- Volume 62, Issue Pt_3, 2012

A yellow-pigmented, hexachlorocyclohexane (HCH)-degrading bacterium, designated F2T, was isolated from an HCH dumpsite at Ummari village in Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Sphingobium. Its closest neighbour was Sphingobium japonicum UT26T (99.2 % 16S rRNA gene sequence similarity). The DNA G+C content was 65.7 mol%. The polyamine profile showed the presence of spermidine. The respiratory pigment was ubiquinone Q-10. The predominant cellular fatty acids were C16 : 0 (12.5 %), C14 : 0 2-OH (8.1 %), summed feature 3 (consisting of C16 : 1ω7c and/or C16 : 1ω6c; 5.8 %) and summed feature 8 (consisting of C18 : 1ω7c and/or C18 : 1ω6c; 53.1 %). The major polar lipids of strain F2T were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and sphingoglycolipid. DNA–DNA relatedness and biochemical and physiological characters clearly distinguished the isolate from its closest phylogenetic neighbours. Thus, strain F2T represents a novel species of the genus Sphingobium, for which the name Sphingobium lucknowense sp. nov. is proposed. The type strain is strain F2T ( = MTCC 9456T  = CCM 7544T).

A Gram-negative, aerobic, ovoid to rod-shaped bacterial strain, KME 002T was isolated from a marine ascidian, Halocynthia roretzi, off the coast of Gangneung, Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that this strain belonged to the family Rhodobacteraceae in the class Alphaproteobacteria and was closely related to the type strains of Dinoroseobacter shibae, Roseovarius crassostreae and Pseudoruegeria aquimaris with 95.0, 94.7 and 94.5 % 16S rRNA gene sequence similarities, respectively. KME 002T was an obligately halophilic bacterium requiring 1 to 5 % (w/v) NaCl, with an absolute requirement for magnesium chloride for growth. Cells were motile by means of a single polar flagellum and showed budding fission. The predominant cellular fatty acid of the isolate was C18 : 1ω7c and Q-10 was the major ubiquinone. The DNA G+C content of the strain was 71.6 mol%. The major secondary metabolites from cultures in liquid medium were cholic acid derivatives, including 3α,12α-hydroxy-3-keto-glycocholanic acid, 12-hydroxy-3-keto-glycocholanic acid, nutriacholic acid and deoxycholic acid. These characteristics determined in this polyphasic study suggest that strain KME 002T represents a novel species in a new genus of the family Rhodobacteraceae. The name Hasllibacter halocynthiae gen. nov., sp. nov. is proposed for this isolate, and the type strain is KME 002T ( = JCM 16214T = KCCM 90082T).

Three strains of Gram-negative, aerobic, motile bacteria with bipolar flagella were isolated from acidic tundra wetland soils near the city of Vorkuta and from the Chukotka and Yugorsky Peninsulas and designated strains V-022T, Ch-022 and Ju-022. The cells were rod-shaped, 0.5–0.6 µm in width and 1.3–4.5 µm in length and reproduced by irregular fission. These bacteria were facultative methylotrophs that used methanol, methylamines and a wide range of other sources of carbon and energy such as sugars and polysaccharides, ethanol and amino acids. The isolates used the Calvin–Benson pathway for the assimilation of one-carbon compounds and were unable to fix nitrogen. The new strains were moderately acidophilic and psychrotolerant, capable of growth over a pH range of 4.0 to 7.8, with optimum growth at pH 5.5–6.0. Growth occurred between 4 and 30 °C (optimum 20–25 °C). The principal phospholipid fatty acid was C18 : 1ω7c. The DNA G+C content of strain V-022T was 65.2 mol%. Analysis of the 16S rRNA gene sequences revealed that all three isolates V-022T, Ch-022 and Yu-022 exhibited almost identical 16S rRNA gene sequences (99.9 % gene sequence similarity) and formed a new lineage within the class Alphaproteobacteria. The name Methylorosula polaris is suggested to accommodate this new genus and novel species with strain V-022T ( = DSM 22001T = VKM V-2485T) as the type strain of the type species.

Human Psychrobacter isolates, other than Psychrobacter phenylpyruvicus, are predominantly designated Psychrobacter immobilis. Phenotypic and genotypic testing of Psychrobacter isolates that have been deposited in different culture collections as P. immobilis indicates that most of these human isolates belong to the species Psychrobacter faecalis and Psychrobacter pulmonis.

