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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 5

sp. nov., a new antibiotic-producing actinomycete isolated from marine sediment No Access

Abstract

An actinomycete strain that produces a new antibiotic haneummycin, designated KM77-8, was isolated from marine sediment collected from Tokyo Bay near Haneda Airport, Japan. This strain was phylogenetically related to NBRC 12807, with the highest similarity (99.65%) based on 16S rRNA gene sequences. In contrast, genome-based comparisons between strain KM77-8 and JCM 4403 (=NBRC 12807) revealed that average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values were 87.29% and 33.00 %, respectively. Moreover, genome-based comparisons between strain KM77-8 and other known species, including close relatives (more than 98.7% similarities of 16S rRNA gene sequences), showed that their ANI and dDDH values were <90% and <70%, respectively. The DNA G+C content of strain KM77-8 was 71.34%. The whole-cell hydrolysates of strain KM77-8 contained -diaminopimelic acid, galactose, glucose, mannose and ribose. The major menaquinones (MKs) of this strain were MK-9(H) and MK-9(H), and the major fatty acids (>10%) were anteiso-C, iso-C, C, iso-C and summed feature 3 (C cC c). Several phenotypic differences were observed between strain KM77-8 and NBRC 12807. Furthermore, supernatant and methanol extract of not NBRC 12807 but strain KM77-8 displayed antibacterial activity against and contained haneummycin. On the basis of the polyphasic data, we propose strain KM77-8 as a novel species of the genus , named sp. nov. The type strain is KM77-8 (=NBRC 115995=DSM 115857).

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Keyword(s): Actinomycetes , antibiotic , marine sediment and Streptomyces
© 2025 The Authors
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2025-05-12
2025-06-22
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Streptomyces haneummycinicus sp. nov., a new antibiotic-producing actinomycete isolated from marine sediment
Int J Syst Evol Microbiol 75, 006777 (2025); https://doi.org/10.1099/ijsem.0.006777
/content/journal/ijsem/10.1099/ijsem.0.006777
/content/journal/ijsem/10.1099/ijsem.0.006777
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