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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 11

sp. nov., isolated from Atlantic salmon ( L.) fish farmed in Chile Open Access

Abstract

Strain P3-BQ1 is a Gram-negative, aerobic, non-spore-forming bacterium with gliding motility and filamentous cells. It was isolated in 2018 from the gills of a diseased Atlantic salmon () during an outbreak of tenacibaculosis in a Chilean fish farm. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain P3-BQ1 belongs to the genus and is most closely related to 35/09 (98.53%), ‘’ 18-2881-A (98.46%), JDTF-79 (98.46%), TNO010 (97.85%) and TNO20 (97.41%). The genome size of strain P3-BQ1 is 2,777,603 bp, with a DNA G+C content of 29.18 mol%. The strain has an OrthoANI score lower than 95% compared to the types of all validly named species, with the closest neighbour being 35/09 (score 87.41 %). In contrast, P3-BQ1ᵀ and ‘’ 18-2881-A shared a 96.58%, thus exceeding the species threshold. This result is also supported by whole-genome DNA–DNA hybridization/Genome-to-Genome Distance Calculator values, indicating that strains P3-BQ1 and 18-2881-A are conspecific. Strain P3-BQ1 contains MK-6 as its sole detectable menaquinone. The polar lipids profile includes glycolipids (=2), aminolipids (=3) and unidentified lipids (=5). The predominant cellular fatty acids (>5 %) are C, C, iso-C, iso-C 3-OH and summed feature 3 (Cω7с/Cω6с). An immersion challenge was conducted to assess the pathogenic potential in Atlantic salmon. Our findings indicate that strain P3-BQ1 alone does not cause significant mortality, suggesting it is likely non-pathogenic to this species. Based on the phenotypic, phylogenetic and genotypic data presented, sp. nov. is proposed here with type strain P3-BQ1 (=IMI 507635=RGM 3581), being 18-2881-A an additional strain.

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Keyword(s): Atlantic salmon , Chile , fish aquaculture and tenacibaculosis
Funding
This study was supported by the:
  • Agencia Nacional de Investigación y Desarrollo (Award FONDAP 1523A0007)
    • Principal Award Recipient: RubenAvendaño-Herrera
  • Agencia Nacional de Investigación y Desarrollo (Award FONDECYT 1230068)
    • Principal Award Recipient: RubenAvendaño-Herrera
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2025-11-14
2025-12-15

