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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 6

sp. nov. and sp. nov., isolated from marine coastal sediment No Access

Abstract

Two novel Gram-stain-negative, rod-shaped, facultatively anaerobic and non-motile bacterial strains, designated as S1116L3 and S4756, were isolated from the marine sediment of Jingzi Port, Weihai, China. Strain S1116L3 grew at temperatures 15–37 ℃ within pH 5.5–9.0 and in the presence of 1.0%–10.0% (w/v) NaCl. Optimal growth required 3.0% (w/v) NaCl at 30 ℃ and pH 7.0. Strain S4756 grew at 15–37 ℃, pH 6.0–9.0 and in the presence of 1.0%–10.0% (w/v) NaCl. Optimal growth appeared with 2.0% (w/v) NaCl at 30 ℃ and pH 7.0. Strains S1116L3 and S4756 had a major fatty acid of C 7. The predominant respiratory quinone of the two strains was Q10. The major polar lipids detected in the two strains were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The phylogenetic analysis based on 16S rRNA gene sequences placed the strains S1116L3 and S4756 into the genus . The comparison of 16S rRNA gene sequence similarities revealed that strains S1116L3 and S4756 had the closest relative with KCTC 32014 (98.4% and 97.9%, respectively). These values were below the species delineation threshold of 98.6%. The average nucleotide identity (ANI) values based on the genome similarity between strain S1116L3 and species of the genus ranged from 74.6% to 82.8%, while the ANI values ranged from 74.3% to 82.5% for strain S4756, which is significantly lower than the species delineation threshold of 95%. The DNA G+C content of both strains is 63.0%. Based upon the results presented in this study, strains S1116L3 and S4756 represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed with the type strains S1116L3 (=KCTC 8178=MCCC 1H01426=SDUM 031094) and S4756 (=KCTC 8179=MCCC 1H01425=SDUM 031096), respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , marine environment , polyphasic taxonomy and Roseovarius
Funding
This study was supported by the:
  • Natural Science Foundation of Shandong Province (Award ZR2023QD187)
    • Principle Award Recipient: JingZhang
  • China Postdoctoral Science Foundation (Award 2023M732055)
    • Principle Award Recipient: ZhenxingXu
  • the Science and Technology Fundamental Resources Investigation Program (Award 2022FY101100)
    • Principle Award Recipient: Zong-JunDu
© 2025 The Authors
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2025-06-03
2025-06-13
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Roseovarius roseus sp. nov. and Roseovarius maritimus sp. nov., isolated from marine coastal sediment
Int J Syst Evol Microbiol 75, 006782 (2025); https://doi.org/10.1099/ijsem.0.006782
/content/journal/ijsem/10.1099/ijsem.0.006782
/content/journal/ijsem/10.1099/ijsem.0.006782
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