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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 4

gen. nov., sp. nov., a novel moderately halophilic, thermotolerant, bacterium isolated from a brine lake Open Access

Abstract

A novel, strictly anaerobic, slightly alkaliphilic, halotolerant, peptide- and amino acid-utilizing bacterial strain, SD1, was isolated from a hypersaline lake in Western Australia. The strain stained Gram-negative and was a motile, spore-forming rod. The strain grew between 15 and 50 °C (optimum 40 °C), 1–15% w/v sodium chloride (optimum 5%) and pH 6.0–10.0 (optimum 9.0). Major fatty acids included anteiso-C15 : 0 (24.9%), C14 : 0 dimethyl acetyl (13.2%), anteiso-C15 : 0 dimethyl acetyl (11.5%) and iso-C15 : 0 (10.4%). The DNA G+C content was 30.3 mol%. The isolate did not grow using any tested sugars but grew well on arginine and glycine. It is capable of using elemental sulfur and thiosulfate as alternate electron acceptors, but not sulfide, sulfate, nitrate or nitrite. 16S rRNA gene similarity indicates that the isolate is related to MRo-4 (94.33% identity). SD1 showed 76.18%–76.31% average nucleotide identity with other strains within the family . Phylogenetics, based on the 16S rRNA gene and whole-genome sequence, as well as phenotypic analysis, differentiates the isolate from close neighbors. We propose that SD1 represents a novel species in a new genus, which we have named gen. nov., sp. nov., type strain SD1 (DSM 117405 = TSD-443). From this work, we also propose repositioning of the genus to the family .

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  • Accepted:
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Keyword(s): anaerobes , Brine lakes , Dethiothermospora halolimnae , Thermohalobacteraceae and Western Australia transient lakes
Funding
This study was supported by the:
  • NASA Astrobiology Institute (Award 80NSSC18K1301)
    • Principal Award Recipient: E. SchmidtBritney
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2025-04-30
2026-03-06

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Dethiothermospora halolimnae gen. nov., sp. nov., a novel moderately halophilic, thermotolerant, bacterium isolated from a brine lake
Int J Syst Evol Microbiol 75, 006760 (2025); https://doi.org/10.1099/ijsem.0.006760
/content/journal/ijsem/10.1099/ijsem.0.006760
/content/journal/ijsem/10.1099/ijsem.0.006760
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