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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 11

gen. nov., sp. nov., isolated from a biofilm of a membrane bioreactor (MBR) treating sewage Open Access

Abstract

Novel Gram-stain-negative, non-spore-forming, non-motile rods, designated HTMS2 and HTMS3, were isolated from a biofilm on the membrane of a municipal sewage treatment membrane bioreactor in Nagaoka, Japan. Phylogenetic analysis of 16S rRNA genes placed them in the family , most closely related to A2P5 (94.14% similarity). Genome sequencing (2.52 Mb, 48.2% G+C) and phylogenomic analysis affiliated them with the uncultured genus lineage CTSOIL-112 in the Genome Taxonomy Database. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values to related taxa were below species thresholds (ANI ≤77.0%, dDDH ≤54.9%). In addition, average amino acid identity values to related taxa were ≤68.6%. Both strains are catalase- and oxidase-positive, reduced nitrate and utilized various carbohydrates but not -acetyl-glucosamine; they differed in sorbitol assimilation. The major respiratory quinone is Q-8, and the predominant fatty acids are summed feature 3 (C_16:1 ω7c/ω6c), summed feature 8 (C_18:1 ω7c/ω6c) and C_16:0. Genomic data supported a non-motile, Gram-stain-negative phenotype. Polyphasic analysis indicates that these strains represent a novel genus and species, gen. nov., sp. nov. (type strain HTMS3=NBRC 117344=LMG 34133).

  • Received:
  • Accepted:
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Keyword(s): activated sludge , biofilm , Burkholderiaceae and g_CTSOIL-112
Funding
This study was supported by the:
  • JST FOREST Program (Award JPMJFR225T)
    • Principal Award Recipient: MasashiHatamoto
  • Japan Society for the Promotion of Science (Award JP24H00333)
    • Principal Award Recipient: MasashiHatamoto
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2025-11-20
2025-12-15

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Puribacter membranae gen. nov., sp. nov., isolated from a biofilm of a membrane bioreactor (MBR) treating sewage
Int J Syst Evol Microbiol 75, 006978 (2025); https://doi.org/10.1099/ijsem.0.006978
/content/journal/ijsem/10.1099/ijsem.0.006978
/content/journal/ijsem/10.1099/ijsem.0.006978
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