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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 7

gen. nov., sp. nov., a thermo-halophilic bacterium isolated from a hot spring No Access

Abstract

A novel Gram-stain-negative, motile and thermo-halophilic rod-shaped bacterium SMD15-11 was isolated from Seokmodo hot spring in Incheon, Republic of Korea. The strain grew at concentrations of 0.5–7% (w/v) NaCl (optimum at 3%), at pH 5.0–9.0 (optimum at 7.0) and in a temperature range of 30–60 °C (optimum at 50 °C). Strain SMD15-11 shared the highest 16S rRNA gene sequence percentage with 96CJ10356 (92.5%). Both the phylogenetic and phylogenomic analyses showed that strain SMD15-11 clustered with members, demonstrating its close relatedness to the type species of the family. The genome-relatedness indices between strain SMD15-11 and other type species of the family were in the ranges of 67.2–69.1% for average nucleotide identity, 59.3–62.8% for average amino acid identity and 15.9–24.8% for digital DNA–DNA hybridization, which were significantly below the cut-off values for the genus delineation. The genome comprised 3,444,746 bp with a G+C content of 60.1 mol%. The polar lipids contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, four unidentified phospholipids, two unidentified aminophospholipids and two unidentified lipids. The predominant fatty acids are C , C, C and summed feature 3, comprising C C . The polyphasic taxonomic properties indicate that the strain represents a novel genus and species in the family for which the name gen. nov., sp. nov. is proposed. The type strain is SMD15-11 (=KCTC 8289=NBRC 116488).

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  • Accepted:
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Keyword(s): hot spring , halophile , polyphasic taxonomy , Thermohahella , Thermohahella caldifontis and thermophile
Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR202402105)
    • Principal Award Recipient: Sang-JaeLee
  • Ministry of Health and Welfare (Award RS-2023-KH141233)
    • Principal Award Recipient: Sang-JaeLee
  • Ministry of Oceans and Fisheries (Award KIMST-20210646)
    • Principal Award Recipient: Sang-JaeLee
  • Ministry of Trade, Industry and Energy (Award 20015807)
    • Principal Award Recipient: Sang-JaeLee
© 2025 The Authors
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2026-04-22

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Thermohahella caldifontis gen. nov., sp. nov., a thermo-halophilic bacterium isolated from a hot spring
Int J Syst Evol Microbiol 75, 006843 (2025); https://doi.org/10.1099/ijsem.0.006843
/content/journal/ijsem/10.1099/ijsem.0.006843
/content/journal/ijsem/10.1099/ijsem.0.006843
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