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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 11

sp. nov., isolated from mangrove sediment No Access

Abstract

A Gram-stain-negative, strictly aerobic and motile bacterium, designated as FT89, was isolated from mangrove sediment in Shenzhen, China. Phylogenetic analysis of strain FT89 was mostly related to , with 16S rRNA gene similarity of 99.66%. The genome size of strain FT89 was 5,065,293 bp and the calculated G+C molar content was 65.90mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) value towards yielded 93.86% ANI and 53.2% dDDH. Although the 16S rRNA gene similarity is above 98.7%, the ANI and dDDH values are below the established species delineation thresholds (ANI <95% and dDDH <70%). Here, we confirm that strain FT89 represents a novel species. Phenotypic characterization revealed that cells of strain FT89 were ovoid to rod shaped, measuring 0.3–0.7 µm in width and 0.9–3.2 µm in length. Strain FT89 grew at 20–40 °C (optimum 35 °C) at pH 5.5–9.0 (optimum pH 6.0–7.0) with 0–9% (w/v) NaCl (optimum 1–2%). The cells of strain FT89 contained Q-10, and the major cellular fatty acids were composed of C cyclo 8 (41.7%) and summed feature 8 (C 7 and/or C 6) (32.4%). Based on phylogenetic, phenotypic and chemotaxonomic analysis, strain FT89 should represent a novel species of the genus , for which the proposed name is FT89 (=MCCC 1K08485=KCTC 8079).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): mangrove sediment , Microbaculum mangrovi and Tepidamorphaceae
Funding
This study was supported by the:
  • Basic and Applied Basic Research Foundation of Guangdong Province (Award 2025A1515010475)
    • Principal Award Recipient: XinxuZhang
  • Shenzhen Science and Technology Program (Award JCYJ20241202124403006)
    • Principal Award Recipient: XinxuZhang
  • Shenzhen University 2035 Program for Excellent Research (Award 2022B002)
    • Principal Award Recipient: MengLI
  • Shenzhen Science and Technology Program (Award KCXFZ20240903092800002)
    • Principal Award Recipient: MengLI
  • Guangdong Major Project of Basic and Applied Basic Research (Award 2023B0303000017)
    • Principal Award Recipient: MengLI
© 2025 The Authors
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2025-11-19
2025-12-08

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Microbaculum mangrovi sp. nov., isolated from mangrove sediment
Int J Syst Evol Microbiol 75, 006972 (2025); https://doi.org/10.1099/ijsem.0.006972
/content/journal/ijsem/10.1099/ijsem.0.006972
/content/journal/ijsem/10.1099/ijsem.0.006972
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