A Gram-staining-negative, aerobic, non-motile and rod-shaped bacterial strain, designated DPG-21T, was isolated from seawater from the South Sea in Korea, and investigated using a polyphasic taxonomic approach. Strain DPG-21T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. In a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain DPG-21T clustered with Tropicimonas isoalkanivorans B51T (with a sequence similarity of 97.1 %); the novel strain showed lower 16S rRNA gene sequence similarities (<95.4 %) with the other species included in the tree. The mean DNA–DNA relatedness value between strain DPG-21T and T. isoalkanivorans DSM 19548T was 12 %. The predominant ubiquinones of strain DPG-21T were Q-10 and Q-9 while C18 : 1ω7c was the strain’s major fatty acid. The polar lipid profile of strain DPG-21T was similar to that of T. isoalkanivorans DSM 19548T. The genomic DNA G+C content of the novel strain was 69.6 mol%. Some phenotypic properties and the phylogenetic and genetic data indicated that strain DPG-21T was distinct from T. isoalkanivorans and represents a novel species of the genus Tropicimonas, for which the name Tropicimonas aquimaris sp. nov. is proposed. The type strain is DPG-21T ( = KCTC 23424T = CCUG 60524T).

A Gram-negative, facultatively anaerobic, short-rod-shaped, non-motile and non-spore-forming bacterial strain, designated ABP-4T, was isolated from a freshwater spring in Taiwan and was characterized using the polyphasic taxonomy approach. Growth occurred at 20–40 °C (optimum, 30–37 °C), at pH 7.0–10.0 (optimum, pH 7.0–9.0) and with 0–3 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ABP-4T, together with Aquabacterium fontiphilum CS-6T (96.4 % sequence similarity), Aquabacterium commune B8T (96.1 %), Aquabacterium citratiphilum B4T (95.5 %) and Aquabacterium parvum B6T (94.7 %), formed a deep line within the order Burkholderiales. Strain ABP-4T contained summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c), C18 : 1 ω7c and C16 : 0 as predominant fatty acids. The major cellular hydroxy fatty acid was C10 : 0 3-OH. The major isoprenoid quinone was Q-8 and the DNA G+C content was 68.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and several uncharacterized phospholipids. The DNA–DNA relatedness of strain ABP-4T with respect to recognized species of the genus Aquabacterium was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain ABP-4T represents a novel species in the genus Aquabacterium, for which the name Aquabacterium limnoticum sp. nov. is proposed. The type strain is ABP-4T ( = BCRC 80167T = KCTC 23306T).

A novel alphaproteobacterial strain, designated CPCC 100156T, was isolated from a forest soil sample collected from Hainan Island, South China, and subjected to taxonomic investigation using a polyphasic approach. The pink- to rosy-coloured colonies on TSA and YM agar were smooth and moist. Good growth occurred at 28–32 °C and at pH 7.0–7.5. The respiratory quinone was ubiquinone-9. The polar lipids consisted of phosphatidylcholine (PC), hydroxyphosphatidylethanolamine (OH-PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and two unidentified aminolipids (AL1, AL2), with a minor amount of ninhydrin-positive phosphoglycolipid. (NPG). The major cellular fatty acids were summed feature 8 (C18 : 1ω7c /C18 : 1ω6c) (49.5 %), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c) (22.5 %), and C16 : 0 (14.0 %). The G+C content of the genomic DNA was 70.3 mol%. The organism showed 16S rRNA gene sequence similarity of 97.37 % with Belnapia moabensis DSM 16746T. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolate belonged to the family Acetobacteraceae and consistently formed a robust cluster with Belnapia moabensis DSM 16746T in the phylogenetic tree. The DNA–DNA hybridization value between the new isolate and Belnapia moabensis DSM 16746T was 45.6 %. On the basis of the taxonomic evidence, it is proposed that strain CPCC 100156T represents a novel species, for which the name Belnapia rosea sp. nov. is proposed. The type strain is CPCC 100156T ( = DSM 23312T = CGMCC 1.10758T). The description of the genus Belnapia is emended accordingly.

