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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 5

Description of gen. nov., sp. nov., a novel actinobacterium isolated from riverside soil No Access

Abstract

A Gram-positive, oval-shaped and non-motile strain, designated MMS21-DH1MA12, was isolated from riverside soil and subjected to polyphasic taxonomic characterization. Strain MMS21-DH1MA12 grew optimally in tryptic soy agar, forming yellow-coloured, convex and smooth colonies. The optimal growth conditions of the strain were 30 °C, pH 7 and absence of NaCl. Strain MMS21-DH1MA12 shared the highest 16S rRNA gene sequence similarity with NS18 (97.3%), and then with 6408 J-67 (97.1%) and CGMCC 1.11101 (96.9%), all belonging to the family . Strain MMS21-DH1MA12 had a genome size of 2.4 Mbp and G+C content of 62.6%. The digital DNA–DNA hybridization (dDDH), orthologous average nucleotide identity (orthoANI) and average amino acid identity (AAI) values between strain MMS21-DH1MA12 and related type strains of the genera within the family were no higher than 22.2%, 71.54% and 60.19%, respectively. The dDDH, orthoANI and AAI values of MMS21-DH1MA12 with related taxa were no higher than 22.2%, 71.54% and 61.96%, thus showing a distant relationship of the strain with other members of . It was also notable that the species sharing the highest similarities were different for all three indices. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The main cellular fatty acids were -C, -C and -C. The predominant menaquinones were MK-10 and MK-11. The combination of physiological and biochemical properties, as well as chemotaxonomic features, clearly separated strain MMS21-DH1MA12 from related taxa of . It is, thus, evident that MMS21-DH1MA12 represents a novel species in a novel genus, for which the name gen. nov., sp. nov. is proposed (type strain=MMS21-DH1MA12 = KCTC 49751 = LMG 32525=JCM 37009).

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  • Accepted:
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Keyword(s): average amino acid identity (AAI) , Humidisolicoccus flavus , Microbacteriaceae and riverside soil
Funding
This study was supported by the:
  • National Institute of Biological Resources
    • Principal Award Recipient: SeungBum Kim
© 2025 The Authors
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2025-05-21
2026-02-07

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Description of Humidisolicoccus flavus gen. nov., sp. nov., a novel actinobacterium isolated from riverside soil
Int J Syst Evol Microbiol 75, 006786 (2025); https://doi.org/10.1099/ijsem.0.006786
/content/journal/ijsem/10.1099/ijsem.0.006786
/content/journal/ijsem/10.1099/ijsem.0.006786
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