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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 76, Issue 3

sp. nov., a novel carotenoid-producing actinobacterium isolated from saline lake sediment No Access

Abstract

An actinobacterial strain, designated TRA05-7, was isolated from a saline lake sediment in the Ngari Prefecture, Xizang Autonomous Region, China. The cells were Gram-stain-positive, aerobic and short-rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRA05-7 was most closely related to members of the genus , with the highest sequence similarity to 194 (98.48%). However, whole-genome phylogenetic analysis revealed a closer relationship with SYSU T00001, with the two strains forming a distinct cluster. The average nucleotide identity and digital DNA–DNA hybridization values between strain TRA05-7 and were 77.3 and 20.7%, respectively. The genomic DNA G+C content was 65.7 mol%. Growth occurred at 4–40 °C (optimum 28 °C), pH 7–11 (optimum pH 7) and with 0–8% (w/v) NaCl (optimum 1%). The major menaquinones were MK-12, MK-11 and MK-13. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid. The major cellular fatty acids were anteiso-C, anteiso-C and anteiso-C. UV-Vis and HPLC analysis indicated that strain TRA05-7 had the ability to produce carotenoids. Based on phylogenetic, genomic, phenotypic and chemotaxonomic analyses, strain TRA05-7 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TRA05-7 (=MCCC 1K10024=KCTC 59574).

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  • Accepted:
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Keyword(s): genome , Homoserinimonas flava , polyphasic taxonomy and saline lake
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32360004)
    • Principal Award Recipient: LiTuo
  • Guizhou Provincial Science and Technology Foundation (Award Qian Ke He Jichu-ZK [2024] Yiban 270)
    • Principal Award Recipient: LiTuo
  • Natural Science Foundation of Hunan Province, China (Award 2024JJ8015)
    • Principal Award Recipient: FangLiu
  • Zunyi City Science and Technology project (Award Zunyi City, Kehe HZ character (2025) No.269)
    • Principal Award Recipient: LiTuo
© 2026 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.007085
2026-03-05
2026-04-19

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Homoserinimonas flava sp. nov., a novel carotenoid-producing actinobacterium isolated from saline lake sediment
Int J Syst Evol Microbiol 76, 007085 (2026); https://doi.org/10.1099/ijsem.0.007085
/content/journal/ijsem/10.1099/ijsem.0.007085
/content/journal/ijsem/10.1099/ijsem.0.007085
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