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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 6

sp. nov. and sp. nov., two -cryptoxanthin-producing bacteria isolated from intestinal contents of No Access

Abstract

Four Gram-stain-positive, facultative anaerobic, yellow-pigmented and short rod-shaped strains, designated zg-Y625, zg-Y843, zg-Y1090 and zg-Y1211, were isolated from the intestinal contents of in Qinghai Province, PR China. Strains zg-Y625 and zg-Y1090 showed the highest 16S rRNA gene sequence similarities of 99.7% and 99.9% to DSM 112390, respectively, followed by 99.2% and 99.5% to JCM 14341 and 99.1% and 99.0% to LMG 32277. Phylogenetic analyses based on 16S rRNA gene sequences and phylogenomic analyses using whole-genome sequences revealed that these four strains belong to the genus , forming two separate clades distinct from all other known species. The genome sizes of strains zg-Y625 and zg-Y1090 were 3.26 and 3.07 Mb, respectively, with DNA G+C contents of 70.5 and 70.9 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between each of the novel strains and the available members of the genus were all below the species thresholds. Both type strains contained diphosphatidylglycerol and phosphatidylglycerol as predominant polar lipids with one unidentified glycolipid for zg-Y625 and two unidentified glycolipids for zg-Y1090. The predominant respiratory quinone in zg-Y625 was MK-13, whilst in zg-Y1090, both MK-11 and MK-13 were identified as the major quinones. The major fatty acids (>10%) in strains zg-Y625 and zg-Y843 were anteiso-C, anteiso-C and iso-C, whereas for zg-Y1090 and zg-Y1211, the predominant fatty acids were anteiso-C and anteiso-C. Based on phenotypic, phylogenetic, genomic and chemotaxonomic data, two novel species in the genus are proposed, namely, sp. nov. (zg-Y625=GDMCC 1.3931=JCM 36203) and sp. nov. (zg-Y1090=GDMCC 1.3930=JCM 36205).

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  • Accepted:
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Keyword(s): Marmota himalayana , Microbacterium , novel species and phylogenetic analysis
Funding
This study was supported by the:
  • the Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: XuJianguo
© 2025 The Authors
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2025-06-20
2025-07-10
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Microbacterium jiangjiandongii sp. nov. and Microbacterium wangruii sp. nov., two β-cryptoxanthin-producing bacteria isolated from intestinal contents of Marmota himalayana
Int J Syst Evol Microbiol 75, 006821 (2025); https://doi.org/10.1099/ijsem.0.006821
/content/journal/ijsem/10.1099/ijsem.0.006821
/content/journal/ijsem/10.1099/ijsem.0.006821
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