- Volume 64, Issue Pt_7, 2014
Volume 64, Issue Pt_7, 2014
- New Taxa
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- Proteobacteria
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Paludibacterium paludis sp. nov., isolated from a marsh
More LessA bacterial strain, designated KBP-21T, was isolated from a water sample taken from the Banping Lake Wetland Park in Taiwan and characterized in a taxonomic study using a polyphasic approach. Cells of strain KBP-21T were Gram-stain-negative, facultatively anaerobic, poly-β-hydroxybutyrate-accumulating, motile rods that formed yellow colonies. Growth occurred at 15–40 °C (optimum, 30 °C), at pH 5.0–8.0 (optimum, pH 8.0) and with 0–2 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KBP-21T belonged to the genus Paludibacterium within the family Neisseriaceae of the class Betaproteobacteria and the closest related neighbour was Paludibacterium yongneupense 5YN8-15T with a 16S rRNA gene sequence similarity value of 96.4 %. Strain KBP-21T contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω7c as the predominant fatty acids. The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 62.1 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one uncharacterized aminophospholipid and several uncharacterized phospholipids. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain KBP-21T represents a novel species in the genus Paludibacterium , for which the name Paludibacterium paludis sp. nov. is proposed. The type strain is KBP-21T ( = BCRC 80514T = LMG 27230T = KCTC 32182T).
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Reyranella graminifolii sp. nov., isolated from bamboo (Phyllostachys bambusoides) litter
More LessA novel strain designated Wo-34T was isolated from bamboo (Phyllostachys bambusoides) litter. Cells were Gram-stain-negative, non-motile, catalase-negative and oxidase-positive rods. The isolate grew aerobically at 15–35 °C (optimum 28 °C), pH 4.0–9.0 (optimum pH 7.0) and in the presence of 0–1.0 % (w/v) NaCl (optimum 0.1 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain Wo-34T belonged to the genus Reyranella with the sequence similarities of 97.9 % and 97.3 % to the other species of the genus Reyranella , Reyranella massiliensis 521T and Reyranella soli KIS14-15T, respectively. The predominant ubiquinone was Q-10. Major fatty acids were C18 : 1ω7c, C18 : 1 2-OH and C19 : 0 cyclo ω8c. The polar lipids contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown aminolipid, unidentified phospholipids and unknown lipids. DNA–DNA relatedness values between strain Wo-34T and R. massiliensis DSM 23428T and R. soli KACC 13034T were 35 % and 29 %, respectively. On the basis of polyphasic analysis from this study, strain Wo-34T represents a novel species of the genus Reyranella for which the name Reyranella graminifolii sp. nov. is proposed. The type strain is Wo-34T ( = KACC 17605T = NBRC 109813T).
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- Bacteroidetes
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Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans
More LessA Gram-stain-negative, facultatively anaerobic, gliding, yellow-pigmented bacterium, designated SS12T, was isolated from shark gill homogenate and characterized using a polyphasic approach. The strain was catalase-positive and oxidase-negative. Optimal growth occurred at 28–30 °C, pH 7.0–7.5 and in the presence of 2–4 % (w/v) NaCl. The DNA G+C content was 40.0 mol%. The strain contained MK-7 as the prevailing menaquinone; iso-C15 : 0 and anteiso-C15 : 0 as the major cellular fatty acids; and phosphatidylethanolamine and an unknown lipid as the predominant polar lipids. Comparative analysis of 16S rRNA gene sequences demonstrated that the novel isolate showed the highest sequence similarity (94.68 %) to Saccharicrinis fermentans DSM 9555T and the sequence similarities among the type strains of all other species studied were less than 92 %. A phylogenetic tree, based on 16S rRNA gene sequences, showed that strain SS12T and Saccharicrinis fermentans DSM 9555T formed a distinct cluster within the family Marinilabiliaceae . On the basis of its phylogenetic position and phenotypic traits, strain SS12T represents a novel species of genus Saccharicrinis , for which the name Saccharicrinis carchari sp. nov. is proposed. The type strain is SS12T ( = CICC 10590T = DSM 27040T). Emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans are also provided.
