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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 11

sp. nov., a novel ascomycetous yeast species isolated from rotting wood and soil in Japan No Access

Abstract

Three yeast strains closely related to the genus were isolated from rotting wood () and soil in Japan. Based on sequence analyses of the internal transcribed spacer (ITS1–5.8S–ITS2) region, the D1/D2 domain of the LSU rRNA gene and the mitochondrial gene, together with physiological characterization, these isolates were determined to represent a novel species. The ITS and D1/D2 sequences of the three strains were nearly identical, differing only by a single nucleotide, and their physiological profiles were indistinguishable, supporting their conspecificity. Notably, the strains actively fermented -glucose and -fructose. They exhibited tolerance to high osmotic stress, being capable of growth in media containing either 10% sodium chloride with 5% glucose or up to 50% (w/v) glucose. Furthermore, under V8 agar culture conditions, they consistently produced four ascospores per ascus. Phylogenetic analyses based on ITS and LSU D1/D2 sequences clearly placed these strains within the genus but distinct from other known species. In addition, comparison of gene sequences further supported their separation from related species. Based on these phylogenetic and phenotypic characteristics, we propose sp. nov. to accommodate these isolates. The holotype of sp. nov. is designated as NBRC 117259.

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Keyword(s): Saccharomycetaceae , Vanderwaltozyma and yeast
© 2025 The Authors
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2025-11-07
2025-12-16

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Vanderwaltozyma osmotolerans sp. nov., a novel ascomycetous yeast species isolated from rotting wood and soil in Japan
Int J Syst Evol Microbiol 75, 006965 (2025); https://doi.org/10.1099/ijsem.0.006965
/content/journal/ijsem/10.1099/ijsem.0.006965
/content/journal/ijsem/10.1099/ijsem.0.006965
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