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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 5

Identification of sp. nov. isolated from hospital air No Access

Abstract

The genus comprises Gram-negative bacteria widely distributed in the environment and can cause human infections. However, the genomic characteristics and pathogenicity of remain poorly studied. Two new Gram-negative, mesothermic, aerobic and coccoid strains (SXEYE001 and SXEYE002) were isolated from hospital air. Colonies grown on R2A plates at 28 °C for 3 days were round with neat, raised edges and a pale pink colour. Sequencing of the 16S rRNA gene revealed that the two strains are 98.4% similar to ‘’ BUT 13. The 16S rRNA gene sequence was observed to be 97.4% similar to N75. Genome-based phylogeny demonstrated that CPCC 101081 and ‘’ BUT-13species are close relatives to strains SXEYE001 and SXEYE002. The average nucleotide identity between the strains and species of the genus was <95.0%. The G+C contents of strains SXEYE001 and SXEYE002 were calculated to be 67.8mol% and 67.9mol%, respectively. The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, with coenzyme Q-10 serving as the primary respiratory quinone. Based on comprehensive genotypic, phenotypic, phylogenetic and phylogenomic analyses, we concluded that strains SXEYE001 and SXEYE002 were indicative of a novel species within the genus , for which the name sp. nov. is proposed, with SXEYE001 (=GDMCC 1.3626=JCM 35902) as the type strain.

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Keyword(s): hospital air , Roseomonas xinghualingensis and systematics
Funding
This study was supported by the:
  • Shanxi Provincial Eye Hospital Innovation Fund Project (Award Q202203)
    • Principal Award Recipient: ZhimingKang
  • Shanxi Collaborative Innovation Center of High Value-added Utilization of Coal-related Wastes (Award B202201)
    • Principal Award Recipient: KuiDong
  • Natural Science Research Project of Shanxi Provincial Department of Science and Technology (Award 20210302123343)
    • Principal Award Recipient: KuiDong
© 2025 The Authors
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2025-05-08
2026-02-16

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Identification of Roseomonas xinghualingensis sp. nov. isolated from hospital air
Int J Syst Evol Microbiol 75, 006775 (2025); https://doi.org/10.1099/ijsem.0.006775
/content/journal/ijsem/10.1099/ijsem.0.006775
/content/journal/ijsem/10.1099/ijsem.0.006775
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