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Abstract

In this study, we re-evaluated the taxonomic relationship of and by using whole-genome comparative analysis. The 16S rRNA gene sequence analysis indicated that IFO 12511 showed high 16S rRNA gene sequence similarities to K95-5561 (99.86%), IFO 12513 (99.30%), IFO 12245 (99.14%), 03-723 (98.73%), A-T 5400 (98.67%) and ˂98.60% to others. The phylogenetic tree based on 16S rRNA gene sequences revealed that IFO 12511 clustered with K95-5561. The phylogenetic analysis based on whole-genome sequences further confirmed this result and indicated that IFO 12511 was closely related to K95-5561. However, the average nucleotide identity and digital DNA–DNA hybridization values between them were 96.14%/96.75% and 70.90%, respectively. These values are higher than 95–96% and 70% for the delineation of prokaryotic genomic species, confirming that and should belong to the same species. In addition, phenotypic and genome properties between NBRC 12511 and NBRC 16408 were very similar. Therefore, based on phenotypic, chemotaxonomic and molecular data, we propose [ 1 ] as a later heterotypic synonym of [ 2, 3 ].

Funding
This study was supported by the:
  • Changde Key Innovation Team for wetland biology and Environmental Ecology
    • Principal Award Recipient: WanshengZou
  • Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province
    • Principal Award Recipient: YunWang
  • the Science and Technology Innovation Program of Hunan Province (Award 2022RC1169)
    • Principal Award Recipient: BaiyuanLi
  • the project of Hunan Provincial Education Department (Award 24A0492)
    • Principal Award Recipient: WanshengZou
  • the project of Hunan Provincial Education Department (Award 23B0659)
    • Principal Award Recipient: PingMo
  • the Hunan Natural Science Foundation (Award 2024JJ7300)
    • Principal Award Recipient: PingMo
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2025-04-07
2025-12-10

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