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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 5

sp. nov. and subsp. subsp. nov. No Access

Abstract

In September 2018, Hurricane Florence resulted in major flooding in North Carolina, USA. Efforts to isolate and other spp. from Hurricane Florence floodwaters repeatedly yielded non-haemolytic -like isolates that could not be readily assigned to known taxa. Whole-genome sequence analyses against the 28 currently known species confirmed that the isolates constitute two new taxa within the genus . Taxon I, with one isolate, showed the highest similarity to with an average nucleotide identity of 85.3±4.4% and an DNA–DNA hybridization (DDH) of 32.4% (range: 30–35%), differing from the latter by its ability to reduce nitrate, ferment -ribose and sucrose, and by its inability to produce catalase or ferment -trehalose and -lactose. Taxon II, represented by 11 isolates, showed the highest similarity to with an average nucleotide identity of 92.64±3.8% and an DDH of 49.9% (range: 47.3–52.5%), differing from the latter by its ability to ferment -arabinose and its inability to ferment -rhamnose, -galactose, -lactose and -melibiose. The names sp. nov. and subsp. subsp. nov. are proposed for taxon I and II, respectively, with type strains CLIP 2022/01175 (F6L-1A=CIP 112444 = DSM 117029) and CLIP 2022/01000 (F66L-1A=CIP 112443 = DSM 117030), respectively. Both taxa lack known pathogenic islands, suggesting a lack of pathogenicity for humans.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): average nucleotide identity (ANI) , Firmicutes , in silico DNA–DNA hybridization (isDDH) , Listeria , new taxa and whole-genome sequencing
Funding
This study was supported by the:
  • Department of Food, Nutrition and Bioprocessing Sciences, North Carolina State UniversityMetro North Hospital and Health Service
    • Principle Award Recipient: NotApplicable
  • College of Agriculture and Life Sciences, North Carolina State University
    • Principle Award Recipient: NotApplicable
  • National Institute of Food and Agriculture (Award 2018-07464)
    • Principle Award Recipient: NotApplicable
  • International Life Sciences Institute (ILSI) North America Food Microbiology Committee
    • Principle Award Recipient: NotApplicable
  • Santé Publique France
    • Principle Award Recipient: NotApplicable
  • Inserm
    • Principle Award Recipient: NotApplicable
  • Institut Pasteur
    • Principle Award Recipient: NotApplicable
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2025-06-22
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Listeria tempestatis sp. nov. and Listeria rocourtiae subsp. hofi subsp. nov.
Int J Syst Evol Microbiol 75, 006774 (2025); https://doi.org/10.1099/ijsem.0.006774
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/content/journal/ijsem/10.1099/ijsem.0.006774
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