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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 11

sp. nov., isolated from leaves of coltsfoot growing in mercury-contaminated soil No Access

Abstract

Three mercury-resistant isolates of a novel species were obtained from the leaves of coltsfoot ( L.) growing in mercury-contaminated soil in Southern Poland. Using the 16S rRNA gene sequence, they were identified as likely isolates. Whole-genome analyses based on phylogenomic analyses and overall genome relatedness indices showed that they are three nearly identical isolates of a novel species, with KL28 as the closest type species by overall genome relatedness indices [86.64% average nucleotide identity (ANI), 34.2% digital DNA–DNA hybridization (dDDH)] and with P66 (83.85% ANI, 28.5% dDDH) and 119P (83.66% average nucleotide identity, 28.4% dDDH) as the two closest by maximum likelihood phylogenomic analysis. Moreover, five genomes publicly available in the databases were found. One of the three isolates, Hg5Tf, was further characterized as Gram-negative, motile, facultatively aerobic rods, which can be distinguished from closely related species by the (delayed) positive urease reaction in the API20NE test and by weak growth on pectin, -lactic acid methyl ester and acetoacetic acid in the BIOLOG Gen III test. Matrix-assisted laser-desorption/ionization time-of-flight profile of Hg5Tf is also distinct from those of closely related species. A novel species of genus is proposed with the name sp. nov. and Hg5Tf (=CCM 9461=PCM 3563) as its type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bacterial plant symbiont , genome analysis and phylogenomic analysis
Funding
This study was supported by the:
  • Uniwersytet Jagielloński w Krakowie (Award 19000882)
    • Principal Award Recipient: DariuszLatowski
© 2025 The Authors
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2025-11-14
2025-12-16

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Pseudomonas tussilaginis sp. nov., isolated from leaves of coltsfoot growing in mercury-contaminated soil
Int J Syst Evol Microbiol 75, 006968 (2025); https://doi.org/10.1099/ijsem.0.006968
/content/journal/ijsem/10.1099/ijsem.0.006968
/content/journal/ijsem/10.1099/ijsem.0.006968
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