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Mucilaginibacter pineti sp. nov., isolated from Pinus pinaster wood from a mixed grove of pines trees
Bacterial strain M47C3BT was isolated from the endophytic microbial community of a Pinus pinaster tree branch from a mixed grove of pines. Phylogenetic analysis of 16S rRNA gene sequences showed that this organism represented one distinct branch within the family Sphingobacteriaceae , most closely related to the genus Mucilaginibacter . Strain M47C3BT formed a distinct lineage, closely related to Mucilaginibacter dorajii KACC 14556T, with which it shared 97.2 % 16S rRNA gene sequence similarity. The other members of the genus Mucilaginibacter included in the same clade were Mucilaginibacter lappiensis ATCC BAA-1855T sharing 97.0 % similarity and Mucilaginibacter composti TR6-03T that had a lower similarity (95.7 %). The novel strain was Gram-staining-negative, formed rod-shaped cells, grew optimally at 26 °C and at pH 7, and was able to grow with up to 0.3 % (w/v) NaCl. The respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids of the strain were summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH), iso-C15 : 0 and iso-C17 : 0 3-OH, representing 73.5 % of the total fatty acids. The major components of the polar lipid profile of strain M47C3BT consisted of phosphatidylethanolamine, three unidentified aminophospholipids, one unidentified aminolipid and three unidentified polar lipids. The G+C content of the DNA was 40.6 mol%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics we propose the name Mucilaginibacter pineti sp. nov. for the novel species represented by strain M47C3BT ( = CIP 110632T = LMG 28160T).
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Arcticibacter pallidicorallinus sp. nov. isolated from glacier ice
More LessA Gram-stain-negative, rod-shaped bacterium (strain Hh36T) was isolated from the No. 1 glacier in Xinjiang, north-west China. Colonies of strain Hh36T were pink, convex and round on PYG medium plates. Strain Hh36T was able to grow at 4–30 °C and pH 6.0–8.0. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Hh36T was related to members of the genus Arcticibacter . The major cellular fatty acids of the novel strain were iso-C15 : 0, summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) and iso-C17 : 0 3-OH. The G+C content of the genomic DNA was 44.0 mol%. On the basis of phenotypic characteristics and phylogenetic analysis, strain Hh36T is considered to represent a novel species of the genus Arcticibacter , for which the name Arcticibacter pallidicorallinus sp. nov. is proposed. The type strain is Hh36T ( = CGMCC 1.9313T = KCTC 32542T).
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Spirosoma arcticum sp. nov., isolated from high Arctic glacial till
More LessA novel orange-pigmented strain, designated R2-35T, was isolated from a glacier till near Ny-Alesund, Svalbard Archipelago, Norway. The cells were aerobic, Gram-negative, rod-shaped and sometimes filamentous. Growth occurred at 4–28 °C (optimum, 20 °C), at pH 7.0–9.0 (optimum, pH 8.0) and with 0–1 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R2-35T belonged to the genus Spirosoma with sequence similarity to related species ranging from 91.65 to 95.19 %. Strain R2-35T contained C16 : 0 (10.7 %), C18 : 0 (9.2 %), C16 : 1ω5c (16.5 %) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c) (24.6 %) as the major cellular fatty acids, MK-7 as the major respiratory quinone, and phosphatidylethanolamine as the main polar lipid. The DNA G+C content of strain R2-35T was 54.9 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain R2-35T is considered to represent a novel species of the genus Spirosoma , for which the name Spirosoma arcticum sp. nov., is proposed, The type strain is R2-35T ( = CCTCC AB 2012849T = LMG 28141T).
