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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 11

Description of two novel species of the genus : sp. nov. and sp. nov., isolated from soil of the Qinghai–Tibet Plateau No Access

Abstract

Four strains (LJ668/LJ756 and CY350/CY353) belonging to the genus were isolated from the Qinghai–Tibet Plateau soils. Cells were aerobic, Gram-stain-negative, rod-shaped, yellow-pigmented, non-motile and non-spore-forming. According to the 16S rRNA gene sequence alignment, the strain pair LJ668/LJ756 shared the highest similarities to T68 (98.09%) and SUR2 (98.09%), and the other one (CY350/CY353) to KCTC 12483 (97.98/98.13%). The phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that these four strains generated two separate clades in genus . The digital DNA–DNA hybridization and average nucleotide identity values of four novel isolates with other members of the genus were far below 70.0% and 95.0–96.0 % thresholds. The genomic DNA G+C content of LJ668 and CY350 is both 35.0 mol%. The major cellular fatty acids of the two pair strains are -C and summed feature 3 (C ω7c and/or C ω6c). MK-6 is the only respiratory quinone for strains LJ668 and CY350. The polar lipids of strain LJ668 are phosphatidylethanolamine (PE), L1–5 and AL1–5, and the strain CY350 is PE, L1–4, AL1–2, diphosphatidylglycerol, phosphoglycolipid and GL1–3. According to the results of genotypic, phenotypic and biochemical analyses, these four strains represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains are LJ668 (=CGMCC 1.17165=JCM 33877) and CY350 (=GDMCC 1.3790=KCTC 25690), respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Chryseobacterium , genomic analysis , phylogenetic analysis , Qinghai–Tibet Plateau and soil
Funding
This study was supported by the:
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principal Award Recipient: JianguoXu
© 2025 The Authors
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2025-11-05
2025-12-16

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Description of two novel species of the genus Chryseobacterium: Chryseobacterium chendengshani sp. nov. and Chryseobacterium wangxinyae sp. nov., isolated from soil of the Qinghai–Tibet Plateau
Int J Syst Evol Microbiol 75, 006941 (2025); https://doi.org/10.1099/ijsem.0.006941
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