Two psychrophilic strains, Cr7-05T and Cr4-44T, isolated from alpine glacier cryoconite, were characterized by using a polyphasic approach. Both strains were psychrophilic, showing good growth over a temperature range of 1–20 °C. The chemotaxonomic characteristics of these isolates included the presence of C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major cellular fatty acids, Q-10 as the predominant ubiquinone and diphosphatidylglycerol, phosphatidylglycerol and unknown glycolipids as major polar lipids. The DNA G+C contents of strains Cr7-05T and Cr4-44T were 61.4 and 63.6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two isolates belong to the genus Devosia. The 16S rRNA gene sequence similarity between the two strains was 98.6 %, but DNA–DNA hybridization indicated 54 % relatedness. Strains Cr7-05T and Cr4-44T exhibited 16S rRNA gene sequence similarity of 94.7–97.2 and 94.9–96.9 %, respectively, to the type strains of recognized Devosia species. On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, strains Cr7-05T and Cr4-44T represent two novel species within the genus Devosia, for which the names Devosia psychrophila sp. nov. (type strain Cr7-05T  = DSM 22950T  = CGMCC 1.10210T  = CIP 110130T) and Devosia glacialis sp. nov. (type strain Cr4-44T  = CGMCC 1.10691T  = LMG 26051T) are proposed. An emended description of the genus Devosia is also provided.

A Gram-staining-negative, non-spore-forming, facultatively aerobic, non-motile, rod-shaped bacterial strain, BR-3T, was isolated from a tidal flat on the western coast of Korea, and subjected to a polyphasic study. Strain BR-3T grew optimally at 25 °C, at pH 6.5–7.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BR-3T fell within the clade comprising species of the genus Mucilaginibacter, joining the type strain of Mucilaginibacter rigui, with which it exhibited highest 16S rRNA gene sequence similarity (98.2 %). 16S rRNA gene sequence similarity values between strain BR-3T and the type strains of the other species of the genus Mucilaginibacter were in the range 93.8–95.9 %. A mean DNA–DNA relatedness value between strain BR-3T and M. rigui KCTC 12534T was 21 %. Strain BR-3T contained MK-7 as the predominant menaquinone and C16 : 1ω7c and/or iso-C15 : 0 2-OH and iso-C15 : 0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine and an unidentified aminophospholipid. The DNA G+C content was 49.8 mol%. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain BR-3T demonstrated that this strain is separate from M. rigui as well as the other species of the genus Mucilaginibacter. On the basis of the data presented, strain BR-3T is considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter lutimaris sp. nov. is proposed. The type strain is BR-3T ( = KCTC 23461T  = CCUG 60742T).

A Gram-staining-negative, non-flagellated, non-gliding and rod-shaped bacterial strain, designated HWR-17T, was isolated from seawater of the Yellow Sea in Korea. Strain HWR-17T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HWR-17T clustered with the two Mariniflexile species in the family Flavobacteriaceae, exhibiting 16S rRNA gene sequence similarity of 97.1–97.2 % to their type strains and less than 95.7 % sequence similarity to other members of the family Flavobacteriaceae. Strain HWR-17T contained MK-6 as the predominant menaquinone and iso-C15 : 0 as the major fatty acid. The polar lipid profile of strain HWR-17T contained phosphatidylethanolamine, an unidentified aminolipid and four unidentified lipids. The DNA G+C content of strain HWR-17T was 35.7 mol% and it exhibited 11 and 10 % DNA–DNA relatedness, respectively, with Mariniflexile gromovii KCTC 12570T and Mariniflexile fucanivorans DSM 18792T. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain HWR-17T is distinguishable from the two recognized Mariniflexile species. On the basis of the data presented, strain HWR-17T is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile aquimaris sp. nov. is proposed. The type strain is HWR-17T ( = KCTC 23346T  = CCUG 60529T). An emended description of the genus Mariniflexile is also proposed.