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Mucilaginibacter koreensis sp. nov., isolated from leaf mould
More LessA Gram-staining-negative, strictly aerobic, rod-shaped, pale-pink pigmented bacterial strain, designated TF8T, was isolated from leaf mould in Cheonan, Republic of Korea. Its taxonomic position was determined through a polyphasic approach. Optimal growth occurred on R2A agar without NaCl supplementation, at 25–28 °C and at pH 6.0–7.0. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TF8T belongs to the genus Mucilaginibacter in the family Sphingobacteriaceae . The sequence similarity between 16S rRNA genes of strain TF8T and the type strains of other species of the genus Mucilaginibacter ranged from 92.1 to 94.7 %. The closest relatives of strain TF8T were Mucilaginibacter lutimaris BR-3T (94.7 %), M. soli R9-65T (94.5 %), M. litoreus BR-18T (94.5 %), M. rigui WPCB133T (94.0 %) and M. daejeonensis Jip 10T (93.8 %). The major isoprenoid quinone was MK-7 and the major cellular fatty acids were iso-C15 : 0 (33.0 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 24.8 %) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 13.0 %). The major polar lipids of TF8T were phosphatidylethanolamine and three unidentified aminophospholipids. The G+C content of the genomic DNA was 46.2 mol%. On the basis of the data presented here, strain TF8T is considered to represent a novel species of the genus Mucilaginibacter , for which the name Mucilaginibacter koreensis sp. nov. is proposed. The type strain is TF8T ( = KACC 17468T = JCM 19323T).
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Pedobacter huanghensis sp. nov. and Pedobacter glacialis sp. nov., isolated from Arctic glacier foreland
More LessTwo psychrotolerant, Gram-stain-negative, rod-shaped bacterial strains, designed M1-27T and 8-24T, were subjected to polyphasic taxonomic studies. Strain M1-27T was isolated from the foreland of the Midtre Lovénbreen glacier, whereas strain 8-24T was isolated from the foreland of the Austre Lovénbreen glacier. Both were Arctic glacier forelands, near Ny-Ålesund, Svalbard Archipelago, Norway. Strains M1-27T and 8-24T exhibited 16S rRNA gene sequence similarities of 91.0–96.0 % and 92.3–96.7 %, respectively, to type strains of recognized species of the genus Pedobacter . Phylogenetic analysis based on 16S rRNA gene sequences showed that the two strains were grouped with members of the genus Pedobacter, but represented distinct taxa. Both strains contained MK-7 as the predominant menaquinone. The DNA G+C contents of strains M1-27T and 8-24T were 43.8 % and 39.4 %, respectively. The phenotypic characteristics, biochemical properties and polygenetic analysis, clearly indicated that strains M1-27T ( = CCTCC AB 2012936T = LMG 28205T) and 8-24T ( = CCTCC AB 2012941T = NRRL B-59993T) represent two novel species of the genus Pedobacter , for which the names Pedobacter huanghensis sp. nov. and Pedobacter glacialis sp. nov., respectively, are proposed.
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- Eukaryotic Micro-organisms
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Survey on diversity of marine/saline anaerobic Heterolobosea (Excavata: Discoba) with description of seven new species
More LessDiversity of the anaerobic Heterolobosea (Excavata: Discoba) is only poorly understood, especially in marine environments. We have isolated and cultured 16 strains of anaerobic heteroloboseid amoebae and flagellates from brackish, marine and saline anoxic habitats worldwide. Phylogenetic analyses of SSU rDNA sequences and light-microscopic observations showed that all the strains belong to the family Psalteriomonadidae, the main anaerobic lineage of Heterolobosea, and that they represent eight species from the genera Monopylocystis, Harpagon and Pseudoharpagon. Seven species are newly isolated and described here as Monopylocystis minor n. sp., Monopylocystis robusta n. sp., Monopylocystis elegans n. sp., Monopylocystis disparata n. sp., Harpagon salinus n. sp., Pseudoharpagon longus n. sp. and Pseudoharpagon tertius n. sp. Amoebae, cysts and the ultrastructure of the genus Pseudoharpagon are presented for the first time.
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Hanseniaspora nectarophila sp. nov., a yeast species isolated from ephemeral flowers
More LessSeven apiculate yeast strains that were isolated from the flowers of Syphocampylus corymbiferus Pohl in Brazil are genetically, morphologically and phenotypically distinct from recognized species of the genera Hanseniaspora and Kloeckera. Genetic discontinuities between the novel strains and their closest relatives were found using a networking approach based on the concatenated sequences of the rRNA gene (internal transcribed spacer and D1/D2 of the LSU), and the protein-coding genes for actin and translation elongation factor-1α. Phylogenetic analysis based on the rRNA and the actin gene placed the novel species represented by the strains in close relationship to Hanseniaspora meyeri and Hanseniaspora clermontiae. PCR fingerprinting with microsatellite primers confirmed the genetic heterogeneity of the novel species. The name Hanseniaspora nectarophila sp. nov. is proposed, with UFMG POG a.1T ( = ZIM 2311T = CBS 13383T) as the type strain; MycoBank no. MB807210. As the current description of the genus does not allow the presence of multilateral budding, an emended diagnosis of the genus Hanseniaspora Zikes is proposed.