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References

  1. Suzuki M. Genus Tenacibaculum. In Bergey´s Manual of Systematics of Archae and Bacteria, Online John Wiley & Sons, Inc. in association with Bergey´s Manual Trust; 2015
     [Google Scholar]
  2. Avendaño-Herrera R, Toranzo AE, Magariños B. Tenacibaculosis infection in marine fish caused by Tenacibaculum maritimum: a review. Dis Aquat Organ 2006; 71:255–266 [View Article] [PubMed]
     [Google Scholar]
  3. Olsen AB, Powell J, Siah A, Colquhoun DJ, Avendaño-Herrera R. Tenacibaculosis. In Brun E, Rodríguez JF, Macdonald A. eds Technical Report: An Overview of Emerging Diseases in Salmonid Farming Industry Guelph: Aqua Global technical Services Elanco Canada Ltd., Research Park Center; 2019 pp 43–51
     [Google Scholar]
  4. Mabrok M, Algammal AM, Sivaramasamy E, Hetta HF, Atwah B et al. Tenacibaculosis caused by Tenacibaculum maritimum: updated knowledge of this marine bacterial fish pathogen. Front Cell Infect Microbiol 2023; 12:1068000 [View Article]
     [Google Scholar]
  5. SERNAPESCA Informe con antecedentes sanitarios de agua dulce y mar, 1o 432 semestre 2023. n.d https://www.sernapesca.cl/app/uploads/2023/12/Informe-Sanitario-1S-2023-Publicacion-002.pdf
  6. Avendaño-Herrera R, Irgang R, Sandoval C, Moreno-Lira P, Houel A et al. Isolation, characterization and virulence potential of Tenacibaculum dicentrarchi in salmonid cultures in Chile. Transbound Emerg Dis 2016; 63:121–126 [View Article]
     [Google Scholar]
  7. Apablaza P, Frisch K, Brevik ØJ, Småge SB, Vallestad C et al. Primary isolation and characterization of Tenacibaculum maritimum from Chilean Atlantic Salmon mortalities associated with a Pseudochattonella spp. algal bloom. J Aquat Anim Health 2017; 29:143–149 [View Article] [PubMed]
     [Google Scholar]
  8. Valdes S, Irgang R, Barros MC, Ilardi P, Saldarriaga-Córdoba M et al. First report and characterization of Tenacibaculum maritimum isolates recovered from rainbow trout (Oncorhynchus mykiss) farmed in Chile. J Fish Dis 2021; 44:1481–1490 [View Article] [PubMed]
     [Google Scholar]
  9. Bridel S, Olsen A-B, Nilsen H, Bernardet J-F, Achaz G et al. Comparative genomics of Tenacibaculum dicentrarchi and “Tenacibaculum finnmarkense” highlights intricate evolution of fish-pathogenic species. Genome Biol Evol 2018; 10:452–457 [View Article]
     [Google Scholar]
  10. Avendaño-Herrera R, Saldarriaga-Córdoba M, Irgang R. Draft genome sequence of Tenacibaculum ovolyticum to-7Br, recovered from a farmed Atlantic Salmon (Salmo salar). Microbiol Resour Announc 2022; 11:e0025422 [View Article] [PubMed]
     [Google Scholar]
  11. Avendaño‐Herrera R, Olsen AB, Saldarriaga‐Cordoba M, Colquhoun DJ, Reyes V et al. Isolation, identification, virulence potential and genomic features of Tenacibaculum piscium isolates recovered from Chilean salmonids. Transbound Emerg Dis 2022; 69:e3305–e3315 [View Article]
     [Google Scholar]
  12. Avendaño-Herrera R, Saldarriaga-Córdoba M, Irgang R. Tenacibaculum bernardetii sp. nov., isolated from Atlantic salmon (Salmo salar L.) cultured in Chile. Int J Syst Evol Microbiol 2023; 73: [View Article]
     [Google Scholar]
  13. Avendaño-Herrera R, Collarte C, Saldarriaga-Córdoba M, Irgang R. New salmonid hosts for Tenacibaculum species: expansion of tenacibaculosis in Chilean aquaculture. J Fish Dis 2020; 43:1077–1085 [View Article] [PubMed]
     [Google Scholar]
  14. Nowlan JP, Sies AN, Britney SR, Cameron ADS, Siah A et al. Genomics of Tenacibaculum Species in British Columbia, Canada. Pathogens 2023; 12:101 [View Article]
     [Google Scholar]
  15. Pazos F, Santos Y, Macías AR, Núñez S, Toranzo AE. Evaluation of media for the successful culture of Flexibacter maritimus. J Fish Dis 1996; 19:193–197 [View Article]
     [Google Scholar]
  16. Avendaño-Herrera R, Irgang R, Tapia-Cammas D. PCR procedure for detecting the fish pathogen Tenacibaculum dicentrarchi. J Fish Dis 2018; 41:715–719 [View Article] [PubMed]
     [Google Scholar]
  17. Lane DJ. 16S/23S rRNA sequencing. In Stackbrandt E, Goodfellow M. eds Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp 115–175
     [Google Scholar]
  18. Katoh K, Rozewicki J, Yamada KD. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 2019; 20:1160–1166 [View Article] [PubMed]
     [Google Scholar]
  19. Lemoine F, Correia D, Lefort V, Doppelt-Azeroual O, Mareuil F et al. NGPhylogeny.fr: new generation phylogenetic services for non-specialists. Nucleic Acids Res 2019; 47:W260–W265 [View Article]
     [Google Scholar]
  20. Olson RD, Assaf R, Brettin T, Conrad N, Cucinell C et al. Introducing the Bacterial and Viral Bioinformatics Resource Center (BV-BRC): a resource combining PATRIC, IRD and ViPR. Nucleic Acids Res 2023; 51:D678–D689 [View Article]