The taxonomic position of two strains of a novel heterotrophic, strictly aerobic, Gram-negative, gliding, dark-orange-pigmented bacterium, designated KMM 6217T and KMM 6332, was determined. The strains grew at 10–40 °C and with 0.5–8.0 % NaCl. They were catalase- and oxidase-positive, produced flexirubin-type pigments and hydrolysed aesculin, gelatin, starch, Tween 80 and DNA. The predominant fatty acids were iso-C15 : 1, iso-C15 : 0, C15 : 0, summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c) and iso-C17 : 0 3-OH. The polar lipid profile comprised phosphatidylethanolamine as the major component, three unknown aminolipids and one unknown lipid. The DNA G+C content was 34.7–34.8 mol%. Phylogenetic analysis of the 16S rRNA gene sequence showed that the novel isolates could be placed in the family Flavobacteriaceae. The nearest neighbours of strains KMM 6217T and KMM 6332 were members of the genera Bizionia, Formosa, Lacinutrix, Meridianimaribacter, Mesoflavibacter and Winogradskyella, with sequence similarities to the type strains of species of these genera of 92.8–95.2, 93.7–94.5, 94.1–94.7, 94.9, 94.6 and 93.5–94.4 %, respectively. Based on phenotypic, genotypic and phylogenetic data, strains KMM 6217T and KMM 6332 should be classified as representatives of a novel species in a new genus in the family Flavobacteriaceae of the phylum ‘Bacteroidetes’, for which the name Corallibacter vietnamensis gen. nov., sp. nov. is proposed; the type strain is KMM 6217T ( = JCM 17525T = KCTC 23026T).

A Gram-staining-negative, non-motile, non-spore-forming bacterial strain, DPG-3T, was isolated from seawater from the South Sea in Korea, and its taxonomic position was investigated using a polyphasic approach. Strain DPG-3T grew optimally at 30 °C and in the presence of 2 % (w/v) NaCl. In a neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain DPG-3T fell within a clade comprising Algoriphagus species and appeared most closely related to Algoriphagus halophilus JC 2051T (96.1 %16S rRNA gene sequence similarity) and Algoriphagus lutimaris S1-3T (96.4 %). The type strains of other Algoriphagus species showed 16S rRNA gene sequence similarities of 92.9–96.0 % with strain DPG-3T. The predominant menaquinone of strain DPG-3T was MK-7. The major fatty acids were iso-C15 : 0 and iso-C15 : 0 2-OH and/or C16 : 1ω7c (summed feature 3). The major polar lipids detected in strain DPG-3T were phosphatidylcholine, phosphatidylethanolamine and an unidentified lipid. The genomic DNA G+C content was 44.8 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain DPG-3T is considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus namhaensis sp. nov. is proposed. The type strain is DPG-3T ( = KCTC 23419T = CCUG 60523T).

Three exopolysaccharide-producing bacteria, designated strains DRP28T, DRP29 and DRP31, were isolated from the rhizoplane of Angelica sinensis from the Geumsan, Republic of Korea. Cells were straight rods, Gram reaction-negative, aerobic, non-motile, and catalase- and oxidase- positive. Flexirubin-type pigments were absent. Phylogenetic analysis of the 16S rRNA gene indicated that these bacteria belong to the genus Mucilaginibacter in the phylum Bacteroidetes. 16S rRNA gene sequence similarities to strains of recognized species of the genus Mucilaginibacter were 93.8–97.4 %. The major fatty acids were iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The strains contained MK-7 as the major isoprenoid quinone. Strains DRP28T, DRP29 and DRP31 formed a single, distinct genomospecies with DNA G+C contents of 41.9–42.7 mol% and DNA hybridization values of 82.6–86.8 %; the strains exhibited DNA–DNA hybridization values of only 20.4–41.3 % with related species of the genus Mucilaginibacter. On the basis of evidence presented in this study, strains DRP28T, DRP29 and DRP31 were considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter polysacchareus sp. nov. is proposed. The type strain is DRP28T ( = KACC 15075T  = NBRC 107757T).

