Skip to content
1887

Abstract

A novel basidiomycete yeast, designated as NYNU 2211380, was isolated from a leaf of collected in the Baotianman Nature Reserve, Henan Province, central China. Phylogenetic analyses of the D1/D2 domain of the LSU rRNA gene and the internal transcribed spacer (ITS) region revealed its close relationship to , differing by 3.4% mismatches (14 substitutions and 6 gaps) in the D1/D2 domain and 12.4% mismatches (35 substitutions and 37 gaps) in the ITS region. The novel strain also exhibits distinct physiological traits, including an inability to assimilate maltose and cellobiose and the ability to utilize -xylose, -ribose, glycerol, ribitol, -mannitol, -lactate and -gluconate. Based on phylogenetic and phenotypic data, the strain represents a new species in the genus , for which the name sp. nov. (Holotype: CICC 33585; MycoBank: MB 857392) is proposed.

Funding
This study was supported by the:
  • State Key Laboratory of Motor Vehicle Biofuel Technology, Henan Tianguan Enterprise Group Co., Ltd., China (Award 2018001)
    • Principal Award Recipient: Rui-XiuWang
  • National Natural Science Foundation of China (Award 31570021)
    • Principal Award Recipient: Feng-LiHui
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.006763
2025-04-22
2026-02-07

Metrics

Loading full text...

Full text loading...

References

  1. Li A-H, Yuan F-X, Groenewald M, Bensch K, Yurkov AM et al. Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: proposal of two new orders, three new families, eight new genera and one hundred and seven new species. Stud Mycol 2020; 96:17–140 [View Article] [PubMed]
    [Google Scholar]
  2. Wang Q-M, Yurkov AM, Göker M, Lumbsch HT, Leavitt SD et al. Phylogenetic classification of yeasts and related taxa within pucciniomycotina. Stud Mycol 2015; 81:149–189 [View Article] [PubMed]
    [Google Scholar]
  3. Bai FY, Takashima M, Hamamoto M, Nakase T. Sporobolomyces yunnanensis sp. nov., a Q-10(H2)-containing yeast species with a close phylogenetic relationship to Erythrobasidium hasegawianum. Int J Syst Evol Microbiol 2001; 51:231–235 [View Article] [PubMed]
    [Google Scholar]
  4. Zang W, Liu CY, Xie GF, Shen C, Liu XZ et al. Resources and species diversity of the phyllosphere yeasts from the yandang mountains in zhejiang province. Acta Ecologica Sinica 2018; 38:3920–3930 [View Article]
    [Google Scholar]
  5. Srisuk N, Nutaratat P, Surussawadee J, Limtong S. Yeast communities in sugarcane phylloplane. Microbiology 2019; 88:353–369 [View Article]
    [Google Scholar]
  6. Into P, Pontes A, Sampaio JP, Limtong S. Yeast diversity associated with the phylloplane of corn plants cultivated in Thailand. Microorganisms 2020; 8:80 [View Article] [PubMed]
    [Google Scholar]
  7. Hu WT, Chu SB, Li Y, Hui FL. Hyphopichia xiaguanensis f.a., sp. nov., an ascomycetous yeast species isolated from plant leaves. Int J Syst Evol Microbiol 2022; 72:005398 [View Article] [PubMed]
    [Google Scholar]
  8. Lu YF, Chai CY, Hui FL. Two new phyllospheric species of Colacogloea (colacogloeaceae, pucciniomycotina) identified in China. MycoKeys 2024; 101:81–94 [View Article] [PubMed]
    [Google Scholar]
  9. Qiao YZ, Liu S, Niu QH, Hui FL. Three new Dioszegia species (bulleribasidiaceae, tremellales) discovered in the phylloplane in China. MycoKeys 2024; 101:313–328 [View Article] [PubMed]
    [Google Scholar]
  10. Guo QC, Liu S, Qiao YZ, Hui FL. Three new species of Teunia (cryptococcaceae, tremellales) identified through phenotypic and phylogenetic analyses. MycoKeys 2024; 105:139–153 [View Article] [PubMed]
    [Google Scholar]
  11. Nakase T, Takashima MA. Simple procedure for the high frequency isolation of new taxa of ballistosporous yeasts living on the surfaces of plants. RIKEN Review 1993; 3:33–34
    [Google Scholar]
  12. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. eds The Yeasts, a Taxonomic Study, 5th ed. vol 1 Amsterdam: Elsevier; 2011 pp 87–110 [View Article]
    [Google Scholar]
  13. do Carmo-Sousa L, Phaff HJ. An improved method for the detection of spore discharge in the sporobolomycetaceae. J Bacteriol 1962; 83:434–435 [View Article] [PubMed]
    [Google Scholar]
  14. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 1998; 73:331–371 [View Article] [PubMed]
    [Google Scholar]
  15. White TJ, Bruns TD, Lee SJWT, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Innis MA, Gelfand DH, Sninsky JJ, White TJ. PCR Protocols, a Guide to Methods and Applications New York: Academic Press; 1990315–322
    [Google Scholar]
  16. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [View Article] [PubMed]
    [Google Scholar]
  17. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DJ. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article] [PubMed]
    [Google Scholar]
  18. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
    [Google Scholar]
  19. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
    [Google Scholar]
  20. Nei M, Kumar S. Molecular Evolution and Phylogenetics New York: Oxford University Press; 2000
    [Google Scholar]
  21. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article] [PubMed]
    [Google Scholar]
  22. Schoutteten N, Yurkov A, Leroux O, Haelewaters D, Van Der Straeten D et al. Diversity of colacosome-interacting mycoparasites expands the understanding of the evolution and ecology of Microbotryomycetes. Stud Mycol 2023; 106:41–94 [View Article] [PubMed]
    [Google Scholar]
  23. Schoutteten N, Yurkov A, Spirin V, Savchenko A, Aime MC et al. Examination of mycoparasites reveals a new type of host-parasite interface and rearranges the taxonomy of occultifur and microsporomyces (cystobasidiomycetes, basidiomycota). Stud Mycol 2024; 109:451–486 [View Article]
    [Google Scholar]
  24. Timling I, Walker DA, Nusbaum C, Lennon NJ, Taylor DL. Rich and cold: diversity, distribution and drivers of fungal communities in patterned-ground ecosystems of the North American Arctic. Mol Ecol 2014; 23:3258–3272 [View Article] [PubMed]
    [Google Scholar]
  25. Ort BS, Bantay RM, O’Grady PM. Fungal diversity associated with Hawaiian Drosophila host plants. PLoS One 2012; 7:e40550 [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.006763
Loading
/content/journal/ijsem/10.1099/ijsem.0.006763
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error