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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 7

sp. nov., a novel actinobacterium isolated from the frass of larvae No Access

Abstract

Four actinobacterial strains, designated NEAU-PBA1, NEAU-PB14, NEAU-PB65 and NEAU-PB77, were isolated from the frass of collected from Harbin, Heilongjiang Province, China. These strains were Gram-stain positive, aerobic and produced branched substrate mycelium and aerial hyphae, without producing diffusible pigments in the tested media. Upon maturity, spore chains formed on the aerial hyphae, while the mycelium fragmented with age. The genome sizes of these four strains ranged from 7.41 to 7.49 Mbp, with the genomic DNA G+C content varying from 71.8% to 72.9%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these four strains belonged to the genus and exhibited high sequence similarity with Mg02. The average nucleotide identity and digital DNA–DNA hybridization values between these four isolates exceeded the threshold for species delineation, while the values between the four isolates and their close relatives were below the threshold. The predominant menaquinones in strain NEAU-PBA1 are MK-10 (H, H, H) and MK-10, while the phospholipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, phosphatidylcholine, one phospholipid and one lipid. The major cellular fatty acids comprised -C, -C, -C and C 9. Based on polyphasic taxonomic analysis, strains NEAU-PBA1, NEAU-PB14, NEAU-PB65 and NEAU-PB77 were identified as a novel species within the genus , and the name sp. nov. was proposed. The type strain is NEAU-PBA1 (=MCCC 1K08877=JCM 37021).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Nocardiopsis , polyphasic taxonomy and Protaetia brevitarsis
Funding
This study was supported by the:
  • Research Team Project of Natural Science Foundation of Heilongjiang Province (Award TD2022C002)
    • Principle Award Recipient: YongqiangLiu
  • National Key Research and Development Program of China (Award 2022YFD1700205)
    • Principle Award Recipient: XiangjingWang
  • Key Programme (Award U22A20483)
    • Principle Award Recipient: XiangjingWang
  • Key Programme (Award 32030090)
    • Principle Award Recipient: WenshengXiang
© 2025 The Authors
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Nocardiopsis protaetiae sp. nov., a novel actinobacterium isolated from the frass of Protaetia brevitarsis larvae
Int J Syst Evol Microbiol 75, 006832 (2025); https://doi.org/10.1099/ijsem.0.006832
/content/journal/ijsem/10.1099/ijsem.0.006832
/content/journal/ijsem/10.1099/ijsem.0.006832
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