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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 76, Issue 4

sp. nov., isolated from rhizosphere soil of and proposal of Higashide . 1966 (Approved Lists 1980) as a later heterotypic synonym of Owen . 1963 (Approved Lists 1980) No Access

Abstract

A novel actinobacterium strain BI20, isolated from the rhizosphere of , was subjected to a polyphasic taxonomic study. Analysis of the 16S rRNA gene sequence showed that strain BI20 belonged to the genus and shared the highest sequence similarities to Streptomyces cinnamoneus JCM 4633 (98.75%), JCM 4586 (98.74%) and NBRC 12518 (98.68%). Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences indicated that strain BI20 was most related to ATCC 31255. However, the average nucleotide identity by MUMmer (ANIm) and digital DNA–DNA hybridization (dDDH) values between strain BI20 and the above four strains were 84.88–85.75% and 22.2–23.2%, respectively, well below 96.7% and 70% cut-off points recommended for delineating species. Whole-cell hydrolysates of strain BI20 contained diaminopimelic acid and whole-cell sugars contained glucose. The predominant cellular fatty acids (>10%) were C, C, C and C. All these data indicated that strain BI20 represents a novel species, for which the name sp. nov. is proposed. The type strain is strain BI20 (=MCCC 1K09588=JCM 38134). In addition, we propose that Higashide . 1966 (Approved Lists 1980) is a later heterotypic synonym of Owen . 1963 (Approved Lists 1980) based on comparative genomic analysis. Meanwhile, based on the dDDH value, and are simultaneously designated as two different subspecies, i.e. subsp. and subsp. .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinobacteria , polyphasic taxonomy , rhizosphere soil , Streptomyces and taxonomic analysis
Funding
This study was supported by the:
  • Natural Science Foundation of Hunan Province (Award 2022JJ50125)
    • Principal Award Recipient: RongjianGao
  • Natural Science Foundation of Hunan Province (Award 2022JJ50125)
    • Principal Award Recipient: YaziChen
  • Natural Science Foundation of Hunan Province (Award 2022JJ50125)
    • Principal Award Recipient: YanXiao
  • Natural Science Foundation of Hunan Province (Award 2022JJ50125)
    • Principal Award Recipient: JianGao
© 2026 The Authors
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2026-04-02
2026-04-22

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Streptomyces dryopteridis sp. nov., isolated from rhizosphere soil of Dryopteris marginalis and proposal of Streptomyces cuspidosporus Higashide et al. 1966 (Approved Lists 1980) as a later heterotypic synonym of Streptomyces sparsogenes Owen et al. 1963 (Approved Lists 1980)
Int J Syst Evol Microbiol 76, 007104 (2026); https://doi.org/10.1099/ijsem.0.007104
/content/journal/ijsem/10.1099/ijsem.0.007104
/content/journal/ijsem/10.1099/ijsem.0.007104
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