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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 11

sp. nov., isolated from the honeybee No Access

Abstract

The genus currently includes 12 species with validly published names, the majority of which are closely associated with honeybees. A Gram-positive, facultatively anaerobic, catalase-negative and fructose-dependent strain, designated 1-1-2, was isolated from the gut of a honeybee (). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1-1-2ᵀ represents a distinct lineage within the genus , with its closest phylogenetic neighbours being YH-15 (99.8% sequence similarity), HN36-1 (99.8%) and Fhon13N (98.8%). The complete genome of strain 1-1-2 comprised 1,596,221 bp, with a G+C content of 37.1 mol%. Pairwise comparisons of average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain 1-1-2 and its closest phylogenetic neighbours – DSMZ 12361, HN36-1 and Fhon13N – revealed ANI values ranging from 80.1% to 93.8% and dDDH values ranging from 22.6% to 52.1%. Strain 1-1-2 can also be distinguished from its closest relatives based on phenotypic and chemotaxonomic characteristics. Accordingly, we propose that strain 1-1-2 represents a novel species within the genus , for which the name sp. nov. is proposed, with 1-1-2 (=BCRC 81468=NBRC 117233) as the type strain.

  • Received:
  • Accepted:
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Keyword(s): Apilactobacillus sp. nov. , honeybee , lactic acid bacteria (LAB) and Taiwan
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2025-12-15

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Apilactobacillus intestinapis sp. nov., isolated from the honeybee
Int J Syst Evol Microbiol 75, 006971 (2025); https://doi.org/10.1099/ijsem.0.006971
/content/journal/ijsem/10.1099/ijsem.0.006971
/content/journal/ijsem/10.1099/ijsem.0.006971
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