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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 11

sp. nov. and sp. nov., isolated from marine sponges No Access

Abstract

Two Gram-stain-negative, facultatively anaerobic, non-motile and rod-shaped bacterial strains, designated 2201CG32-9 and 2304DJ12-6, were isolated from marine sponges collected in the Republic of Korea. Both strains exhibited catalase and oxidase activities but tested negative for nitrate reduction and indole production. Strain 2201CG32-9 showed optimal growth at 30 °C and pH 7.0–8.0 in the presence of 3% (w/v) NaCl, whereas strain 2304DJ12-6 grew optimally at 25 °C, pH 8.0 and 3% (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene and phylogenomic analysis of genome sequences indicated that strains 2201CG32-9 and 2304DJ12-6 represented novel species within the genus . Based on whole-genome phylogenomic analysis, the two strains were most closely related to OS29 and ATCC 700307, respectively. The average nucleotide identity values of these strains were found to be <75.9%, and their digital DNA–DNA hybridization values were <22.5%. The DNA G+C contents of strains 2201CG32-9 and 2304DJ12-6 were 56.5 and 53.5 mol%, respectively. In both strains, ubiquinone-8 was identified as the predominant respiratory quinone, and the major cellular fatty acids (>5.0%) were C, iso-C 3-OH, iso-C, iso-C, summed feature 8 and summed feature 9. As for polar lipids, both strains comprised phosphatidylglycerol, phosphatidylethanolamine and phosphoglycolipid as common major polar lipids. Strains 2201CG32-9 and 2304DJ12-6 are classified as two novel species of the genus based on a polyphasic taxonomic approach, for which the names sp. nov. (2201CG32-9=KACC 23829=JCM 37223) and sp. nov. (2304DJ12-6=KACC 23832=MCCC 1K09533) are proposed, respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): marine sponge , Microbulbifer discodermiae , Microbulbifer jejuensis and new taxa
Funding
This study was supported by the:
  • National Institute for International Education (Award 2021R1I1A3046479)
    • Principal Award Recipient: Jin-SookPark
  • National Marine Biodiversity Institute of Korea
    • Principal Award Recipient: Jin-SookPark
© 2025 The Authors
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2025-11-27
2025-12-15

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Microbulbifer discodermiae sp. nov. and Microbulbifer jejuensis sp. nov., isolated from marine sponges
Int J Syst Evol Microbiol 75, 006980 (2025); https://doi.org/10.1099/ijsem.0.006980
/content/journal/ijsem/10.1099/ijsem.0.006980
/content/journal/ijsem/10.1099/ijsem.0.006980
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