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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 75, Issue 4

gen. nov., sp. nov., a novel member of the family , isolated from pig manure No Access

Abstract

A Gram-positive, aerobic, non-motile coccoid bacterium, designated strain ZF39, was isolated from a farm in Quandian Village, Zhengzhou City, Henan Province, China, and subjected to taxonomic characterization. Optimal growth occurred at 32 ℃, pH 7.0 and 0–1% NaCl (w/v). Mannose and glucose were identified as the sugar components in the whole-cell hydrolysates. The strain’s peptidoglycan type was classified as A3 (-Dpm-Gly), and MK-9(H) was identified as the predominant respiratory quinone. Aminoglycolipid, aminophospholipid, glycolipids, phosphatidylglycerol and phospholipid were detected as the major polar lipids. The predominant cellular fatty acids were anteiso-C (48.7%) and iso-C (20.2%). Phylogenetic analysis based on 16S rRNA gene sequences placed strain ZF39 within the family . The strain formed a distinct genetic lineage adjacent to the genus , with 16S rRNA gene sequence similarities of 94.70% to CPCC 204711 and 94.56% to KCTC 49116. The genomic G+C content was 67.7 mol%. Genome comparisons revealed that the average nucleotide identity values between strain ZF39 and CPCC 204711, KCTC 49116, DSM 17626 were 72.50%, 71.98% and 72.36%, respectively. Moreover, phylogenetic analysis and phenotypic characterization supported the classification of strain ZF39 as a novel species of a new genus within the family . The name gen. nov., sp. nov. is proposed, with ZF39 (=CGMCC 1.60047=KCTC 59250=MCCC 1K08851) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ammonia-resistant strain , Ammonicoccus fulvus , pig manure , polyphasic identification , Propionibacteriaceae and taxonomy
Funding
This study was supported by the:
  • the Fundamental Research Fund of Henan Academy of Sciences (Award 241005023, 2406005013, 242205029, 230505002，220905012)
    • Principle Award Recipient: TaoGong
© 2025 The Authors
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2025-04-22
2025-05-22
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Ammonicoccus fulvus gen. nov., sp. nov., a novel member of the family Propionibacteriaceae, isolated from pig manure
Int J Syst Evol Microbiol 75, 006756 (2025); https://doi.org/10.1099/ijsem.0.006756
/content/journal/ijsem/10.1099/ijsem.0.006756
/content/journal/ijsem/10.1099/ijsem.0.006756
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