Two bacterial isolates from the surface water of a haloalkaline lake on the Mongolia Plateau, strains MIM18T and MIM19, were characterized; their morphological, physiological and chemotaxonomic characters, and phylogenetic position based on 16S rRNA gene sequences, were determined. The two strains were alkaliphilic, halotolerant, non-motile, aerobic, Gram-negative, orange-red, rod-shaped and oxidase-positive. Growth was observed in 0–5.5 % (w/v) NaCl, with optimum growth at 1 %. The temperature range for growth was 0–41 °C, with good growth at 28–37 °C and optimum growth at 30–33 °C. The DNA G+C content was 39.8–41.2 mol%. The strains contained menaquinone MK-7 as the major respiratory quinone and phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. Predominant cellular fatty acids were iso-C15 : 0 (28.1–29.3 %), iso-C17 : 0 3-OH (14.6–18.8 %), C15 : 1ω6c (5.3–8.6 %), C14 : 0 2-OH and/or iso-C15 : 0 2-OH (5.4–6.1 %), and iso-C17 : 1ω9c and/or C16 : 0 10-methyl (5.0–6.8 %). 16S rRNA gene sequence analysis showed that Belliella and Nitritalea of the family Cyclobacteriaceae were the closest related species with sequence similarities of 91.7–92.3 % and 88.2 %, respectively, with strains of these genera; other members of the Cyclobacteriaceae had sequence similarities lower than 88 %. Phylogenetic analysis indicated that the strains formed a deep-rooted lineage distinct from the clades represented by the genera Belliella, Nitritalea, Indibacter, Aquiflexum, Echinicola, Litoribacter, Cyclobacterium and Algoriphagus. Based on the phenotypic and phylogenetic characteristics mentioned above, the two strains are representatives of a single novel species in a new genus; the name Mongoliitalea lutea gen. nov., sp. nov. is proposed, with MIM18T ( = ACCC 05421T = KCTC 23224T) as the type strain.

A Gram-negative-staining, rod-shaped, non-motile and pink bacterial strain was isolated from the soil of a Populus euphratica forest located in Xinjiang, China. The strain, designated strain HYL7-15T, was subjected to a taxonomic analysis using a polyphasic approach. 16S rRNA gene sequence analyses indicated that the isolate belonged to the phylum Bacteroidetes and was related to the genus Pontibacter, with sequence similarities ranging from 93.1 to 95.0 % with other species of the genus Pontibacter. Strain HYL7-15T contained MK-7 as the predominant menaquinone and its DNA G+C content was 44.9 mol%. The major cellular fatty acids of the novel strain were iso-C15 : 0 (16.49 %), iso-C17 : 0 3-OH (10.96 %) and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I, 18.46 %). The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG); diphosphatidylglycerol (DPG), three unknown aminophospholipids (APLs) and two unknown phospholipids (PLs) were also detected. On the basis of the evidence presented, it is concluded that strain HYL7-15T represents a novel species of the genus Pontibacter, for which the name Pontibacter populi sp. nov. is proposed. The type strain is HYL7-15T ( = CCTCC AB 206239T = NRRL B-59488T).

A Gram-staining-negative, aerobic to microaerophilic, rod-shaped, red-coloured bacterium, strain T4T, was isolated from a freshwater pipe on Tenerife island. A polyphasic taxonomic study was performed in order to characterize the strain in detail. The isolate is surrounded by a slime capsule, occurs singly, in the form of short chains, or in aggregates, and exhibits catalase and oxidase activities. Growth was observed at 15–42 °C. Optimum growth occurred at pH 8 with mono- and disaccharides, followed by polysaccharides and deoxysaccharides, but the bacterium utilized only a restricted spectrum of alcohols, alditols, amides, amines, carboxylic acids and amino acids. Strain T4T tolerated concentrations of 0–4 % (w/v) NaCl and contained MK-7 as predominant isoprenoid quinone as well as carotenoids, but lacked pigments of the flexirubin type. The predominant fatty acids were iso-C15 : 0 (32.2 %), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c; 22.5 %), and iso-C17 : 0 3-OH (7.9 %). Major polar lipids were phosphatidylethanolamine, phospholipids, aminophospholipids and other lipids of unknown character. The DNA G+C content was approximately 41.8 mol%. The sequence of the 16S-rRNA gene assigned strain T4T to the CFB group, forming a coherent cluster with species of the genus Algoriphagus with the highest similarity of 98.8 % to Algoriphagus aquatilis A8-7T. DNA–DNA hybridization revealed 37.5 % relatedness to strain A8-7T. Based on morphological, physiological and molecular properties as well as on phylogenetic distinctiveness, strain T4T should be placed into the genus Algoriphagus as a novel species, for which the name Algoriphagus aquaeductus sp. nov. (type strain T4T  = DSM 19759T  = LMG 24398T  = NCIMB 14399T) is proposed.

