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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 5

sp. nov., isolated from rocky soil of Sichang Island No Access

Abstract

A novel actinobacterium, designated strain SC052, was isolated from rocky soil collected from Sichang Island, Chonburi Province, Thailand. Morphological and chemotaxonomic analyses indicated that strain SC052 belongs to the genus . The strain exhibited extensively branched substrate mycelia and produced short chains of four to six spores on the aerial mycelia. The cell wall contained -diaminopimelic acid, while ribose, arabinose, glucose and galactose were detected in the whole-cell hydrolysate. The identified polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, one unidentified glycolipid, two unidentified lipids and three unidentified phospholipids. The menaquinone was MK-8 (H4-cycl). The major cellular fatty acids included C, C, C, 10-methyl C tuberculostearic acid, C  9c, summed feature 3 and summed feature 6. Mycolic acids were also detected. A search of the 16S rRNA gene sequence revealed that strain SC052 exhibited the highest similarity (99.7%) to NBRC 100135. Phylogenetic analysis based on the gene and phylogenomic tree construction further supported that NBRC 100135 is the closest phylogenetic neighbour of strain SC052. Comparative genomic analyses between strain SC052 and NBRC 100135 revealed digital DNA–DNA hybridization values of 47.9% and average nucleotide identity values of 91.5% (ANIb) and 92.8% (ANIm), all of which fall below the threshold used to delineate strains of the same species. Based on phenotypic and genomic analyses, strain SC052 is proposed as a novel species within the genus , for which the name sp. nov. is proposed (=TBRC 14541, =NBRC 115195).

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Keyword(s): actinomycetes , island , Nocardia , novel species , polyphasic taxonomy and rocky soil
Funding
This study was supported by the:
  • Thailand Science Research and Innovation Fund Chulalongkorn University (Award HEA_FF_68_163_3300_010)
    • Principal Award Recipient: WongsakornPhongsopitanun
© 2025 The Authors
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2026-03-13

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Nocardia sichangensis sp. nov., isolated from rocky soil of Sichang Island
Int J Syst Evol Microbiol 75, 006784 (2025); https://doi.org/10.1099/ijsem.0.006784
/content/journal/ijsem/10.1099/ijsem.0.006784
/content/journal/ijsem/10.1099/ijsem.0.006784
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