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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 11

sp. nov., a novel thermophilic member of the family with the ability of ferric iron reduction from deep-sea hydrothermal sulphide No Access

Abstract

A thermophilic, strictly anaerobic, Gram-stain-negative and ferric iron-reducing bacterium, designated as DY30410, was isolated from a hydrothermal sulphide sample collected from the Southwest Indian Ocean Ridge during the cruise DY30 of R/V . Cells of strain DY30410 were rod-shaped with rounded ends and motile with one or more laterally inserted flagellum (flagella). Strain DY30410 grew optimally at pH 6.5–7.0, 50 °C, with salinity (sea salts) of 35–45 g l. Ferric oxyhydroxide, ferric chloride, thiosulphate and elemental sulphur can be used as electron acceptors. Glucose and starch are used for growth. Elemental sulphur or yeast extract is not necessary. The principal fatty acids of strain DY30410 were iso-C, C, 3OH-iso-C and anteiso-C. No respiratory quinone was detected. Phylogenetically, strain DY30410 branched within the family with DY22619 being its closest phylogenetic relative (98.4% similarity), followed by the type species MV1087 (97.2%). Strain DY30410 has a draft genome size of 2.57 Mbp and has 2,852 predicted coding sequences. The genome DNA G+C content of strain DY30410 was 29.2 mol%. The DNA–DNA hybridization between strain DY30410 and its closest relatives, DY22619 or MV1087, was all 45.5%. The average nucleotide identity values between strain DY30410 and DY22619 and MV1087 were 91.9 and 91.3%, respectively. Phylogenomic analysis also showed that strain DY30410 branched within the family with DY22619 and MV1087 being its closest relatives. On the basis of phenotypic, phylogenetic and chemotaxonomic characteristics, we propose a representative of a new species in the genus , for which the name sp. nov. is proposed. The type strain is DY30410 (=KCTC 25912=MCCC 1A01531).

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Keyword(s): anaerobic , Caloranaerobacter longqiensis sp. nov. , Indian Ocean and iron reduction
Funding
This study was supported by the:
  • National Key Research and Development Program of China (Award 2021YFF0501304)
    • Principal Award Recipient: XiangZeng
  • Scientific Research Foundation of Third Institute of Oceanography, MNR, China (Award 2022009)
    • Principal Award Recipient: GuangyuLi
  • National Key Research and Development Program of China (Award 2022YFC2804100)
    • Principal Award Recipient: GuangyuLi
© 2025 The Authors
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2025-12-15

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Caloranaerobacter longqiensis sp. nov., a novel thermophilic member of the family Thermohalobacteraceae with the ability of ferric iron reduction from deep-sea hydrothermal sulphide
Int J Syst Evol Microbiol 75, 006962 (2025); https://doi.org/10.1099/ijsem.0.006962
/content/journal/ijsem/10.1099/ijsem.0.006962
/content/journal/ijsem/10.1099/ijsem.0.006962
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