     [Google Scholar]
  21. Vallenet D, Calteau A, Dubois M, Amours P, Bazin A et al. MicroScope: an integrated platform for the annotation and exploration of microbial gene functions through genomic, pangenomic and metabolic comparative analysis. Nucleic Acids Res 2019; 48:D579–D589 [View Article]
     [Google Scholar]
  22. Lopez P, Bridel S, Saulnier D, David R, Magariños B et al. Genomic characterization of Tenacibaculum maritimum O‐antigen gene cluster and development of a multiplex PCR‐based serotyping scheme. Transbounding Emerging Dis 2022; 69:e2876–e2888 [View Article]
     [Google Scholar]
  23. Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004; 32:1792–1797 [View Article] [PubMed]
     [Google Scholar]
  24. Lee I, Ouk Kim Y, Park SC, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article] [PubMed]
     [Google Scholar]
  25. Meier-Kolthoff JP, Carbasse JS, Peinado-Olarte RL, Göker M. TYGS and LPSN: a database tandem for fast and reliable genome-based classification and nomenclature of prokaryotes. Nucleic Acids Res 2022; 50:D801–D807 [View Article] [PubMed]
     [Google Scholar]
  26. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 2009; 106:19126–19131 [View Article] [PubMed]
     [Google Scholar]
  27. Moore WEC, Stackebrandt E, Kandler O, Colwell RR, Krichevsky MI et al. Report of the Ad Hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [View Article]
     [Google Scholar]
  28. Riesco R, Trujillo ME. Update on the proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2024; 74:006300 [View Article] [PubMed]
     [Google Scholar]
  29. Sahoo AK, Vivek-Ananth RP, Chivukula N, Rajaram SV, Mohanraj K et al. T9GPred: a comprehensive computational tool for the prediction of type 9 secretion system, gliding motility, and the associated secreted proteins. ACS Omega 2023; 8:34091–34102 [View Article]
     [Google Scholar]
  30. Kharade SS, McBride MJ. Flavobacterium johnsoniae PorV is required for secretion of a subset of proteins targeted to the type IX secretion system. J Bacteriol 2015; 197:147–158 [View Article] [PubMed]
     [Google Scholar]
  31. Olsen AB, Spilsberg B, Nilsen HK, Lagesen K, Gulla S et al. Tenacibaculum piscium sp. nov., isolated from skin ulcers of sea-farmed fish, and description of Tenacibaculum finnmarkense sp. nov. with subdivision into genomovars finnmarkense and ulcerans. Int J Syst Evol Microbiol 2020; 70:6079–6090 [View Article]
     [Google Scholar]
  32. Piñeiro-Vidal M, Gijón D, Zarza C, Santos Y. Tenacibaculum dicentrarchi sp. nov., a marine bacterium of the family flavobacteriaceae isolated from European sea bass. Int J Syst Evol Microbiol 2012; 62:425–429 [View Article] [PubMed]
     [Google Scholar]
  33. Suzuki M, Nakagawa Y, Harayama S, Yamamoto S. Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 2001; 51:1639–1652 [View Article] [PubMed]
     [Google Scholar]
  34. Bernardet J-F, Nakagawa Y, Holmes B. Proposed minimal standards for describing new taxa of the family flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article] [PubMed]
     [Google Scholar]
  35. Avendaño-Herrera R, Irgang R, Núñez S, Romalde JL, Toranzo AE. Recommendation of an appropriate medium for in vitro drug susceptibility testing of the fish pathogen Tenacibaculum maritimum. Antimicrob Agents Chemother 2005; 49:82–87 [View Article] [PubMed]
     [Google Scholar]
  36. BLIGH EG, DYER WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37:911–917 [View Article] [PubMed]
     [Google Scholar]
  37. Tindall BJ, Sikorski J, Smibert RM, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology Washington, DC, USA: John Wiley & Sons, Ltd; 2007 pp 330–393 [View Article]
     [Google Scholar]
  38. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. In MIDI Inc vol 101 1990
     [Google Scholar]
  39. Vieira S, Huber KJ, Neumann-Schaal M, Geppert A, Luckner M et al. Usitatibacter rugosus gen. nov., sp. nov. and Usitatibacter palustris sp. nov., novel members of Usitatibacteraceae fam. nov. within the order nitrosomonadales isolated from soil. Int J Syst Evol Microbiol 2021; 71: [View Article]
     [Google Scholar]
  40. Lagadec E, Kahrs IEB, Frisch K, Duesund H, Nylund A et al. Improved growth media for isolation and identification of fish pathogenic Tenacibaculum spp. Microorganisms 2025; 13:1567 [View Article] [PubMed]
     [Google Scholar]
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Tenacibaculum salmonis sp. nov., isolated from Atlantic salmon (Salmo salar L.) fish farmed in Chile
Int J Syst Evol Microbiol 75, 006963 (2025); https://doi.org/10.1099/ijsem.0.006963
/content/journal/ijsem/10.1099/ijsem.0.006963
/content/journal/ijsem/10.1099/ijsem.0.006963
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