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Morphological and molecular characterization of Parafurgasonia zhangi spec. nov. and Chilodonella acuta Kahl, 1931 (Protozoa, Ciliophora), from a soil habitat of Saudi Arabia
More LessThe morphology and infraciliature of two soil ciliates, Parafurgasonia zhangi spec. nov. and Chilodonella acuta Kahl, 1931, collected from Saudi Arabia, were investigated by observations of both living cells and specimens after standard staining methods. P. zhangi differs from its congeners by the combination of the following features: excretory pore quite near posterior end of paroral membrane, 16 or 17 somatic kineties with about 11 kinetids in each one on dorsal side, paroral membrane gently curved and composed of about 15 dikinetids, and hypostomial organelle composed of four or five files of kinetids with four monokinetids each. The diagnosis of Chilodonella acuta was renewed to include characteristics revealed by the silver impregnation method: cells in vivo measuring 33–45×18–26 µm, dorsal hump and tail-like podite present, two contractile vacuoles, seven left and five right kineties, 9–11 nematodesmal rods, and dorsal brush containing about 11 basal bodies. Phylogenetic analyses based on small-subunit rRNA gene sequences showed that P. zhangi was closer to species of the Colpodidiidae rather than the Furgasoniidae represented by Furgasonia blochmanni, and Chilodonella acuta clustered with its congener Chilodonella uncinata but was a well-outlined species of the genus.
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- Errata
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Volumes and issues
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Volume 74 (2024)
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Volume 73 (2023)
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Volume 72 (2022 - 2023)
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Volume 71 (2020 - 2021)
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Volume 70 (2020)
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Volume 69 (2019)
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Volume 68 (2018)
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Volume 67 (2017)
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Volume 66 (2016)
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Volume 65 (2015)
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Volume 64 (2014)
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Volume 63 (2013)
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Volume 62 (2012)
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Volume 61 (2011)
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Volume 60 (2010)
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Volume 59 (2009)
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Volume 58 (2008)
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Volume 57 (2007)
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Volume 56 (2006)
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Volume 55 (2005)
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Volume 54 (2004)
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Volume 53 (2003)
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Volume 52 (2002)
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Volume 51 (2001)
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Volume 50 (2000)
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Volume 49 (1999)
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Volume 48 (1998)
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Volume 47 (1997)
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Volume 46 (1996)
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Volume 45 (1995)
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Volume 44 (1994)
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Volume 43 (1993)
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Volume 42 (1992)
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Volume 41 (1991)
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Volume 40 (1990)
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Volume 39 (1989)
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Volume 38 (1988)
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Volume 37 (1987)
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Volume 36 (1986)
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Volume 35 (1985)
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Volume 34 (1984)
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Volume 33 (1983)
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Volume 32 (1982)
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Volume 31 (1981)
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Volume 30 (1980)
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Volume 29 (1979)
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Volume 28 (1978)
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Volume 27 (1977)
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Volume 26 (1976)
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Volume 25 (1975)
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Volume 24 (1974)
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Volume 23 (1973)
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Volume 22 (1972)
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Volume 21 (1971)
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Volume 20 (1970)
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Volume 19 (1969)
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Volume 18 (1968)
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Volume 17 (1967)
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Volume 16 (1966)
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Volume 15 (1965)
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Volume 14 (1964)
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Volume 13 (1963)
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Volume 12 (1962)
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Volume 11 (1961)
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Volume 10 (1960)
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Volume 9 (1959)
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Volume 8 (1958)
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Volume 7 (1957)
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Volume 6 (1956)
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Volume 5 (1955)
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Volume 4 (1954)
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Volume 3 (1953)
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Volume 2 (1952)
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Volume 1 (1951)