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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 6

sp. nov., a novel methylotrophic methanol-utilizing bacterium isolated from the paddy soil No Access

Abstract

Four pink-pigmented bacteria, designated as strains sgz302003, sgz302004, sgz302005 and sgz302006, were isolated from a paddy soil enrichment. The cells were aerobic, Gram-stain-negative, motile and short rods. The ranges and optima of the temperature, NaCl concentration and pH for growth were 25–37 °C (optimum, 30 °C), 0–2% (w/v) (optimum, 0–0.5%) and pH 5.0–10.0 (optimum, pH 6.5), respectively. Major fatty acids were summed feature 8 (C 7 and/or C 6). Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains were affiliated with the genus and were most closely related to AR24 (99.2% sequence similarity), ORS 206 (97.7%) and PR1016A (97.4%). The methanol dehydrogenase gene was absent, but was present. Phylogenomic tree supported that these four strains formed a distinct clade within . Q-10 was detected as the only respiratory quinone in the studied strains. The genomic DNA G+C content was 70.7–70.8 %. The orthologous average nucleotide identity and digital DNA–DNA hybridization values between the four strains and AR24 were 91.8–91.9% and 53.0–53.3%, respectively, while those with ORS 206 were 86.3–86.5% and 25.5%. Phylogenomic, physiological and chemotaxonomic analyses indicate that the four strains represent a novel species within the genus , for which the name sp. nov. is proposed, with strain sgz302003 (= MCCC 1K09384=KCTC 8693) as the type strain.

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Keyword(s): Methylobacterium oryzisoli sp. nov. , methylotrophs , paddy soil and polyphasic taxonomy
Funding
This study was supported by the:
  • Science and technology special project of Fujian Province (Award 2023L3022)
    • Principle Award Recipient: NotApplicable
  • Special project for outstanding scientific and technological innovation talents of Fujian Academy of Agricultural Sciences (Award YCZX202407)
    • Principle Award Recipient: Guo-HongLiu
  • National Nature Science Foundation of China (Award 42477274)
    • Principle Award Recipient: Guo-HongLiu
© 2025 The Authors
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2025-06-06
2025-06-24
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Methylobacterium oryzisoli sp. nov., a novel methylotrophic methanol-utilizing bacterium isolated from the paddy soil
Int J Syst Evol Microbiol 75, 006805 (2025); https://doi.org/10.1099/ijsem.0.006805
/content/journal/ijsem/10.1099/ijsem.0.006805
/content/journal/ijsem/10.1099/ijsem.0.006805
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