).
-
- Other Bacteria
-
-
Deinococcus antarcticus sp. nov., isolated from soil
More LessA pink-pigmented, non-motile, coccoid bacterial strain, designated G3-6-20T, was isolated from a soil sample collected in the Grove Mountains, East Antarctica. This strain was resistant to UV irradiation (810 J m−2) and slightly more sensitive to desiccation as compared with Deinococcus radiodurans . Phylogenetic analyses based on the 16S rRNA gene sequence of the isolate indicated that the organism belongs to the genus Deinococcus . Highest sequence similarities were with Deinococcus ficus CC-FR2-10T (93.5 %), Deinococcus xinjiangensis X-82T (92.8 %), Deinococcus indicus Wt/1aT (92.5 %), Deinococcus daejeonensis MJ27T (92.3 %), Deinococcus wulumuqiensis R-12T (92.3 %), Deinococcus aquaticus PB314T (92.2 %) and Deinococcus radiodurans DSM 20539T (92.2 %). Major fatty acids were C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), anteiso-C15 : 0 and C16 : 0. The G+C content of the genomic DNA of strain G3-6-20T was 63.1 mol%. Menaquinone 8 (MK-8) was the predominant respiratory quinone. Based on its phylogenetic position, and chemotaxonomic and phenotypic characteristics, strain G3-6-20T represents a novel species of the genus Deinococcus , for which the name Deinococcus antarcticus sp. nov. is proposed. The type strain is G3-6-20T ( = DSM 27864T = CCTCC AB 2013263T).
-
Volumes and issues
-
Volume 74 (2024)
-
Volume 73 (2023)
-
Volume 72 (2022 - 2023)
-
Volume 71 (2020 - 2021)
-
Volume 70 (2020)
-
Volume 69 (2019)
-
Volume 68 (2018)
-
Volume 67 (2017)
-
Volume 66 (2016)
-
Volume 65 (2015)
-
Volume 64 (2014)
-
Volume 63 (2013)
-
Volume 62 (2012)
-
Volume 61 (2011)
-
Volume 60 (2010)
-
Volume 59 (2009)
-
Volume 58 (2008)
-
Volume 57 (2007)
-
Volume 56 (2006)
-
Volume 55 (2005)
-
Volume 54 (2004)
-
Volume 53 (2003)
-
Volume 52 (2002)
-
Volume 51 (2001)
-
Volume 50 (2000)
-
Volume 49 (1999)
-
Volume 48 (1998)
-
Volume 47 (1997)
-
Volume 46 (1996)
-
Volume 45 (1995)
-
Volume 44 (1994)
-
Volume 43 (1993)
-
Volume 42 (1992)
-
Volume 41 (1991)
-
Volume 40 (1990)
-
Volume 39 (1989)
-
Volume 38 (1988)
-
Volume 37 (1987)
-
Volume 36 (1986)
-
Volume 35 (1985)
-
Volume 34 (1984)
-
Volume 33 (1983)
-
Volume 32 (1982)
-
Volume 31 (1981)
-
Volume 30 (1980)
-
Volume 29 (1979)
-
Volume 28 (1978)
-
Volume 27 (1977)
-
Volume 26 (1976)
-
Volume 25 (1975)
-
Volume 24 (1974)
-
Volume 23 (1973)
-
Volume 22 (1972)
-
Volume 21 (1971)
-
Volume 20 (1970)
-
Volume 19 (1969)
-
Volume 18 (1968)
-
Volume 17 (1967)
-
Volume 16 (1966)
-
Volume 15 (1965)
-
Volume 14 (1964)
-
Volume 13 (1963)
-
Volume 12 (1962)
-
Volume 11 (1961)
-
Volume 10 (1960)
-
Volume 9 (1959)
-
Volume 8 (1958)
-
Volume 7 (1957)
-
Volume 6 (1956)
-
Volume 5 (1955)
-
Volume 4 (1954)
-
Volume 3 (1953)
-
Volume 2 (1952)
-
Volume 1 (1951)