A Gram-negative, non-motile, non-spore-forming, non-flagellated rod capable of degrading cypermethrin, designated LQY-18T, was isolated from activated sludge of a wastewater treatment plant in China. Strain LQY-18T grew at 8–40 °C (optimum 30 °C), at pH 5.0–10.0 (optimum pH 7.0) and with 0–5 % (w/v) NaCl (optimum 1 %). The predominant menaquinone was MK-7 (97 %) and the major fatty acids were summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c), iso-C15 : 0 and iso-C17 : 0 3-OH. The DNA G+C content was 40.3 mol%. Phylogenetic analysis revealed that the isolate belonged to the genus Sphingobacterium of the phylum Bacteroidetes and showed low 16S rRNA gene sequence similarity with recognized members of the genus Sphingobacterium. The closest neighbour was Sphingobacterium mizutaii ATCC 33299T (92.9 % 16S rRNA gene sequence similarity). On the basis of phenotypic, genetic and phylogenetic data, strain LQY-18T ( = ACCC 05410T = CCTCC AB 2010005T = KCTC 23009T) should be classified as a representative of a novel species of the genus Sphingobacterium, for which the name Sphingobacterium wenxiniae sp. nov. is proposed.

An aerobic, pink-pigmented, chemo-organotrophic bacterium, designated strain SN10T, was isolated from a methanotrophic enrichment culture obtained from an acidic Sphagnum peat. This isolate was represented by Gram-negative, non-motile rods that multiply by normal cell division and form rosettes. Strain SN10T is an obligately acidophilic, mesophilic bacterium capable of growth at pH 3.2–6.6 (with an optimum at pH 4.7–5.2) and at 6–32 °C (with an optimum at 20–24 °C). The preferred growth substrates are sugars and several heteropolysaccharides of plant and microbial origin, such as pectin, lichenan, fucoidan and gellan gum. While not being capable of growth on C1 compounds, strain SN10T can develop in co-culture with exopolysaccharide-producing methanotrophs by utilization of their capsular material. The major fatty acids determined in strain SN10T using the conventional lipid extraction procedure are iso-C15 : 0 and C16 : 1ω7c. Upon hydrolysis of total cell material, substantial amounts of the uncommon membrane-spanning lipid 13,16-dimethyl octacosanedioic acid (isodiabolic acid) were also detected. The polar lipids are two phosphohexoses, phosphatidylethanolamine, phosphatidylglycerol and several phospholipids of unknown structure. The major quinone is MK-8. Pigments are carotenoids. The G+C content of the DNA is 60.7 mol%. Strain SN10T forms a separate lineage within subdivision 1 of the phylum Acidobacteria and displays 94.0–95.4 % 16S rRNA gene sequence similarity to members of the genera Edaphobacter and Granulicella, 93.0–93.7 % similarity to members of the genus Terriglobus and 92.2–92.3 % similarity to the type strains of Telmatobacter bradus and Acidobacterium capsulatum. Therefore, strain SN10T is classified within a novel genus and species, for which the name Bryocella elongata gen. nov., sp. nov. is proposed. Strain SN10T ( = LMG 25276T  = DSM 22489T) is the type strain of Bryocella elongata. An emended description of Edaphobacter aggregans Koch et al. 2008 is also given.

During an investigation of yeast biota in the rhizosphere of lentisk in Sardinian semi-arid areas, a strain was isolated that could not be assigned to any known species. The sequence of the D1/D2 domain of the large subunit rDNA gene revealed that the strain belonged to the genus Kazachstania and was phylogenetically related to a clade including Kazachstania aerobia, Kazachstania servazzii, Kazachstania solicola and Kazachstania unispora. The novel isolate differed from members of this clade in its ability to assimilate d-glucono-1,5-lactone and its very weak fermentation of glucose and sucrose; its assimilation profile was unique within the genus Kazachstania. Monosporal colonies were able to sporulate, indicating that the species is homothallic. It is proposed that the isolate represents a novel species, Kazachstania ichnusensis sp. nov., with LCF 1675T ( = CBS 11859T) as type strain.